Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase Is Phosphorylated during Seed Development

Piattoni, Claudia Vanesa; Ferrero, Danisa María Luján; Dellaferrera, Ignacio; Vegetti, Abelardo Carlos; Iglesias, Alberto A.

doi:10.3389/fpls.2017.00522

Cited by 34 publications

(28 citation statements)

References 64 publications

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“…For example, S -glutathionylation of TRX f in chloroplast could alter its oxido-reductase activity toward targets, affecting them indirectly (Michelet et al, 2005). The SnRK1 is an essential regulator of plant metabolism by direct phosphorylation of a number of enzymes involved in carbohydrate metabolism and nitrogen assimilation (Piattoni et al, 2017; Wurzinger et al, 2018). Recently, it was determined that A. thaliana SnRK1 kinase activity is sensitive to inhibition by H 2 O 2 .…”

Section: Effects Of Redox Modifications Of Glycolytic Fermentative Amentioning

confidence: 99%

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

2019

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Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are present at low and controlled levels under normal conditions. These reactive molecules can increase to high levels under various biotic and abiotic conditions, resulting in perturbation of the cellular redox state that can ultimately lead to oxidative or nitrosative stress. In this review, we analyze the various effects that result from alterations of redox homeostasis on plant glycolytic pathway and tricarboxylic acid (TCA) cycle. Most documented modifications caused by ROS or RNS are due to the presence of redox-sensitive cysteine thiol groups in proteins. Redox modifications include Cys oxidation, disulfide bond formation, S -glutathionylation, S -nitrosylation, and S -sulfhydration. A growing number of proteomic surveys and biochemical studies document the occurrence of ROS- or RNS-mediated modification in enzymes of glycolysis and the TCA cycle. In a few cases, these modifications have been shown to affect enzyme activity, suggesting an operational regulatory mechanism in vivo . Further changes induced by oxidative stress conditions include the proposed redox-dependent modifications in the subcellular distribution of a putative redox sensor, NAD-glyceraldehyde-3P dehydrogenase and the micro-compartmentation of cytosolic glycolytic enzymes. Data from the literature indicate that oxidative stress may induce complex changes in metabolite pools in central carbon metabolism. This information is discussed in the context of our understanding of plant metabolic response to oxidative stress.

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Section: Effects Of Redox Modifications Of Glycolytic Fermentative Amentioning

confidence: 99%

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

2019

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“…Phosphorylated α-GP is active, and dephosphorylated α-GP is inactive [43]. GAPDH catalyzes a critical step in glycolysis; GAPDH activity was significantly decreased if phosphorylated [44]. In this study, phosphorylated α-GP was up-regulated both in CO39 and C101LAC 12 h after SA treatment, suggesting that SA might increase α-GP activity.…”

Section: Discussionmentioning

confidence: 70%

Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid

et al. 2019

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BackgroundSalicylic acid (SA) is a significant signaling molecule that induces rice resistance against pathogen invasion. Protein phosphorylation carries out an important regulatory function in plant defense responses, while the global phosphoproteome changes in rice response to SA-mediated defense response has not been reported. In this study, a comparative phosphoproteomic profiling was conducted by two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) analysis, with two near-isogenic rice cultivars after SA treatment.ResultsThirty-seven phosphoprotein spots were differentially expressed after SA treatment, twenty-nine of which were identified by MALDI-TOF/TOF MS, belonging to nine functional categories. Phosphoproteins involved in photosynthesis, antioxidative enzymes, molecular chaperones were similarly expressed in the two cultivars, suggesting SA might alleviate decreases in plant photosynthesis, regulate the antioxidant defense activities, thus improving basal resistance response in both cultivars. Meanwhile, phosphoproteins related to defense, carbohydrate metabolism, protein synthesis and degradation were differentially expressed, suggesting phosphorylation regulation mediated by SA may coordinate complex cellular activities in the two cultivars. Furthermore, the phosphorylation sites of four identified phosphoproteins were verified by NanoLC-MS/MS, and phosphorylated regulation of three enzymes (cinnamoyl-CoA reductase, phosphoglycerate mutase and ascorbate peroxidase) was validated by activity determination.ConclusionsOur study suggested that phosphorylation regulation mediated by SA may contribute to the different resistance response of the two cultivars. To our knowledge, this is the first report to measure rice phosphoproteomic changes in response to SA, which provides new insights into molecular mechanisms of SA-induced rice defense.

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“…Starch content was determined with the anthrone method [ 93 ] at 640 nm using soluble starch as standard. For protein analysis, 0.5 g of dry seeds were ground and extracted according to the method as described by Piattoni et al [ 94 ]. The total protein content was measured by using a spectrophotometer at 595 nm following the Bradford protocol [ 95 ].…”

Section: Methodsmentioning

confidence: 99%

Insights into the endophytic bacterial community comparison and their potential role in the dimorphic seeds of halophyte Suaeda glauca

et al. 2021

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Background Seed dimorphism has been thought to be a bet-hedging strategy that helps plants survive in the disturbed environment and has been widely studied for its ecological adaptation mechanism. Many studies showed that seed-associated microorganisms play an important role in enhancing plant fitness, but information regarding endophytic bacteria associated with dimorphic seeds is limited. This study explores the influence of seed coat structure and seed phytochemical properties on the community composition and diversity of endophytic bacteria of dimorphic seeds of Suaeda glauca. In this study, we used 16S rRNA high-throughput gene sequencing method to compare the community composition and bacterial diversity between brown and black seeds of Suaeda glauca. Results A significant difference was observed in seed coat structure and phytochemical properties between brown and black seeds of S. glauca. Total 9 phyla, 13 classes, 31 orders, 53 families, 102 genera were identified in the dimorphic seeds. The dominant phyla were Proteobacteria, Firmicutes, and Actinobacteria. The results showed that seed dimorphism had little impact on the diversity and richness of endophytic bacterial communities but significantly differs in the relative abundance of the bacterial community between brown and black seeds. At the phylum level, Actinobacteria tend to be enriched significantly in brown seeds. At the genus level, Rhodococcus, Ralstonia, Pelomonas and Bradyrhizobium tend to be enriched significantly in brown seeds, while Marinilactibacillus was mainly found in black seeds. Besides, brown seeds harbored a large number of bacteria with plant-growth-promoting traits, whereas black seeds presented bacteria with enzyme activities (i.e., pectinase, cellulolytic and xylanolytic activities). Conclusion The endophytic bacterial community compositions were significantly different between dimorphic seeds of Suaeda glauca, and play an important role in the ecological adaptation of dimorphic seeds by performing different biological function roles. The endophytic bacterial communities of the dimorphic seeds may be influenced mainly by the seed coat structureand partly by the seed phytochemical characteristics. These findings provide valuable information for better understanding of the ecological adaptation strategy of dimorphic seeds in the disturbed environment.

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Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase Is Phosphorylated during Seed Development

Cited by 34 publications

References 64 publications

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid

Insights into the endophytic bacterial community comparison and their potential role in the dimorphic seeds of halophyte Suaeda glauca

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