Cytosine chemoreceptor McpC in Pseudomonas putida F1 also detects nicotinic acid

Parales, Rebecca E.; Nesteryuk, Vasyl; Hughes, Jonathan G.; Luu, Rita A.; Ditty, Jayna L.

doi:10.1099/mic.0.081968-0

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…McpC (orthologous to PP0584) ( Fig. 3) was identified as a chemoreceptor for cytosine (172) and nicotinic acid (173). Another study led to the identification of a chemoreceptor, termed PcaY, which is an ortholog of PP2643 (Fig.…”

Section: Pseudomonasmentioning

confidence: 99%

Sensory Repertoire of Bacterial Chemoreceptors

Ortega

Zhulin

Krell

2017

Microbiol Mol Biol Rev

184

223

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Chemoreceptors in bacteria detect a variety of signals and feed this information into chemosensory pathways that represent a major mode of signal transduction. The five chemoreceptors from have served as traditional models in the study of this protein family. Genome analyses revealed that many bacteria contain much larger numbers of chemoreceptors with broader sensory capabilities. Chemoreceptors differ in topology, sensing mode, cellular location, and, above all, the type of ligand binding domain (LBD). Here, we highlight LBD diversity using well-established and emerging model organisms as well as genomic surveys. Nearly a hundred different types of protein domains that are found in chemoreceptor sequences are known or predicted LBDs, but only a few of them are ubiquitous. LBDs of the same class recognize different ligands, and conversely, the same ligand can be recognized by structurally different LBDs; however, recent studies began to reveal common characteristics in signal-LBD relationships. Although signals can stimulate chemoreceptors in a variety of different ways, diverse LBDs appear to employ a universal transmembrane signaling mechanism. Current and future studies aim to establish relationships between LBD types, the nature of signals that they recognize, and the mechanisms of signal recognition and transduction.

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Section: Pseudomonasmentioning

confidence: 99%

Sensory Repertoire of Bacterial Chemoreceptors

Ortega

Zhulin

Krell

2017

Microbiol Mol Biol Rev

184

223

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“…To identify the chemoreceptor for alcohols, mutant strains with various receptor gene deletions were screened for the ability to respond to ethanol. We have been working toward generating a "gutted" strain lacking all 27 of the putative MCP and MCP-like genes in P. putida 31), the aromatic acid receptor PcaY (16), and the nicotinic acid/cytosine receptor McpC (13,18). In addition, four genes encoding receptors of unknown function were also deleted in this strain (locus tags Pput_0342, Pput_3459, Pput_4234, and Pput_4764).…”

Section: Resultsmentioning

confidence: 99%

“…A conserved set of cytoplasmic chemotaxis proteins (CheA, CheY, CheW, CheR, and CheB), together with Ն20 membrane-spanning methylaccepting chemotaxis proteins (MCPs), mediate responses to a wide range of organic and inorganic attractants in pseudomonads (9,10). For example, known attractants for Pseudomonas putida strains include various amino acids, organic acids (11,12), purines and pyrimidines (13,14), polyamines (15), aromatic acids (16)(17)(18), and aromatic hydrocarbons (19)(20)(21). However, to our knowledge, chemotaxis to alcohols by Pseudomonas spp.…”

mentioning

confidence: 96%

Chemotaxis of Pseudomonas putida F1 to Alcohols Is Mediated by the Carboxylic Acid Receptor McfP

Zhang

Hughes

Subuyuj

et al. 2019

Appl Environ Microbiol

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Although alcohols are toxic to many microorganisms, they are good carbon and energy sources for some bacteria, including many pseudomonads. However, most studies that have examined chemosensory responses to alcohols have reported that alcohols are sensed as repellents, which is consistent with their toxic properties. In this study, we examined the chemotaxis of Pseudomonas putida strain F1 to n-alcohols with chain lengths of 1 to 12 carbons. P. putida F1 was attracted to all n-alcohols that served as growth substrates (C2 to C12) for the strain, and the responses were induced when cells were grown in the presence of alcohols. By assaying mutant strains lacking single or multiple methyl-accepting chemotaxis proteins, the receptor mediating the response to C2 to C12 alcohols was identified as McfP, the ortholog of the P. putida strain KT2440 receptor for C2 and C3 carboxylic acids. Besides being a requirement for the response to n-alcohols, McfP was required for the response of P. putida F1 to pyruvate, l-lactate, acetate, and propionate, which are detected by the KT2440 receptor, and the medium- and long-chain carboxylic acids hexanoic acid and dodecanoic acid. β-Galactosidase assays of P. putida F1 carrying an mcfP-lacZ transcriptional fusion showed that the mcfP gene is not induced in response to alcohols. Together, our results are consistent with the idea that the carboxylic acids generated from the oxidation of alcohols are the actual attractants sensed by McfP in P. putida F1, rather than the alcohols themselves. IMPORTANCE Alcohols, released as fermentation products and produced as intermediates in the catabolism of many organic compounds, including hydrocarbons and fatty acids, are common components of the microbial food web in soil and sediments. Although they serve as good carbon and energy sources for many soil bacteria, alcohols have primarily been reported to be repellents rather than attractants for motile bacteria. Little is known about how alcohols are sensed by microbes in the environment. We report here that catabolizable n-alcohols with linear chains of up to 12 carbons serve as attractants for the soil bacterium Pseudomonas putida, and rather than being detected directly, alcohols appear to be catabolized to acetate, which is then sensed by a specific cell-surface chemoreceptor protein.

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“…It was determined that cytidine was degraded by the enzymes nucleoside hydrolase, uridine phosphorylase and cytosine deaminase [118]. Another study found that the pyrimidine base cytosine served as a chemoattractant for P. putida, with cytosine deaminase degrading cytosine to uracil, which provides a nitrogen source to the cells [130,131].…”

Section: Pseudomonas Putida Homology Groupmentioning

confidence: 99%

Pyrimidine Biosynthesis and Ribonucleoside Metabolism in Species of Pseudomonas

West

2023

Fermentation

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Pyrimidine biosynthesis and ribonucleoside metabolism in species of Pseudomonas was the focus of this review, in relation to their current taxonomic assignments in different homology groups. It was of interest to learn whether pyrimidine biosynthesis in taxonomically related species of Pseudomonas was regulated in a similar fashion by pyrimidine base supplementation or by pyrimidine limitation of pyrimidine auxotrophic strains. It was concluded that the regulation of pyrimidine biosynthesis in Pseudomonas species could not be correlated with their taxonomic assignment into a specific homology group. Pyrimidine ribonucleoside metabolism in Pseudomonas species primarily involved the pyrimidine ribonucleoside salvage enzymes nucleoside hydrolase and cytosine deaminase, independently of the Pseudomonas homology group to which the species was assigned. Similarly, pyrimidine base catabolism was shown to be active in different taxonomic homology groups of Pseudomonas. Although the number of studies exploring the catabolism of the pyrimidine bases uracil and thymine was limited in scope, it did appear that the presence of the pyrimidine base reductive pathway of pyrimidine catabolism was a commonality observed for the species of Pseudomonas investigated. There also appeared to be a connection between pyrimidine ribonucleoside degradation and the catabolism of pyrimidine bases in providing a cellular source of carbon or nitrogen independently of which homology group the species of Pseudomonas were assigned to.

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Cytosine chemoreceptor McpC in Pseudomonas putida F1 also detects nicotinic acid

Cited by 8 publications

References 52 publications

Sensory Repertoire of Bacterial Chemoreceptors

Sensory Repertoire of Bacterial Chemoreceptors

Chemotaxis of Pseudomonas putida F1 to Alcohols Is Mediated by the Carboxylic Acid Receptor McfP

Pyrimidine Biosynthesis and Ribonucleoside Metabolism in Species of Pseudomonas

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