Cytostatic effects of horseradish and thyroid peroxidase derived free radicals

Using a procedure aimed at isolation of genes that are inactivated during nickel-induced carcinogenesis in Chinese hamster cells, a homolog of genes encoding human and mouse heme containing peroxidases has been cloned. Northern blot analysis of normal cultured fibroblasts and two nickel-transformed cell lines confirmed that this gene was expressed in normal but not in transformed cells. Nickel-transformed cells also tested negative for peroxidase activity using a sensitive fluorescence assay. Cultured embryo cells or fibroblasts that express peroxidase activity and their nickel-transformed peroxidase-deficient counterparts were employed to investigate the role of peroxidase-catalyzed processes in cytotoxicity induced by tert-butyl hydroperoxide or cumene hydroperoxide. It has been found that peroxidase-deficient cells were significantly more resistant to cytotoxic effect of these compounds suggesting that cytotoxic effect of hydroperoxides may be mediated in part by free radicals generated in the course of peroxidase-catalyzed reactions.

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Peroxidase deficiency of nickel-transformed hamster cells correlates with their increased resistance to cytotoxicity of peroxides

Dowjat

Huang

Cosentino

et al. 1996

Biometals

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Free radicals and pathogenesis during ischemia and reperfusion of the cat small intestine

et al. 1994

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Iodine-induced thyroid inhibition and cell necrosis: two consequences of the same free-radical mediated mechanism?

Denef¹,

Many²,

Hove

1996

Molecular and Cellular Endocrinology

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Cytostatic effects of horseradish and thyroid peroxidase derived free radicals

Cited by 4 publications

References 33 publications

Peroxidase deficiency of nickel-transformed hamster cells correlates with their increased resistance to cytotoxicity of peroxides

Peroxidase deficiency of nickel-transformed hamster cells correlates with their increased resistance to cytotoxicity of peroxides

Free radicals and pathogenesis during ischemia and reperfusion of the cat small intestine

Iodine-induced thyroid inhibition and cell necrosis: two consequences of the same free-radical mediated mechanism?

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