Cytotherapy using stromal cells: Current and advance multi-treatment approaches

Shende, Pravin; Gupta, Hunny; Gaud, Ram

doi:10.1016/j.biopha.2017.10.127

Cited by 20 publications

(14 citation statements)

References 75 publications

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“…MSCs have the potential for multilineage differentiation (osteogenic, adipogenic and chondrogenic differentiation), and act as seed cells with broad applications [ 26 – 29 ]. MSCs account for only 0.001–0.01% of total bone marrow nucleated cells [ 30 ] and can be separated from the other cell types by taking advantage of their propensity to adhere to plastic surfaces.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells and porous β-tricalcium phosphate composites prepared through stem cell screen-enrich-combine(−biomaterials) circulating system for the repair of critical size bone defects in goat tibia

et al. 2018

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BackgroundEfficacious bone substitute is essential for the treatment of a critical size bone defect. Currently, the bone substitutes commonly used in clinical practice lack osteogenic capacity and the therapeutic efficacy is not ideal. Herein, a novel stem cell screen-enrich-combine(−biomaterials) circulating system (SECCS) was introduced to provide the substitutes with osteogenic ability. The stem cell screening, enrichment, and recombination with substitutes could be integrated during the surgical operation. Using SECCS, the bioactive mesenchymal stem cells (MSCs) and porous β-tricalcium phosphate (β-TCP) composites (MSCs/β-TCP) were rapidly prepared for critical size bone defect repair and validated in goat models of critical size tibia defects.MethodsTwelve goats with right hind limb tibia defects of 30 mm were randomly divided into two groups: six (the experimental group) were treated with MSCs/β-TCP prepared by SECCS and the other six goats (the control group) were treated with pure porous β-TCP. The repair effect was assessed by x-ray, computed tomography (CT), micro-CT, histology and histomorphology 6 months after the operation. In addition, the enrichment efficacy of MSCs and the characteristics of the MSCs/β-TCP prepared by SECCS were evaluated.ResultsThe SECCS could compound about 81.3 ± 3.0% of the MSCs in bone marrow to the porous β-TCP without affecting the cell viability. The average number of MSCs for retransplantation was 27,655.0 ± 5011.6 for each goat from the experimental group. In vitro, satisfactory biocompatibility of the MSCs/β-TCP was performed, with the MSCs spreading adequately, proliferating actively, and retaining the osteogenic potential. In vivo, the defect repair by MSCs/β-TCP with good medullary cavity recanalization and cortical remodeling was significantly superior to that of pure porous β-TCP.ConclusionsThe MSCs/β-TCP prepared through SECCS demonstrated significant therapeutic efficacy in goat models of critical size bone defect. This provides a novel therapeutic strategy for critical size bone defects caused by severe injury, infection, and bone tumor resection with a high profile of safety, effectiveness, simplicity, and ease.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells and porous β-tricalcium phosphate composites prepared through stem cell screen-enrich-combine(−biomaterials) circulating system for the repair of critical size bone defects in goat tibia

et al. 2018

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“…Most of the studies use stem cells for regenerative purposes. MSC are multipotent cells that can differentiate into adipocytes, osteoblasts, chondrocytes and neurons under appropriate conditions [68]. The main advantage of stem cell therapy is living biological replacement rather than palliation through drugs.…”

Section: Hypoxia Improves Differentiation Potential Of Cellsmentioning

confidence: 99%

Hypoxic Preconditioning as a Strategy to Maintain the Regenerative Potential of Mesenchymal Stem Cells

Bahir¹,

Choudhery²,

Hussain³

2020

Regenerative Medicine

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Mesenchymal stem cells (MSCs) are non-hematopoietic cells with high proliferative potential and multi-lineage differentiation capacity. MSCs are promising therapeutic candidates for cell-based therapies, and hundreds of clinical trials have been registered using these cells. Potential of stem cells is compromised with the factors such as disease condition and age of donor. Therefore, taking the cells from such patients for autologous use may compromise the benefits of cell-based therapies. It is therefore required to enhance the potential of these cells before use in stem cell-based therapies. Optimization of culture conditions is preferred strategies to enhance the regenerative potential of cells before use. This chapter briefly overviews the benefits of hypoxic preconditioning of stem cells to enhance the regenerative potential of cells in terms of their survival, proliferation, and differentiation.

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“…Stem cells have been classified by their capability to differentiate into various types of cells (by potency) and by their origin [11]. Totipotent cells (first cells from a zygote division) differentiate into all possible types of cells and organs.…”

Section: Cell Therapy For the Treatment Of Chronic Woundsmentioning

confidence: 99%

“…During the last two decades, exogenous SCs were administered in regenerative medicine to treat degenerative tissues and traumas with limited regenerative capacities. Therapeutic potential of SCs has three main mechanisms of action: (a) self-guidance, or the ability to migrate into the damaged area, following chemical gradients; (b) differentiation into various lineages of cells, which leads to a renewed functionality of damaged tissues; (c) a secretion of bioactive factors that can potentially impact both local and systemic physiological processes [11] ( Fig. 2).…”

Section: Cell Therapy For the Treatment Of Chronic Woundsmentioning

confidence: 99%

“…Epidermal, dermal cells and cells of hair follicles, as well as circulating in the bloodstream bone marrow mesenchymal stem cells, migrate to the damaged skin and take part in its regeneration [21]. Stem cell therapeutic efficacy in cutaneous wound healing is associated with the ability to migrate into the damaged area, following chemical gradients (a); differentiation into various lineages of cells, which leads to a renewed functionality of damaged tissues (b); a secretion of paracrine factors (c) [11].…”

Section: Figmentioning

confidence: 99%

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Regenerative therapy for the nonhealing cutaneous wounds

et al. 2018

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Regenerative medicine therapy is inspired by current research advances in cellular biology, genetic engineering, synthetic biology, material sciences and so far contributes to the traditional therapy, making resistant diseases curable. Nowadays, chronic wound healing is possible due to cell-based regenerative technologies and recent non-cell therapeutic approaches. Here we review clinical applications of human stem cells, as well as cellular and tissue products as alternatives to the traditional therapy of non-healing wounds. The cell-based technologies for tissue regeneration and bioengineering utilize stem cells that are either injected into bloodstream or positioned directly into the target area. Cell-free regeneration technologies require either stem cell products, i.e., secretomes or their separate components, extracellular membrane vesicles, or tissue products. The stem cell therapies are designed to replace critically absent components of wounded or degenerative tissue. The stem cell secretome can promote the repair of damaged tissues independently of parent cells. Extracellular membrane vesicles mimic and recapitulate the mechanisms of stem cells in tissue regeneration and therefore might be promising for chronic wound and severe burns healing. The tissue products traditionally remain efficient wound healing remedies along with emerging advanced technologies. K e y w o r d s: wound healing, cell and cell-free technologies, stem cells, secretomes, extracellular membrane vesicles, tissue products

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Cytotherapy using stromal cells: Current and advance multi-treatment approaches

Cited by 20 publications

References 75 publications

Mesenchymal stem cells and porous β-tricalcium phosphate composites prepared through stem cell screen-enrich-combine(−biomaterials) circulating system for the repair of critical size bone defects in goat tibia

Mesenchymal stem cells and porous β-tricalcium phosphate composites prepared through stem cell screen-enrich-combine(−biomaterials) circulating system for the repair of critical size bone defects in goat tibia

Hypoxic Preconditioning as a Strategy to Maintain the Regenerative Potential of Mesenchymal Stem Cells

Regenerative therapy for the nonhealing cutaneous wounds

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