Cytoskeletal protein synthesis and organization in cultured mouse osteoblastic cells Effects of cell density

Lomri, Abderrahim; Marie, Pierre J.; Escurat, Michel; Portier, Marie‐Madeleine

doi:10.1016/0014-5793(87)80392-7

Cited by 11 publications

(4 citation statements)

References 26 publications

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“…33 In addition, during osteoblast differentiation, hMSCs morphologically change from fibroblast-like cells to cuboidal morphology, which is associated with changes in the cytoskeleton. 35 Similarly, during differentiation of hMSCs to adipocytes, significant changes in cell morphology takes place. 36, 37 Our data are in concordance with previous work showing that TGF β 1 treatment suppresses MSC proliferation and participates in cytoskeleton changes and thus hMSC differentiation.…”

Section: Discussionmentioning

confidence: 99%

Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization

et al. 2016

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Regenerative medicine is a novel approach for treating conditions in which enhanced bone regeneration is required. We identified transgelin (TAGLN), a transforming growth factor beta (TGFβ)-inducible gene, as an upregulated gene during in vitro osteoblastic and adipocytic differentiation of human bone marrow-derived stromal (skeletal) stem cells (hMSC). siRNA-mediated gene silencing of TAGLN impaired lineage differentiation into osteoblasts and adipocytes but enhanced cell proliferation. Additional functional studies revealed that TAGLN deficiency impaired hMSC cell motility and in vitro transwell cell migration. On the other hand, TAGLN overexpression reduced hMSC cell proliferation, but enhanced cell migration, osteoblastic and adipocytic differentiation, and in vivo bone formation. In addition, deficiency or overexpression of TAGLN in hMSC was associated with significant changes in cellular and nuclear morphology and cytoplasmic organelle composition as demonstrated by high content imaging and transmission electron microscopy that revealed pronounced alterations in the distribution of the actin filament and changes in cytoskeletal organization. Molecular signature of TAGLN-deficient hMSC showed that several genes and genetic pathways associated with cell differentiation, including regulation of actin cytoskeleton and focal adhesion pathways, were downregulated. Our data demonstrate that TAGLN has a role in generating committed progenitor cells from undifferentiated hMSC by regulating cytoskeleton organization. Targeting TAGLN is a plausible approach to enrich for committed hMSC cells needed for regenerative medicine application.

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Section: Discussionmentioning

confidence: 99%

Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization

et al. 2016

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“…First, actin and microtubules are abundant in osteoblasts (Aubin et al, 1983;Arena et al, 1991). During OB differentiation, cell morphology is found to alter from fibroblast-like to cuboidal morphology; this is accompanied by changes in actin cytoskeleton (Lomri et al, 1987). Second, bone is a tissue that is sensitive to mechanical stimulation.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 98%

Inhibiting actin depolymerization enhances osteoblast differentiation and bone formation in human stromal stem cells

et al. 2015

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Remodeling of the actin cytoskeleton through actin dynamics is involved in a number of biological processes, but its role in human stromal (skeletal) stem cells (hMSCs) differentiation is poorly understood. In the present study, we demonstrated that stabilizing actin filaments by inhibiting gene expression of the two main actin depolymerizing factors (ADFs): Cofilin 1 (CFL1) and Destrin (DSTN) in hMSCs, enhanced cell viability and differentiation into osteoblastic cells (OB) in vitro, as well as heterotopic bone formation in vivo. Similarly, treating hMSC with Phalloidin, which is known to stabilize polymerized actin filaments, increased hMSCs viability and OB differentiation. Conversely, Cytocholasin D, an inhibitor of actin polymerization, reduced cell viability and inhibited OB differentiation of hMSC. At a molecular level, preventing Cofilin phosphorylation through inhibition of LIM domain kinase 1 (LIMK1) decreased cell viability and impaired OB differentiation of hMSCs. Moreover, depolymerizing actin reduced FAK, p38 and JNK activation during OB differentiation of hMSCs, while polymerizing actin enhanced these signaling pathways. Our results demonstrate that the actin dynamic reassembly and Cofilin phosphorylation loop is involved in the control of hMSC proliferation and osteoblasts differentiation.

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“…The elastic properties of fibroblasts are regulated by the cytoskeleton, which provides the cell with rigidity, shape, and integrity. The cytoskeleton consists of microtubules, actin filaments, and intermediate filaments, and these components have different functional and mechanical properties [27] . Enhanced actin polymerization can modulate the stiffness of cells, whereas dense, highly cross-linked F-actin networks increase cellular stiffness, resulting in cells that are less susceptible to deformation [28] .…”

Section: Discussionmentioning

confidence: 99%

Effects of red ginseng on the elastic properties of human skin

Park

Han

Moon

et al. 2020

Journal of Ginseng Research

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Background Red ginseng contains components, including microelements, vitamins, essential oils, and fatty acids, that can be used in skincare to delay the aging process. We investigated the effects of red ginseng treatment on skin elasticity by assessing cellular stiffness and measuring collagen protein synthesis. Methods Human dermal fibroblasts were treated with red ginseng, and the resulting changes in stiffness were investigated using atomic force microscopy. Cytoskeletal changes and mRNA expression of biomarkers of aging, including that of procollagens I and VII, elastin, and fibrillin-1, were investigated. Collagen in a human skin equivalent treated with red ginseng was visualized via hematoxylin and eosin staining, scanning electron microscopy, and atomic force microscopy. Results and conclusion The stiffness of fibroblasts was significantly reduced by treatment with red ginseng concentrations of ≥ 0.8 mg/mL. The ratio of F-actin to G-actin decreased after treatment, which corresponded to a change in fibroblast stiffness. The storage modulus ( G ′) and loss modulus ( G ″) of the skin equivalent were both lowered by red ginseng treatment. This result indicates that the viscoelasticity of the skin equivalent can be restored by red ginseng treatment.

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Cytoskeletal protein synthesis and organization in cultured mouse osteoblastic cells Effects of cell density

Cited by 11 publications

References 26 publications

Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization

Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization

Inhibiting actin depolymerization enhances osteoblast differentiation and bone formation in human stromal stem cells

Effects of red ginseng on the elastic properties of human skin

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