Cytostatic Elimination of Syngeneic Rat Tumor Cells in Vitro by Nonspecifically Activated Macrophages

Abstract

Methyleholanthrene tumors: Methylcholanthrene tumors were obtained after instillation

Natural cytotoxic macrophages in the peritoneal cavity of mice

“…(Meltzer, 1976: Li et al, 1977Kefler, 1978a), nude mice (Meltzer, 1976) and rats (Keller, 1978a, b Keller, 1973;Krahenbuhl & Remington, 1974;Pels & Den Otter, 1974;Fidler, 1975;Krahenbuhl & Lambert, 1975;Fidler et al, 1976;Krahenbuhl et al, 1976 Li et al (1977). However, the natural cytotoxicity itself seemed not to be due to factors in the serum as (a) all the different sera tested, (b) Rehatuin which should be free of endotoxin, (e) fractionated, and (d) adsorbed serum gave comparable results.…”

Comparison of three isotope-release assays for spontaneous cytotoxicity of macrophages

“…Early passages of DA rat embryonic fibroblasts (ERF; Keller, 1976a) DA rat DMBA-induced fibrosarcoma cells growing as ascites tumour (DMBA-12;Keller, 1977) DA rat polyoma-induced tumour cells (Py-12;Keller, 1973) DBA/2 murine mastocytoma P-815 cells (Keller, 1976a) SV40-transformed C57BL mouse macrophages (IC-21-B4; Keller, 1978) RPMI 7932 human melanoma cells (RPMI; Keller, 1976a) and the Burkitt's lymphoma cell line RAJI (Keller, 1976a) were obtained as previously described. The BALB/c-derived monocyte cell line, PU5-1 -8 (Ralph et al, 1977) was a gift from Dr P. Ralph.…”

Macrophage-mediated natural cytotoxicity against various target cells in vitro. II. Macrophages from rats of different ages

“…As effector cells from normal tissues are far more heterogeneous than peritonealexudate cells (Keller, 1976b), a role for effector cells other than mononuclear phagocytes capable of mediating spontaneous cytotoxicity also merits consideration. In view of the recently recognized diversity of the cell populations manifesting natural killer capacity, the demonstration of consistent abrogation of the cytolytic effector-cell activity in vitro by silica (Keller, 1973(Keller, , 1978, an agent accepted as selectively toxic for macrophages (Kessel, Monaco and Marchisio, 1963;Allison, Harington and Birbeck, 1966;Keller, 1976a) may not be entirely conclusive. It is therefore essential clearly to identify and characterize the mononuclear phagocytes from other cell types manifesting spontaneous killer capacity.…”

“…Target cells.-Apart from target cell types used in previous work (Keller, 1978), polyomavirus-induced tumour cells from DA rats (Keller, 1973;Keller and Keist, 1978) were also included in this study. All targets were grown in RPMI-1640 medium supplemented with 10% foetal calf serum (FCS; Gibco, Grand Island, N.Y.).…”