Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots

Leterrier, Marina; Barroso, Juan B.; Palma, José M.; Corpas, Francisco J.

doi:10.1007/s10535-012-0244-6

Cited by 28 publications

(15 citation statements)

References 39 publications

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“…More specifically, the decreased accumulation of glycolate oxidase, a peroxisomal FMN-depended oxidase that catalyzes the oxidation of a-hydroxy acids to a-ketoacids, indicates that photorespiration is suppressed under SNP plus PEG conditions (Xu et al 2011). The observed decrease in abundance of NADP-isocitrate dehydrogenase could be attributed to a metabolic interconnection of this enzyme with molecules involved in RNS metabolism, as already proposed by Leterrier et al (2012). The increased abundance of UPF0603 protein Atlg54780, which is involved in photosystem II repair cycle, could be associated with the elevated level of chlorophyll and photosynthetic rate (Fig.…”

Section: Discussionsupporting

confidence: 66%

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Ziogas

Tanou

Belghazi³

et al. 2015

Plant Mol Biol

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Emerging evidence suggests that the gaseous molecules hydrogen sulfide (H2S) and nitric oxide (NO) enhances plant acclimation to stress; however, the underlying mechanism remains unclear. In this work, we explored if pretreatment of citrus roots with NaHS (a H2S donor) or sodium nitroprusside (SNP, a NO donor) for 2 days (d) could elicit long-lasting priming effects to subsequent exposure to PEG-associated drought stress for 21 d following a 5 d acclimation period. Detailed physiological study documented that both pretreatments primed plants against drought stress. Analysis of the level of nitrite, NOx, S-nitrosoglutahione reductase, Tyr-nitration and S-nitrosylation along with the expression of genes involved in NO-generation suggested that the nitrosative status of leaves and roots was altered by NaHS and SNP. Using a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemical treatments, including well known and novel S-nitrosylated targets. Mass spectrometry analysis also enabled the identification of 42 differentially expressed proteins in PEG alone-treated plants. Several PEG-responsive proteins were down-regulated, especially photosynthetic proteins. Finally, the identification of specific proteins that were regulated by NaHS and SNP under PEG conditions provides novel insight into long-term drought priming in plants and in a fruit crop such as citrus in particular.

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Section: Discussionsupporting

confidence: 66%

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Ziogas

Tanou

Belghazi³

et al. 2015

Plant Mol Biol

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“…Both are NADPH-generating dehydrogenases in plants, and NADPH is a key cofactor in the cellular redox homeostasis and necessary in the metabolism of ROS [ 66 ]. In Arabidopsis thaliana , NADP-ICDH activity is regulated by molecules involved in ROS, including H 2 O 2 [ 67 ]. These two types of miRNAs had no significant differences in expression levels between the NJCMS1A and NJCMS1B.…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNAs and their targets by high-throughput sequencing and degradome analysis in cytoplasmic male-sterile line NJCMS1A and its maintainer NJCMS1B of soybean

Ding

Zhang

et al. 2016

BMC Genomics

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BackgroundCytoplasmic male sterility (CMS) provides crucial breeding materials that facilitate hybrid seed production in various crops, and thus plays an important role in the study of hybrid vigor (heterosis), in plants. However, the CMS regulatory network in soybean remains unclear. MicroRNAs (miRNAs) play crucial roles in flower and pollen development by targeting genes that regulate their expression in plants. To identify the miRNAs and their targets that exist in the soybean CMS line NJCMS1A and its maintainer NJCMS1B, high-throughput sequencing and degradome analysis were conducted in this study.ResultsTwo small RNA libraries were constructed from the flower buds of the soybean CMS line NJCMS1A and its maintainer NJCMS1B. A total of 105 new miRNAs present on the other arm of known pre-miRNAs, 23 new miRNA members, 158 novel miRNAs and 160 high-confidence soybean miRNAs were identified using high-throughput sequencing. Among the identified miRNAs, 101 differentially expressed miRNAs with greater than two-fold changes between NJCMS1A and NJCMS1B were discovered. The different expression levels of selected miRNAs were confirmed by stem-loop quantitative real-time PCR. A degradome analysis showed that 856 targets were predicted to be targeted by 296 miRNAs, including a squamosa promoter-binding protein-like transcription factor family protein, a pentatricopeptide repeat-containing protein, and an auxin response factor, which were previously shown to be involved in floral organ or anther development in plants. Additionally, some targets, including a MADS-box transcription factor, NADP-dependent isocitrate dehydrogenase and NADH-ubiquinone oxidoreductase 24 kDa subunit, were identified, and they may have some relationship with the programmed cell death, reactive oxygen species accumulation and energy deficiencies, which might lead to soybean male sterility.ConclusionsThe present study is the first to use deep sequencing technology to identify miRNAs and their targets in the flower buds of the soybean CMS line NJCMS1A and its maintainer NJCMS1B. The results revealed that the miRNAs might participate in flower and pollen development, which could facilitate our understanding of the molecular mechanisms behind CMS in soybean.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2352-0) contains supplementary material, which is available to authorized users.

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“…The presence of NADP-ICDH in roots has been described in many plant species including pea plants (Chen et al , 1988), alfalfa (Shorrosh and Dixon, 1992), soybean (Udvardi et al , 1993), pines (Palomo et al , 1998; Pascual et al , 2008), and Arabidopsis (Leterrier et al , 2012b) and it has always been associated with the metabolism of amino acids and ammonia (Fieuw et al , 1995; Gálvez and Gadal, 1995). However, the concomitant generation of NADPH opens up new avenues in the functioning of this enzyme given that this cofactor is necessary for different metabolic pathways including the ascorbate–glutathione cycle, the superoxide radical generated by NADPH oxidase, and NO generation through l -arginine-dependent NOS activity.…”

Section: Discussionmentioning

confidence: 99%

Protein tyrosine nitration in pea roots during development and senescence

Begara-Morales

Chaki

Sánchez-Calvo

et al. 2013

Journal of Experimental Botany

162

105

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Protein tyrosine nitration is a post-translational modification mediated by reactive nitrogen species (RNS) that is associated with nitro-oxidative damage. No information about this process is available in relation to higher plants during development and senescence. Using pea plants at different developmental stages (ranging from 8 to 71 days), tyrosine nitration in the main organs (roots, stems, leaves, flowers, and fruits) was analysed using immunological and proteomic approaches. In the roots of 71-day-old senescent plants, nitroproteome analysis enabled the identification a total of 16 nitrotyrosine-immunopositive proteins. Among the proteins identified, NADP-isocitrate dehydrogenase (ICDH), an enzyme involved in the carbon and nitrogen metabolism, redox regulation, and responses to oxidative stress, was selected to evaluate the effect of nitration. NADP-ICDH activity fell by 75% during senescence. Analysis showed that peroxynitrite inhibits recombinant cytosolic NADP-ICDH activity through a process of nitration. Of the 12 tyrosines present in this enzyme, mass spectrometric analysis of nitrated recombinant cytosolic NADP-ICDH enabled this study to identify the Tyr392 as exclusively nitrated by peroxynitrite. The data as a whole reveal that protein tyrosine nitration is a nitric oxide-derived PTM prevalent throughout root development and intensifies during senescence.

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Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots

Cited by 28 publications

References 39 publications

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Identification of miRNAs and their targets by high-throughput sequencing and degradome analysis in cytoplasmic male-sterile line NJCMS1A and its maintainer NJCMS1B of soybean

Protein tyrosine nitration in pea roots during development and senescence

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