Cytotaxonomic studies in<i>Vigna.</i>III. Chromosomal distribution and reacting properties of the heterochromatin in five wild species of the section<i>Vigna</i>

Galasso, Incoronata; Saponetti, Luisa Sublimi; Pignone, Domenico

doi:10.1080/00087114.1996.10797375

Cited by 18 publications

(11 citation statements)

References 9 publications

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“…The numbers of bands and the heterochromatin amount varied, but the general patterns were relatively conserved at the generic level (Galasso et al, 1996;Moscone et al, 1996;Miranda et al, 1997;Ran et al, 1999;Marcon and Guerra, 2005;Fregonezi et al, 2006). There is a positive association between the ploidy level and the amount of heterochromatin in the cytotypes of Maihuenia patagonica .…”

Section: Discussionmentioning

confidence: 96%

Karyotypes, heterochromatin, and physical mapping of 18S-26S rDNA in Cactaceae

Peñas

Urdampilleta

Bernardello

et al. 2009

Cytogenet Genome Res

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Karyotype analyses in members of the four Cactaceae subfamilies were performed. Numbers and karyotype formula obtained were: Pereskioideae = Pereskiaaculeata(2n = 22; 10 m + 1 sm), Maihuenioideae = Maihuenia patagonica (2n = 22, 9 m + 2 sm; 2n = 44, 18 m + 4 sm), Opuntioideae = Cumulopuntia recurvata(2n = 44; 20 m + 2 sm), Cactoideae = Acanthocalycium spiniflorum (2n = 22; 10 m + 1 sm),Echinopsis tubiflora (2n = 22; 10 m + 1 sm), Trichocereus candicans (2n = 22, 22 m). Chromosomes were small, the average chromosome length was 2.3 μm. Diploid species and the tetraploid C. recurvata had one terminal satellite, whereas the remaining tetraploid species showed four satellited chromosomes. Karyotypes were symmetrical. No CMA^–/DAPI⁺ bands were detected, but CMA⁺/DAPI^– bands associated with NOR were always found. Pericentromeric heterochromatin was found in C. recurvata, A. spiniflorum, and the tetraploid cytotype of M. patagonica. The locations of the 18S-26S rDNA sites in all species coincided with CMA⁺/DAPI^– bands; the same occurred with the sizes and numbers of signals for each species. This technique was applied for the first time in metaphase chromosomes in cacti. NOR-bearing pair no.1 may be homeologous in all species examined. In Cactaceae, the 18S-26S loci seem to be highly conserved.

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Section: Discussionmentioning

confidence: 96%

Karyotypes, heterochromatin, and physical mapping of 18S-26S rDNA in Cactaceae

Peñas

Urdampilleta

Bernardello

et al. 2009

Cytogenet Genome Res

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“…In most of the genera in which data were available for at least five species, the number of bands and the HC amount varied but the general pattern was relatively well conserved, as in Anacyclus (Schweizer and Ehrendorfer, 1976), Secale (Bennett et al, 1977), Vigna (Zheng et al, 1991;Galasso et al, 1996b) and Citrus (Guerra, 1993a;Miranda et al, 1997). The diversity was much higher in genera with karyotypes exhibiting numerous interstitial bands, like Anemone (Marks and Schweizer, 1974), Scilla (Greilhuber, 1982), and Tulipa (Blakey and Vosa, 1982).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, non-fluorescent DNA-ligands, like dystamycin A, may also be used to intensify the CMA or DAPI staining (Schweizer, 1983;Fuchs et al, 1998). However, not all AT-rich or GC-rich HC reacts equally to these fluorochromes (see e.g., Schwarzacher and Schweizer, 1982;Kenton, 1991;Bennett et al, 1995) and some C-banded positive regions react neutrally with fluorochromes, i.e., they fluoresce with the same brightness as euchromatin (Morawetz, 1986a,b;Röser, 1994;Galasso et al, 1996b;Cuellar et al, 1996). Other C-bands, fluoresce with the same intensity when stained with fluorochromes with different base-specificities, such as CMA and DAPI (Loidl, 1983;Guerra, 1989;Okada, 1991;Berg and Greilhuber, 1993).…”

Section: Heterochromatin Characterizationmentioning

confidence: 99%

Patterns of heterochromatin distribution in plant chromosomes

2000

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The C-band distribution patterns of 105 angiosperm species were compared to identify general patterns or preferential sites for heterochromatin. The base-specific fluorochrome reaction of heterochromatin for 58 of these species and the role played by the average chromosome size in band distribution were also considered. The results showed that heterochromatin was preferentially located in similar chromosome regions, regardless of the distance from the centromere. This trend results in generalized bands, with heterochromatin distribution being identical in most chromosomes of a karyotype. Such bands very often displayed the same fluorochrome reaction, suggesting possible repeat transfer between non-homologous sites. Chromosome size may also play a role in heterochromatin location, since proximal bands were much more common in small-sized chromosomes.

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“…In most genera in which data are available for at least five species, the numbers of bands and the heterochromatin amount varied, but the general patterns were relatively conserved [e.g. Vigna (Fabaceae; Galasso et al 1996), Citrus (Rutaceae; Miranda et al 1997), Clivia (Amaryllidaceae; Ran et al 1999), Cestrum (Solanaceae; Fregonezi et al 2006)]. In Pyrrhocactus species, terminal CMA + /DAPI -bands were observed, revealing GC-blocks in chromosome pair #1 that are associated with the NORs regions, a fact suggesting that they might be restricted to this location in the genus.…”

Section: Discussionmentioning

confidence: 99%

Karyotypes and fluorescent chromosome banding in Pyrrhocactus (Cactaceae)

2008

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Summary. Karyotypes of the seven Pyrrhocactus species were studied for the first time with Feulgen staining and CMA/DAPI banding. All showed 2n = 22 (x = 11). The karyotypes were symmetrical with 9m + 2sm pairs, excepting P. catamarcensis with 8m + 2sm + 1st pairs. They had a terminal microsatellite on short arms of pair #1. Pyrrhocactus bulbocalyx possessed a second satellited pair (#2) exclusively detected with Feulgen. Increasing asymmetry was associated with a decline in karyotype size. Fluorochrome banding, applied for the first time in Cactaceae, revealed that nucleolar chromosome pair #1 had one CMA + /DAPI -terminal band in all species related to the nucleolar organizing region; additional pericentromeric bands were found. A pattern of homogeneous sized chromosomes with median and submedian centromeres is conserved in the genus. However, karyotypes can be distinguished by a combination of cytogenetic features. Species diversification in Pyrrhocactus has not been associated with large chromosome rearrangements or polyploidy, but with cumulative small and cryptic structural changes.

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Cytotaxonomic studies inVigna.III. Chromosomal distribution and reacting properties of the heterochromatin in five wild species of the sectionVigna

Cited by 18 publications

References 9 publications

Karyotypes, heterochromatin, and physical mapping of 18S-26S rDNA in Cactaceae

Karyotypes, heterochromatin, and physical mapping of 18S-26S rDNA in Cactaceae

Patterns of heterochromatin distribution in plant chromosomes

Karyotypes and fluorescent chromosome banding in Pyrrhocactus (Cactaceae)

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