Cytosymbiosis

A eukaryotic genome of 660 kb: electrophoretic karyotype of nucleomorph and cell nucleus of the cryptomonad alga,Pyrenomonas salina

“…Buchler, Braunschweig, FRG) after UV nicking (20). The probes On account of the earlier finding (22) that plastidal and were prepared by the random hexamer priming method (21) using mitochondrial DNAs of P. salina are circular molecules of about Fractionation of chromosome-sized DNA molecules from P. salina is shown in Fig. la.…”

“…These three chromosomes with sizes of 195 kb, 225 kb, and 240 be separated from total cells of P. salina ( Fig.2) [3][4][5][6]. DNAs for the homologous probes were isolated as described previously (16,22). The rDNA probes were fragments of maize nuclear rRNA-genes (25).…”

Chloroplast protein and centrosomal genes, a tRNA intron, and odd telomeres in an unusually compact eukaryotic genome, the cryptomonad nucleomorph

“…The presence of histone genes shows that, even though the nucleomorph is highly miniaturized compared with a typical nucleus, its chromatin probably has the same basic organization; previous attempts to demonstrate histones in nucleomorphs were inconclusive (22)(23)(24). The presence of ␣-and ␤-tubulin genes, despite the absence of microscopically detectable microtubules during nucleomorph division (25,26), suggests that this dwarfed nucleus may have some very short and transient microtubules and a form of mitosis, and thus be a paradigm in miniature for understanding chromosome segregation in ordinary nuclei.…”