Cytostatic and genotoxic effect of temephos in human lymphocytes and HepG2 cells

Benítez-Trinidad, Alma Betsaida; Herrera-Moreno, José Francisco; Vázquez-Estrada, G.; Verdín-Betancourt, Francisco Alberto; Sordo, Monserrat; Ostrosky‐Wegman, Patricia; Bernal-Hernández, Yael Yvette; Medina-Díaz, Irma Martha; Barrón-Vivanco, Briscia Socorro; Robledo-Marenco, M.L.; Salazar, Ana María; Rojas-García, Aurora Elizabeth

doi:10.1016/j.tiv.2015.02.008

Cited by 33 publications

(20 citation statements)

References 24 publications

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“…Exposure to environmental toxicants has also been shown to induce higher gentoxic alterations. The use of common organophosphorus pesticides is linked to elevated DNA damage in human lymphocytes, and both DNA damage and micronucleus frequency in liver hepatocellular carcinoma (HepG2) cells (118). A comprehensive review on the genotoxic effect of commonly used pesticides by persons in the work place and those employed in the chemical industry was conducted by Bolognesi et al (5), highlighting the importance of genotoxic biomonitoring in human populations who might be at a greater risk of cancer.…”

Section: Environmental Toxins and Chromatin Instabilitymentioning

confidence: 99%

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Hernández-Saavedra

Strakovsky

Ostrosky‐Wegman

et al. 2017

Advances in Nutrition

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The centromere is a genomic locus required for the segregation of the chromosomes during cell division. This chromosomal region together with pericentromeres has been found to be susceptible to damage, and thus the perturbation of the centromere could lead to the development of aneuploidic events. Metabolic abnormalities that underlie the generation of cancer include inflammation, oxidative stress, cell cycle deregulation, and numerous others. The micronucleus assay, an early clinical marker of cancer, has been shown to provide a reliable measure of genotoxic damage that may signal cancer initiation. In the current review, we will discuss the events that lead to micronucleus formation and centromeric and pericentromeric chromatin instability, as well transcripts emanating from these regions, which were previously thought to be inactive. Studies were selected in PubMed if they reported the effects of nutritional status (macro- and micronutrients) or environmental toxicant exposure on micronucleus frequency or any other chromosomal abnormality in humans, animals, or cell models. Mounting evidence from epidemiologic, environmental, and nutritional studies provides a novel perspective on the origination of aneuploidic events. Although substantial evidence exists describing the role that nutritional status and environmental toxicants have on the generation of micronuclei and other nuclear aberrations, limited information is available to describe the importance of macro- and micronutrients on centromeric and pericentromeric chromatin stability. Moving forward, studies that specifically address the direct link between nutritional status, excess, or deficiency and the epigenetic regulation of the centromere will provide much needed insight into the nutritional and environmental regulation of this chromosomal region and the initiation of aneuploidy.

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Section: Environmental Toxins and Chromatin Instabilitymentioning

confidence: 99%

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Hernández-Saavedra

Strakovsky

Ostrosky‐Wegman

et al. 2017

Advances in Nutrition

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“…Studies have demonstrated that temephos has cytostatic and genotoxic effects on human cells in vitro [16, 17]. A small study in India of 70 workers occupationally exposed to a mixture of pesticides, including temephos, reported evidence of DNA damage, decreased anti-cholinesterase activity and abnormal liver enzymes [18].…”

Section: Introductionmentioning

confidence: 99%

Coverage and beliefs about temephos application for control of dengue vectors and impact of a community-based prevention intervention: secondary analysis from the Camino Verde trial in Mexico

et al. 2017

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BackgroundTemephos in domestic water containers remains a mainstay of Latin American government programmes for control of Aedes aegypti and associated illnesses, including dengue. There is little published evidence about coverage of routine temephos programmes. A cluster randomised controlled trial of community mobilisation in Mexico and Nicaragua reduced vector indices, dengue infection, and clinical dengue cases. Secondary analysis from the Mexican arm of the trial examined temephos coverage and beliefs, and the impact of the trial on these outcomes.MethodsThe trial impact survey in December 2012, in 10,491 households in 45 intervention and 45 control clusters, asked about visits from the temephos programme, retention of applied temephos, and views about temephos and mosquito control. Fieldworkers noted if temephos was present in water containers.ResultsSome 42.4% of rural and 20.7% of urban households reported no temephos programme visits within the last 12 months. Overall, 42.0% reported they had temephos placed in their water containers less than 3 months previously. Fieldworkers observed temephos in at least one container in 21.1% of households. Recent temephos application and observed temephos were both significantly more common in urban households, when other household variables were taken into account; in rural areas, smaller households were more likely to have temephos present.Most households (74.4%) did not think bathing with water containing temephos carried any health risk. Half (51%) believed drinking or cooking with such water could be harmful and 17.6% were unsure.Significantly fewer households in intervention sites (16.5%) than in control sites (26.0%) (Risk Difference − 0.095, 95% confidence interval − 0.182 to −0.009) had temephos observed in their water; more households in intervention clusters (41.8%) than in control clusters (31.6%) removed the applied temephos quickly. Although fewer households in intervention sites (82.7%) compared with control sites (86.7%) (RD -0.04, 95% CI -0.067 to −0.013) agreed temephos and fumigation was the best way to avoid mosquitoes, the proportion believing this remained very high.ConclusionCoverage with the government temephos programme was low, especially in rural areas. Despite an intervention encouraging non-chemical mosquito control, most households continued to believe that chemicals are the best control method.Trial registrationISRCTN:27581154.Electronic supplementary materialThe online version of this article (doi:10.1186/s12889-017-4297-5) contains supplementary material, which is available to authorized users.

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“…Genetic injury is the chronic toxic effect of organophosphorus pesticide on the exposure- crowd. The studies in this area are mainly involving in the comet assay and micronucleus test [ 24 – 27 ]. However, genetic injury cannot occur in all individuals who have the same contact condition, suggesting that omethoate-exposure population have different genetic susceptibility.…”

Section: Discussionmentioning

confidence: 99%

Cross-sectional associations between genetic polymorphisms in metabolic enzymes and longer leukocyte telomere length induced by omethoate

et al. 2017

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PurposeThis study aimed to explore the effects of genetic polymorphisms in metabolic enzymes on relative telomere length changes and explore the mechanism of canceration induced by omethoate.Materials and Methods180 long-term omethoate-exposed workers and 115 healthy controls were recruited. Real-time PCR method was applied to determine the relative telomere length in peripheral blood leukocytes DNA, and Six polymorphic loci of GSTT1(+/−), GSTM1(+/−), GSTP1 rs1695, CYP2E1 rs6413432, CYP2E1 rs3813867 and PON2 rs12026 were detected by polymerase chain reaction and restriction fragment length polymorphism method; Multiple linear regression was conducted to explore the effects of omethoate exposure and genetic polymorphisms on the telomere length.ResultsThe relative telomere lengths in the control group (0.94 [0.76, 1.32]) were significantly shorter than that in the exposure group (1.50 [1.11, 2.57]) (Z = 7.910, P < 0.001). Univariate analysis showed that the relative telomere lengths of the GSTM1-deletion individuals were significantly longer than that of the non - deletion genotype in the control group (Z = 2.911, P = 0.004), and the relative telomere lengths of GSTP1 rs1695 polymorphism locus (GG+AG) genotype individuals were longer than that of AA genotype in the exposure group. The difference was statistically significant (Z = 2.262, P = 0.024). Multivariate analysis found that pesticide-exposure (b = 0.524, P < 0.001) and GSTM1 polymorphism (b = −0.136, P = 0.029) had an impact on telomere length.ConclusionsThe relative telomere lengths of omethoate-exposure workers were longer than that in the control population. Also GSTM1 genetic polymorphism may influence the changes of the telomere length induced by omethoate.

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Cytostatic and genotoxic effect of temephos in human lymphocytes and HepG2 cells

Cited by 33 publications

References 24 publications

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Coverage and beliefs about temephos application for control of dengue vectors and impact of a community-based prevention intervention: secondary analysis from the Camino Verde trial in Mexico

Cross-sectional associations between genetic polymorphisms in metabolic enzymes and longer leukocyte telomere length induced by omethoate

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