Cytotoxic activity evaluation of some medicinal plants, selected from Iranian traditional medicine Pharmacopoeia to treat cancer and related disorders

Naghibi, Farzaneh; Khalaj, Amir; Mosaddegh, Mahmoud; Malekmohamadi, Maryam; Hamzeloo-Moghadam, Maryam

doi:10.1016/j.jep.2014.05.025

Cited by 31 publications

(33 citation statements)

References 47 publications

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“…The methanol extract of Hypericum scabrum has demonstrated cytotoxic activity in our previous studies ( 31 ). In the present study, cytotoxic activity of 3 different fractions of Hypericum scabrum L. has been investigated by MTT assay against some tumor cell lines (MCF-7, A-549, HT-29, and HepG-2).…”

Section: Objectivesmentioning

confidence: 82%

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Cytotoxic Activity and Apoptosis Induction of Hypericum scabrum L.

Hamzeloo-Moghadam¹,

Khalaj²,

Malekmohammadi³

2015

Iran Red Crescent Med J

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Background:One of the acquired biological hallmarks of tumor multistep development is the resistance of cancer cells to apoptosis; therefore, induction of apoptosis is an important therapeutic approach. Hypericum species are spread throughout the world and have been investigated for their biological properties.Objectives:Our previous studies had demonstrated cytotoxicity of Hypericum scabrum L. methanol extract against some tumor cell lines, suggesting the species for further studies. The objectives of the present study were to determine the most cytotoxic fraction of Hypericum scabrum L. and to assess the apoptosis induction ability of the most effective fraction as well as its methanol extract. The laboratory evidence has been presented to support the potency of Iranian Traditional Medicine (ITM) medicinal plants as a source of different biological activity surveys and drug discoveries.Materials and Methods:The present research is a descriptive study. The sampling strategy was based on ITM data of cancer phytotherapy. Hypericum scabrum was collected from Alborz province, Iran (2012) and the herbarium specimen was taxonomically identified. The petroleum ether, dichloromethane, and methanol fractions have been evaluated for cytotoxicity against M-CF7, A-549, HT-29, and HepG-2 cell lines through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT assay. The apoptosis induction ability has been assessed by activated caspase-3 inspection and Annexin V FITC/PI (propidium iodide) assays.Results:Di-chloromethane fraction demonstrated IC50 values of 25.72 μg/mL and 24.73 μg/mL against HT-29 and HepG-2 cell lines, respectively and IC50 values of petroleum ether fraction were 22.6 μg/mL and 18.31 μg/mL against HT-29 and HepG-2, respectively. The methanol fraction did not show cytotoxic activity. Both the methanol extract and the petroleum ether fraction of Hypericum scabrum L. revealed apoptosis induction ability.Conclusions:Considering the strong historical background about the therapeutic potential of the genus Hypericum and the considerable cytotoxic and apoptosis induction ability of Hypericum scabrum, this species is suggested for future biological studies.

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Section: Objectivesmentioning

confidence: 82%

“… a IC 50 values were reported from our former study ( 31 ). b IC 50 values greater than 100 μg/mL were considered as inactive.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic Activity and Apoptosis Induction of Hypericum scabrum L.

Hamzeloo-Moghadam¹,

Khalaj²,

Malekmohammadi³

2015

Iran Red Crescent Med J

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“…The MTT assay was used to evaluate the cytotoxicity of the extract. In MTT assay, the ability of the live cells is attributed to the conversion of the yellow MTT to purple formazan salt (Hamzeloo-Moghadam et al, 2013;Naghibi et al, 2014). The cells were exposed to the methanol extract (9,000 and 8,000 cells/well in 96-well plates for MCF-7 and MDA-MB-468 cells, respectively).…”

Section: Cell Culture and Cytotoxicity Assaymentioning

confidence: 99%

“…At the end of the experiment, DMSO was used to dissolve the formed formazan crystals and the absorbance was recorded at 570 nm. The IC50 was calculated afterwards (Hamzeloo-Moghadam et al, 2014).…”

Section: Cell Culture and Cytotoxicity Assaymentioning

confidence: 99%

Acroptilon repens induces apoptosis in human breast adenocarcinoma

Hamzeloo-Moghadam

Mosaddegh

Irani

2018

Bangladesh J Pharmacol

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Evaluating the effect of herbal extracts has been always interesting for cancer researchers considering that these natural materials could be suitable sources for finding new anti-cancer agents. In the present study, Acroptilon repens methanol extract had been evaluated for its cytotoxic effects in two human breast cancer cell lines, MCF-7 and MDA-MB-468, using MTT assay. The apoptosis potential had also been evaluated using annexin-V/propidium iodide assay, Hoechst 33258 staining and evaluating the cell cycle with flow cytometery. The MTT results showed cytotoxicity with IC50 values of 69.2 and 32.6 μg/mL for MCF-7 and MDA-MB-468 cells, respectively. The results of the apoptosis assays confirmed the apoptosis potential of the plant extract in the breast cancer cell lines suggesting A. repens for further cancer studies.

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“…It is widespread in Asia, North America, and Europe and predominates the arid regions of North Africa. Artemisia campestris has many medicinal actions, including: antivenom [10,11], anthelmintic [12], antimicrobial, antifungal, anticancer [13], antidiabetic [14,15,16], antihypertensive. It has been utilized in treating many conditions, including: digestive, respiratory, cutaneous and genital diseases [17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening and Antibacterial Activity of Menthe Piperita, Artemisa Compestris L and Pelargonium Adoraitissimum Leaves Extracts from North Libya

Yarboa¹

2018

BSI

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Phytochemical screening of the menthe piperita, artemisa compestris L and pelargonium adoraitissimum leave extracts from north Libya revealed the presence of some bioactive components. Various chemical tests showed that the bioactive compounds are found in methanolic extracts of these plants such as flavonoid, alkaloids, resins, terpineol, volatile oils and tannin groups. Saponin is present in Pelargonium adoraitissimum while it is absent in Menthe piperit and Artemisa compestris. The effects of methanolic extracts on some pathogenic: two strains of gram negative bacteria [Escherichia coli and Klebsiella pneumonia] and two strains of gram positive bacteria [Staphylococcus aureus and strrptococcus spp.] indiated that the menthe piperita has greater inhibition zone against two tested Gram negative bacteria and strains of gram positive bacteria.

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Cytotoxic activity evaluation of some medicinal plants, selected from Iranian traditional medicine Pharmacopoeia to treat cancer and related disorders

Cited by 31 publications

References 47 publications

Cytotoxic Activity and Apoptosis Induction of Hypericum scabrum L.

Cytotoxic Activity and Apoptosis Induction of Hypericum scabrum L.

Acroptilon repens induces apoptosis in human breast adenocarcinoma

Phytochemical Screening and Antibacterial Activity of Menthe Piperita, Artemisa Compestris L and Pelargonium Adoraitissimum Leaves Extracts from North Libya

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