Cytotoxic Activities of 9,10-seco-Cycloartane-Type Triterpenoids from the Chinese Liverwort Lepidozia reptans

Abstract

Ten new triterpenoids, including nine 9,10-secocycloartanes (1−9) and one 9,19-cyclolanostane (10), as well as one sesquiterpenoid (11) and four known compounds (12−15), were extracted and purified from the whole plant of the Chinese liverwort Lepidozia reptans. Multiple techniques (NMR, HRESIMS, IR, and Xray crystallographic analysis) were applied to determine the structures of the isolated compounds. Bioassay determinations showed that compound 7, which contains an α,β-unsaturated carbonyl moiety in its structure, inhibited the growth of a panel of cancer cell lines with IC 50 values ranging from 4.2 ± 0.2 to 5.7 ± 0.5 μM. Further investigation revealed that compound 7 induces PC-3 cell death via mitochondrial-related apoptosis.

Interconvertible Pyridone Alkaloids from the Marine-Derived Fungus Penicillium oxalicum QDU1

“…Doxorubicin hydrochloride and cell culture medium were used as the positive and blank controls, respectively. The absorbance was measured at 450 nm with an enzyme labeling instrument, and the original data and results were recorded …”

“…The absorbance was measured at 450 nm with an enzyme labeling instrument, and the original data and results were recorded. 27 Test pathogens [Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Fusarium fetens, F. solanum, F. mangiferae, F. oxysporum, F. graminearum, Colletotrichum musae (ACCC 31244), C. coccodes (ACCC 36067), and Alternaria solani] were used to evaluate the antimicrobial activities of all compounds. Antimicrobial activity was carried out by the paper-agar disk diffusion assay.…”

New Compounds from Terrestrial Plants: A Perspective View

“…New triterpenic compounds were isolated from Lepidozia reptans (L.) Dumort. and named lepidozin A-J [96]. Lepidozin G (55) (Figure 12) is a 9,10-dry cycloartane that contains a carbonyl portion in an α,β-unsaturated portion in its structure that may be related to the inhibition of cancer cell lines by inducing the death of PC-3 cells by mitochondriarelated apoptosis.…”

New Compounds from Plants: A Perspective View

“…New triterpenic compounds were isolated from Lepidozia reptans (L.) Dumort. and named lepidozin A-J [96]. Lepidozin G ( 55) is a 9,10-dry cycloartane that contains a cabonyl portion in an α,β-unsaturated portion in its structure that may be related to the inhibition of cancer cell lines by inducing the death of PC-3 cells by mitochondria-related apoptosis.…”