Cytoskeletal organization following cannabinoid treatment in undifferentiated and differentiated PC12 cells

Tahir, Stephen K.; Trogadis, Judy; Stevens, J. R.; Zimmerman, Arthur M.

doi:10.1139/o92-162

Cited by 58 publications

(55 citation statements)

References 25 publications

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“…This may be linked to structural changes or remodeling occurring after D 9 -THC exposure in adolescents and is consistent with observations of cytoarchitectural changes occurring with cannabinoid treatment (Tagliaferro et al, 2006) and other reports of altered expression of the structural-related proteins tubulin and actin (Tahir et al, 1992;Wilson et al, 1996). MBP is a major constituent of the myelin sheath that allows fast saltatory conduction of nerve pulses (Vanrobaeys et al, 2005).…”

Section: Protein Expression Profiles In Adolescent Brainssupporting

confidence: 88%

Adolescent Rats Find Repeated Δ9-THC Less Aversive Than Adult Rats but Display Greater Residual Cognitive Deficits and Changes in Hippocampal Protein Expression Following Exposure

Quinn

Matsumoto

Callaghan

et al. 2007

Neuropsychopharmacol

272

226

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The current study examined whether adolescent rats are more vulnerable than adult rats to the lasting adverse effects of cannabinoid exposure on brain and behavior. Male Wistar rats were repeatedly exposed to D-9-tetrahydrocannabinol (D 9 -THC, 5 mg/kg i.p.) in a place-conditioning paradigm during either the adolescent (post-natal day 28 + ) or adult (post-natal day 60 + ) developmental stages. Adult rats avoided a D 9 -THC-paired environment after either four or eight pairings and this avoidance persisted for at least 16 days following the final D 9 -THC injection. In contrast, adolescent rats showed no significant place aversion. Adult D 9 -THC-treated rats produced more vocalizations than adolescent rats when handled during the intoxicated state, also suggesting greater drug-induced aversion. After a 10-15 day washout, both adult and adolescent D 9 -THC pretreated rats showed decreased social interaction, while only D 9 -THC pretreated adolescent rats showed significantly impaired object recognition memory. Seventeen days following their last D 9 -THC injection, rats were euthanased and hippocampal tissue processed using two-dimensional gel electrophoresis proteomics. There was no evidence of residual D 9 -THC being present in blood at this time. Proteomic analysis uncovered 27 proteins, many involved in regulating oxidative stress/mitochondrial functioning and cytoarchitecture, which were differentially expressed in adolescent D 9 -THC pretreated rats relative to adolescent controls. In adults, only 10 hippocampal proteins were differentially expressed in D 9 -THC compared to vehicle-pretreated controls. Overall these findings suggest that adolescent rats find repeated D 9 -THC exposure less aversive than adults, but that cannabinoid exposure causes greater lasting memory deficits and hippocampal alterations in adolescent than adult rats.

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Section: Protein Expression Profiles In Adolescent Brainssupporting

confidence: 88%

Adolescent Rats Find Repeated Δ9-THC Less Aversive Than Adult Rats but Display Greater Residual Cognitive Deficits and Changes in Hippocampal Protein Expression Following Exposure

Quinn

Matsumoto

Callaghan

et al. 2007

Neuropsychopharmacol

272

226

View full text Add to dashboard Cite

show abstract

“…These cells have been well characterized and have become a model for many studies related to identification and characterization of neuronal signaling pathways (Heneka et al, 1998), to study cell death (Dubreuil et al, 2003;Kitazawa et al, 2004;Ulloth et al, 2003;Yang et al, 2004), especially apoptosis, in neuronal cells and cytoskeletal structure and function (Kobayashi and Mundel, 1998;Nakayama et al, 2001;Okabe and Hirokawa, 1988;Tahir et al, 1992). Although no other types of cells were examined in the present study, cell viability studies and onset rate dependency of traumatic injury produced on cells with CCSD were similar to previous studies conducted with human derived neuronal cells (Ellis et al, 1995;LaPlaca and Thibault, 1998).…”

Section: Discussionsupporting

confidence: 67%

The Effect of Poloxamer-188 on Neuronal Cell Recovery from Mechanical Injury

Serbest

Horwitz

Barbee

2005

Journal of Neurotrauma

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Neuronal injury resulting from mechanical deformation is poorly characterized at the cellular level. The immediate structural consequences of the mechanical loading lead to a variety of inter-and intra-cellular signaling events that interact on multiple time and length scales. Thus, it is often difficult to establish cause-and-effect relationships such that appropriate treatment strategies can be devised. In this report, we showed that treating mechanically injured neuronal cells with an agent that promotes the resealing of disrupted plasma membranes rescues them from death at 24 h post-injury. A new in vitro model was developed to allow uniform mechanical loading conditions with precisely controlled magnitude and onset rate of loading. Injury severity increased monotonically with increasing peak shear stress and was strongly dependent on the rate of loading as assessed with the MTT cell viability assay, 24 h post-injury. Mechanical injury produced an immediate disruption of membrane integrity as indicated by a rapid and transient release of LDH. For the most severe injury, cell viability decreased approximately 40% with mechanical trauma compared to sham controls. Treatment of cells with Poloxamer 188 at 15 min post-injury restored long-term viability to control values. These data establish membrane integrity as a novel therapeutic target in the treatment of neuronal injury.

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“…A similar mechanism was observed in PC12 cells. 29,30 Depending on the status of differentiation the amount of microtubules and microfilaments in PC12 was altered.…”

Section: Cannabinoids Alter the Adhesiveness And Stiffness Of Tumor Cmentioning

confidence: 99%

The influence of biomechanical properties and cannabinoids on tumor invasion

Hohmann

Grabiec

Ghadban

et al. 2016

Cell Adhesion & Migration

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Background: Cannabinoids are known to have an anti-tumorous effect, but the underlying mechanisms are only sparsely understood. Mechanical characteristics of tumor cells represent a promising marker to distinguish between tumor cells and the healthy tissue. We tested the hypothesis whether cannabinoids influence the tumor cell specific mechanical and migratory properties and if these factors are a prognostic marker for the invasiveness of tumor cells.Methods: 3 different glioblastoma cell lines were treated with cannabinoids and changes of mechanical and migratory properties of single cells were measured using atomic force microscopy and time lapse imaging. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures.Results: We found that cannabinoids are capable of influencing migratory and mechanical properties in a cell line specific manner. A network analysis revealed a correlation between a "generalized stiffness" and the invasiveness for all tumor cell lines after 3 and 4 d of invasion time: Conclusions: Here we could show that a "generalized stiffness" is a profound marker for the invasiveness of a tumor cell population in our model and thus might be of high clinical relevance for drug testing. Additionally cannabinoids were shown to be of potential use for therapeutic approaches of glioblastoma.

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Cytoskeletal organization following cannabinoid treatment in undifferentiated and differentiated PC12 cells

Cited by 58 publications

References 25 publications

Adolescent Rats Find Repeated Δ9-THC Less Aversive Than Adult Rats but Display Greater Residual Cognitive Deficits and Changes in Hippocampal Protein Expression Following Exposure

Adolescent Rats Find Repeated Δ9-THC Less Aversive Than Adult Rats but Display Greater Residual Cognitive Deficits and Changes in Hippocampal Protein Expression Following Exposure

The Effect of Poloxamer-188 on Neuronal Cell Recovery from Mechanical Injury

The influence of biomechanical properties and cannabinoids on tumor invasion

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