Cytotoxic Activity and Antiproliferation of Soursop Seed (Annona muricata L.) Protein on MCF-7 Cells

Abstract

One of the new anticancer treatments that can be developed is a protein similar to RIPs. RIP is an enzyme found in various parts of plants that is toxic and can inhibit protein synthesis. Soursop seeds (Annona muricata L.) may be developed into anticancer. The plant of the same genus, Annona Squamosa L., has been proven to have a protein content similar to that of RIPs because it can supercoil DNA into circular nicks at low levels. Another study reported that soursop seed protein had moderate cytotoxic activity against 4T1 cells but did not show any antiproliferative activity. This study was conducted to determine the cytotoxic and antiproliferative activity of soursop seed (Annona muricata L.) protein against MCF-7 cells. Cytotoxic and antiproliferative activity tests were carried out using the MTT method on the protein fraction of soursop seeds. Cytotoxicity test was performed within 24 h. The antiproliferative test was carried out for 24, 48, and 72 h with the test parameter, doubling time. The results of the cytotoxicity test obtained that the protein fraction had weak cytotoxic activity with an IC 50 value of 429,90 μg /mL. The antiproliferative results implied that there was no growth inhibition as indicated by the concentration of the protein fractions of 7,5 μg/mL and 3,75 μg/mL, which were unable to prolong the time twice compared to control cells. The results of this study showed that the protein fractions of soursop seeds did not have antiproliferative activity against MCF-7 cells.