Cytosolic GABA inhibits anion transport by wheat ALMT1

Long, Yu; Tyerman, Stephen D.; Gilliham, Matthew

doi:10.1111/nph.16238

Cited by 28 publications

(32 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…170 However, GABA can inhibit anion transport by TaALMT1 from the inside and outside of a cell. 172,173 The molecular mechanism may resemble the conformational transition of GabR when binding to the pyridoxal 5′-phosphate (PLP)-dependent aspartate aminotransferase (AAT) and GABA. 174 It is possible that GABA causes the TaALMT1 active structure to make a conformational transition and render TaALMT1 unable to transport anions.…”

Section: Gaba Transporters In Plantsmentioning

confidence: 99%

See 1 more Smart Citation

The versatile GABA in plants

Dou

Zhang

et al. 2021

Plant Signaling & Behavior

140

View full text Add to dashboard Cite

Gamma-aminobutyric acid (GABA) is a ubiquitous four-carbon, non-protein amino acid. GABA has been widely studied in animal central nervous systems, where it acts as an inhibitory neurotransmitter. In plants, it is metabolized through the GABA shunt pathway, a bypass of the tricarboxylic acid (TCA) cycle. Additionally, it can be synthesized through the polyamine metabolic pathway. GABA acts as a signal in Agrobacterium tumefaciens -mediated plant gene transformation and in plant development, especially in pollen tube elongation (to enter the ovule), root growth, fruit ripening, and seed germination. It is accumulated during plant responses to environmental stresses and pathogen and insect attacks. A high concentration of GABA elevates plant stress tolerance by improving photosynthesis, inhibiting reactive oxygen species (ROS) generation, activating antioxidant enzymes, and regulating stomatal opening in drought stress. The transporters of GABA in plants are reviewed in this work. We summarize the recent research on GABA function and transporters with the goal of providing a review of GABA in plants.

show abstract

Section: Gaba Transporters In Plantsmentioning

confidence: 99%

“…174 It is possible that GABA causes the TaALMT1 active structure to make a conformational transition and render TaALMT1 unable to transport anions. 173…”

Section: Gaba Transporters In Plantsmentioning

confidence: 99%

The versatile GABA in plants

Dou

Zhang

et al. 2021

Plant Signaling & Behavior

140

View full text Add to dashboard Cite

show abstract

“…When GABA catabolism into the TCA cycle is reduced, this impacts the growth of the root systems and the structure of cell walls and sugar and starch catabolism increases (Renault et al, 2013). The GABA shunt is imperative both for plant development and in responses to various stresses and GABA can also act as a signalling molecule that interacts and modifies protein activity (Fait et al, 2008;Long et al, 2020).…”

Section: The Role Of the G-aminobutyric-acid Shunt During Salinitymentioning

confidence: 99%

Can Alternative Metabolic Pathways and Shunts Overcome Salinity Induced Inhibition of Central Carbon Metabolism in Crops?

Bandehagh

Taylor

2020

Front. Plant Sci.

View full text Add to dashboard Cite

“…Malate efflux through TaAMLT1 and other ALMTs was also found to be inhibited by micromolar concentrations of GABA, and its analogue muscimol, although in intact roots higher concentrations of GABA in the millimolar range were required for inhibition (Ramesh et al, 2015). Recently, it was found that the single channel currents corresponding to malate efflux through TaALMT1 are inhibited by GABA from the cytosolic face of the channel (Long, Tyerman, & Gilliham, 2020). Comparing the ET8 and ES8 NILs it was found that ET8 seedlings exuded more malate at pH 9 and that root growth of 3 to 4-day-old seedlings was higher in ET8 than ES8 at pH 9 (Ramesh et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Role of TaALMT1 malate‐GABA transporter in alkaline pH tolerance of wheat

Kamran

Ramesh

Gilliham

et al. 2020

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Malate exudation through wheat (Triticum aestivum L) aluminium‐activated malate transporter 1 (TaALMT1) confers Al3+ tolerance at low pH, but is also activated by alkaline pH, and is regulated by and facilitates significant transport of gamma‐aminobutyric acid (GABA, a zwitterionic buffer). Therefore, TaALMT1 may facilitate acidification of an alkaline rhizosphere by promoting exudation of both malate and GABA. Here, the performance of wheat near isogenic lines ET8 (Al+3‐tolerant, high TaALMT1 expression) and ES8 (Al+3‐sensitive, low TaALMT1 expression) are compared. Root growth (at 5 weeks) was higher for ET8 than ES8 at pH 9. ET8 roots exuded more malate and GABA at high pH and acidified the rhizosphere more rapidly. GABA and malate exudation was enhanced at high pH by the addition of aluminate in both ET8 and transgenic barley expressing TaALMT1. Xenopus laevis oocytes expressing TaALMT1 acidified an alkaline media more rapidly than controls corresponding to higher GABA efflux. TaALMT1 expression did not change under alkaline conditions but key genes involved in GABA turnover changed in accordance with a high rate of GABA synthesis. We propose that TaALMT1 plays a role in alkaline tolerance by exuding malate and GABA, possibly coupled to proton efflux.

show abstract

Cytosolic GABA inhibits anion transport by wheat ALMT1

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 28 publications

References 27 publications

The versatile GABA in plants

The versatile GABA in plants

Can Alternative Metabolic Pathways and Shunts Overcome Salinity Induced Inhibition of Central Carbon Metabolism in Crops?

Role of TaALMT1 malate‐GABA transporter in alkaline pH tolerance of wheat

Contact Info

Product

Resources

About