Cytosolic and intranuclear calcium signals in rat basophilic leukemia cells as revealed by a confocal fluorescence Microscope

Nakato, Kazuhiro; Furuno, Tadahide; Inagaki, Kenji; Teshima, Reiko; Terao, Tadao; Nakanishi, Mamoru

doi:10.1111/j.1432-1033.1992.tb17343.x

Cited by 35 publications

(30 citation statements)

References 26 publications

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“…Furthermore, Ca 2ϩ is necessary for cell division, homeostasis, and survival (22)(23)(24) and regulates protein trafficking through the nuclear membrane (25). Ca 2ϩ is also essential for formation of cell processes; it regulates axon extension and pathfinding, controls formation of pseudopodia, and affects migration (26)(27)(28)(29). Glutamate receptor͞ion channel complexes, including the embryonic ones, are permeable to Ca 2ϩ (30)(31)(32).…”

Section: Discussionmentioning

confidence: 99%

Glutamate antagonists limit tumor growth

Rzeski

Turski

Ikonomidou

2001

Proc. Natl. Acad. Sci. U.S.A.

245

163

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Neuronal progenitors and tumor cells possess propensity to proliferate and to migrate. Glutamate regulates proliferation and migration of neurons during development, but it is not known whether it influences proliferation and migration of tumor cells. We demonstrate that glutamate antagonists inhibit proliferation of human tumor cells. Colon adenocarcinoma, astrocytoma, and breast and lung carcinoma cells were most sensitive to the antiproliferative effect of the N-methyl-d-aspartate antagonist dizocilpine, whereas breast and lung carcinoma, colon adenocarcinoma, and neuroblastoma cells responded most favorably to the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionate antagonist GYKI52466. The antiproliferative effect of glutamate antagonists was Ca2+ dependent and resulted from decreased cell division and increased cell death. Morphological alterations induced by glutamate antagonists in tumor cells consisted of reduced membrane ruffling and pseudopodial protrusions. Furthermore, glutamate antagonists decreased motility and invasive growth of tumor cells. These findings suggest anticancer potential of glutamate antagonists

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Section: Discussionmentioning

confidence: 99%

Glutamate antagonists limit tumor growth

Rzeski

Turski

Ikonomidou

2001

Proc. Natl. Acad. Sci. U.S.A.

245

163

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“…The brighter part of Fluo-3 fluorescence intensity was identical to the nucleus, suggesting that the FceRI-mediated Ca 2ϩ signals were transferred not only to the cytoplasm but also to the nucleus. 26) Based on ratio-imaging using Indo-1 (a ratiometric Ca 2ϩ -specific fluorescent probe), it was estimated that the Ca 2ϩ concentration was 200-300 nM higher in the nucleus than in the cytoplasm. The receptor-mediated nuclear Ca 2ϩ signals were also found in B lymphocytes and T lymphocytes.…”

Section: Calcium Signalsmentioning

confidence: 99%

Live Cell Imaging to Study Signaling Molecules in Allergic Reactions

Furuno

Nakanishi

2005

Biological & Pharmaceutical Bulletin

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Mast cells are widely distributed throughout the body, predominantly near blood vessels and nerves, and express effector functions in allergic reactions, inflammatory diseases, and host defense. The activation of mast cells results in secretion of the preformed chemical mediators in their granules by a regulated process of exocytosis and leads to synthesis and secretion of lipid mediators and cytokines. Their soluble factors contribute to allergic inflammation. Mast cells are associated with hypersensitivity reactions, not only in the classical immunoglobulin E (IgE)-dependent mechanism but also in an IgE-independent manner. In particular, investigations of potential anatomical and functional interactions between mast cells and the nervous system have recently attracted great interest. To understand these molecular mechanisms in mast cell activation, the ability to visualize, track, and quantify molecules and events in living mast cells is an essential and powerful tool. Recent dramatic advances in imaging technology and labeling techniques have enabled us to carry out these tasks with high spatiotemporal resolution using confocal laser scanning microscopes, green fluorescent protein and its derivatives, and image analysis systems. Here we review our investigations of the dynamic processes of intracellular signaling molecules, cellular structure, and interactions with neurons in mast cells to provide basic and valuable information for allergy and clinical immunology using these new imaging methods.

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“…Cells were observed with a CLSM (Zeiss, LSM-410; argon ion laser at 488 nm) and images were captured and analyzed using IBM compatible computer software. 6,[10][11][12] Differential interference images of the co-cultured cells were also measured by a Zeiss confocal laser scanning microscope (LSM-410).6) Neurite activation Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan and National Institute of Health Science, b Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. Received August 25, 2000; accepted November 9, 2000 Communication between nerves and mast cells is a prototypic demonstration of neuro-immune interaction.…”

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confidence: 99%

Bi-directional Relationship of in Vitro Mast Cell-Nerve Communication Observed by Confocal Laser Scanning Microscopy.

Suzuki

Furuno

Teshima³

et al. 2001

Biological & Pharmaceutical Bulletin

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During the last decade there has been an exponential increase in data illustrating that the immune and nervous systems are not disparate entities.1,2) The mast cell-nerve relationship has served as a prototypic association and has provided substantial evidence for bi-directional communication between nerves and immune cells.3) Early studies elegantly described the non-random spatial association of mast cells and nerves in a variety of tissues in which actual membranemembrane contacts could be observed. 4,5) To understand these events, we have recently studied direct neurite-mast cell communication using an in vitro co-culture approach and calcium imaging by confocal laser scanning microscopy (CLSM). Our results showed clearly that nervemast cell cross-talk can occur in the absence of an intermediary transducing cell and that the neuropeptide substance P, operating via NK-1 receptors, is an important mediator of this communication.6) In addition, we studied direct nervemast cell communication by atomic force microscopy (AFM). AFM showed precisely cell surface structures in which neurites attached to a pseudopodium and a cell body of a mast cell. 7) Our findings have implications for the neuroimmune signaling cascades that are likely to occur during airway inflammation, intestinal hypersensitivity, and other conditions in which mast cells feature. 6,7) However, the mast cell-nerve interaction is not a one-sided relationship but a bi-directional one.3) The consequent effects of such potentially reverberating circuits on local physiology may have great significance in the initiation or perpetuation of disease states, such as bronchial hyperreactivity and asthma, idiopathic functional bowel disorders, food allergy, and eczema.In the present work, we have studied in more detail the communication from mast cells to neurites. Our results clearly show that communication from mast cells to neurites exists in the in vitro nerve-mast cell co-culture system. MATERIALS AND METHODSNerve-Mast Cell Co-culture Following a published protocol, superior cervical ganglia (SCG) were dissected from newborn (0-48 h old) CBA mice (Japan SLC, Shizuoka, Japan) and rinsed in Hanks' balanced salt solution containing 10 mM HEPES (pH 7.4). Each ganglion was divided into two to four pieces and incubated for 60 min at 37°C in 2 ml of HEPES containing 0.125% trypsin (grade II; Sigma, St. Louis, MO, U.S.A.). The resultant cell suspension was plated at a density of 0.5-1ϫ10 4 nerve cells onto matrigel (Becton Dickinson, Bedford, MA, U.S.A.)-coated 35-mm diameter glass dishes. The neurons were grown in F12 culture medium (Life Technologies, Rockville, MD, U.S.A.) supplemented with 0.2 mM L-glutamine, 0.3% glucose, 3% antibiotic/antimycotic (A-7292) (all from Sigma), 10% FBS (BioWhittaker, Walkersville, MD, U.S.A.), and 50 ng/ml murine nerve growth factor (NGF, 2.5 S; Upstate Biotechnology, Lake placid, NY, U.S.A.). Nonganglionic cells were killed by an initial exposure to cytosine-b -D-arabinofuranoside (Ara-C, 10 Ϫ6 M; Sigma) for 24 h. Further deta...

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Cytosolic and intranuclear calcium signals in rat basophilic leukemia cells as revealed by a confocal fluorescence Microscope

Cited by 35 publications

References 26 publications

Glutamate antagonists limit tumor growth

Glutamate antagonists limit tumor growth

Live Cell Imaging to Study Signaling Molecules in Allergic Reactions

Bi-directional Relationship of in Vitro Mast Cell-Nerve Communication Observed by Confocal Laser Scanning Microscopy.

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