Cytosolic calcium rises and related events in ergosterol-treated Nicotiana cells

Vatsa, Parul; Chiltz, Annick; Luini, Estelle; Vandelle, Elodie; Pugin, Alain; Roblin, Gabriel

doi:10.1016/j.plaphy.2011.04.002

Cited by 22 publications

(14 citation statements)

References 77 publications

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“…Kinetics of changes in extracellular pH vary among elicitors, and do not predict their type or nature. Tobacco cells reacted to GME with an alkalinization response in a manner resembling the perception of several elicitors such as cyclodextrins, methyljasmonate, oligogalacturonides or cryptogein (Almagro et al, 2012;Binet et al, 1998), whereas the reaction with laminarin or ergosterol was transient, with a return to the resting value (Klarzynski et al, 2000;Vatsa et al, 2011). GME is a polyphenol-enriched extract without measurable jasmonic acid (Goupil et al, 2012) or methyljasmonate (data not shown).…”

Section: Discussionmentioning

confidence: 85%

Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells

Benouaret

Goujon

Goupil

2014

Plant Physiology and Biochemistry

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To cite this version:Razik Benouaret, Eric Goujon, Pascale Goupil. Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells. Plant Physiology and Biochemistry, Elsevier, 2014Elsevier, , 77, pp.84-89. <10.1016Elsevier, /j.plaphy.2014.01.021>. Author manuscript, published in Plant Physiology and Biochemistry (2014) 77: 84-89 DOI:10.1016/j.plaphy.2014 1 -A polyphenol-rich extract from grapes induced chemoperception in tobacco suspension cells -GME induced modification of plasma membrane properties -GME elicits defence genes in tobacco cells -GME-induced cell death was mediated by proteases and de novo protein synthesis -This study evidences the ability of GME to induce hypersensitive response Graphical abstractHighlights Abstract: Grape marc extract (GME) showed elicitor activity on suspension-cultured cells of tobacco. The BY-2 cells reacted to GME (0.25% and 0.125%) with a long-sustained pH rise in their growth medium. Using EGTA or LaCl 3 , we showed that extracellular alkalinization depended on Ca 2+ mobilization. The tobacco BY-2 cells challenged with GME promoted cell death and the upregulation of defence-related genes such as PR3, PAL and CCoAOMT. Cell death rate was quantified using an experimental calibrated Evans Blue assay. The GMEinduced cell death was dose-dependent and occurred in 24 h. Longer exposure increased the extent of tobacco cell death. To investigate a potential hypersensitive reaction, we tested the effect of various inhibitors of protein synthesis (cycloheximide) and proteases (aprotinin, pepstatin and E-64) on GME-induced cell death. All these chemicals reduced GME-induced cell death rate in 30 min. Overall, our findings indicate that GME elicits early perception events, defence reactions and cell death requiring protein synthesis and proteases.

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Section: Discussionmentioning

confidence: 85%

Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells

Benouaret

Goujon

Goupil

2014

Plant Physiology and Biochemistry

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“…Ergosterol acts as a MAMP molecule in tobacco and tomato plants, triggering a defence response characterised by the elicitation of the oxidative burst, the production of ROS [31], [32], alkalinisation of the external milieu and mobilisation of cytosolic calcium [30], [31], [125], [126]. Furthermore, ergosterol induces expression of some genes encoding pathogenesis-related proteins (PR1, PR3, and PR5) and enzymes participating in the defence response such as phenylalanine-ammonia lyase and sesquiterpene cyclase [32].…”

Section: Resultsmentioning

confidence: 99%

Multi-Platform Metabolomic Analyses of Ergosterol-Induced Dynamic Changes in Nicotiana tabacum Cells

et al. 2014

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Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used to investigate the effect of the fungal sterol, ergosterol, on the metabolome of cultured tobacco cells. Cell suspensions were treated with different concentrations (0–1000 nM) of ergosterol and incubated for different time periods (0–24 h). Intracellular metabolites were extracted with two methods: a selective dispersive liquid-liquid micro-extraction and a general methanol extraction. Chromatographic techniques (GC-FID, GC-MS, GC×GC-TOF-MS, UHPLC-MS) and 1H NMR spectroscopy were used for quantitative and qualitative analyses. Multivariate data analyses (PCA and OPLS-DA models) were used to extract interpretable information from the multidimensional data generated from the analytical techniques. The results showed that ergosterol triggered differential changes in the metabolome of the cells, leading to variation in the biosynthesis of secondary metabolites. PCA scores plots revealed dose- and time-dependent metabolic variations, with optimal treatment conditions being found to be 300 nM ergosterol and an 18 h incubation period. The observed ergosterol-induced metabolic changes were correlated with changes in defence-related metabolites. The ‘defensome’ involved increases in terpenoid metabolites with five antimicrobial compounds (the bicyclic sesquiterpenoid phytoalexins: phytuberin, solavetivone, capsidiol, lubimin and rishitin) and other metabolites (abscisic acid and phytosterols) putatively identified. In addition, various phenylpropanoid precursors, cinnamic acid derivatives and - conjugates, coumarins and lignin monomers were annotated. These annotated metabolites revealed a dynamic reprogramming of metabolic networks that are functionally correlated, with a high complexity in their regulation.

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“…Bacteria Harpin (HrpZ) Various plants [28,246,247] Flagellin Most plants except rice (e.g., Arabidopsis) [28,183] Cold shock proteins Solanaceae [28] Elongation factor (EF-Tu) Brassicaceae [28] Lipopolysaccharides (LPS) Arabidopsis, pepper, and tomato [28] Peptidoglycan Arabidopsis and tobacco [28] Oligogalacturonides Arabidopsis and tomato [108] Lipopeptides Tomato [248] Dimethylsulfide Maize and tobacco [249] Pseudobactin Several plants [214] Type-III secreted effector (T3E) Several plants [153] Tri-N-alkylated benzylamine derivative (NABD) Bean [219] 2,4-diacetylphloroglucinol (DAPG) Tomato, Arabidopsis [211,219,220] Pyocyanine and pyochelin Tomato [221] Exopolysaccharides Tobacco [228] N-acyl-L-homoserine lactone Tomato [229] Fungi B-glucans Several plants (e.g., rice) [28,[250][251][252][253] Chitin/chitosan Arabidopsis, rice, tomato, and wheat [28,[120][121][122][123][124] Cerebrosides A, C Rice [28,147] Ergosterol Grapevine, tomato, and tobacco [28,254,255] Xylanase Tobacco and tomato [28] HR-inducing protein Rice [256][257]…”

Section: Origin Elicitor Molecule Effects Shown In Referencesmentioning

confidence: 99%

Elicitor and Receptor Molecules: Orchestrators of Plant Defense and Immunity

Malik

Kumar

Nadarajah

2020

IJMS

235

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Pathogen-associated molecular patterns (PAMPs), microbe-associated molecular patterns (MAMPs), herbivore-associated molecular patterns (HAMPs), and damage-associated molecular patterns (DAMPs) are molecules produced by microorganisms and insects in the event of infection, microbial priming, and insect predation. These molecules are then recognized by receptor molecules on or within the plant, which activates the defense signaling pathways, resulting in plant’s ability to overcome pathogenic invasion, induce systemic resistance, and protect against insect predation and damage. These small molecular motifs are conserved in all organisms. Fungi, bacteria, and insects have their own specific molecular patterns that induce defenses in plants. Most of the molecular patterns are either present as part of the pathogen’s structure or exudates (in bacteria and fungi), or insect saliva and honeydew. Since biotic stresses such as pathogens and insects can impair crop yield and production, understanding the interaction between these organisms and the host via the elicitor–receptor interaction is essential to equip us with the knowledge necessary to design durable resistance in plants. In addition, it is also important to look into the role played by beneficial microbes and synthetic elicitors in activating plants’ defense and protection against disease and predation. This review addresses receptors, elicitors, and the receptor–elicitor interactions where these components in fungi, bacteria, and insects will be elaborated, giving special emphasis to the molecules, responses, and mechanisms at play, variations between organisms where applicable, and applications and prospects.

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Cytosolic calcium rises and related events in ergosterol-treated Nicotiana cells

Cited by 22 publications

References 77 publications

Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells

Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells

Multi-Platform Metabolomic Analyses of Ergosterol-Induced Dynamic Changes in Nicotiana tabacum Cells

Elicitor and Receptor Molecules: Orchestrators of Plant Defense and Immunity

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