Cytoprotective potential of tiron and methyl palmitate against acetaminophen-induced acute liver injury

Abstract

Acute liver injury is a debilitating disorder associated with loss of synthetic and detoxifying functions of the liver. This investigation was designed to assess cytoprotective efficacy of daily oral tiron (300 mg/kg) and daily oral methyl palmitate (300 mg/kg) against acetaminophen-induced acute liver injury. Rats were orally pretreated with either tiron or methyl palmitate at doses (300 mg/kg) for 7 days prior to oral acetaminophen (3 g/kg). Biochemical assay of markers of hepatotoxicity indices and oxidative stress was undertaken. Expression of inflammatory cytokine IL-6 was also evaluated. Histopathological examination of liver specimens was carried out as well. Both methyl palmitate and tiron significantly reversed the acetaminophen-induced elevation of biochemical markers (ALT, AST, and ALP) with restoration of SOD levels. Serum albumin levels and GSH liver contents increased, but in a nonsignificant manner. Moreover, methyl palmitate and tiron significantly decreased the level of serum LDH and serum IL-6 levels. Histopathology revealed that tiron markedly reduced the extent of acetaminophen-induced necrosis and methyl palmitate moderately decreased the necrosis in liver tissue. Methyl palmitate (300 mg/kg) and tiron (300 mg/kg) demonstrated promising hepatoprotective effects against acetaminophen-induced acute liver injury via modulation of inflammatory response and alleviation of the oxidative stress, allowing the preservation of hepatic functions.

Fresh Seed Supplementation of Syzygium Cumini Attenuated Oxidative Stress, Inflammation, Fibrosis, Iron Overload, Hepatic Dysfunction and Renal Injury in Acetaminophen Induced Rats

“…Literatures also explored that once there is any necrosis or injury observed inside the organ, local fibroblast tissues are activated and secrete collagen as well as extracellular matrix [13], our study revealed that antioxidant property of Syzygium cumini seed significantly reduced fibrosis and collagen deposition ( Figures 5D and 6D). Similar kind of attenuation was observed by Shoeib et al and Leite et al [58,59]. On the other hand, tissue iron is highly monitored and maintained by heme oxygenase (HO) which is often degraded by foreign harmful stimuli and initiate several types of dysfunctions like iron overload, hypertrophy and apoptosis ( Figures 7A-7D) [10,60].…”

Sulforaphane protects against sodium valproate–induced acute liver injury

“…Antioxidants have been repeatedly reported to offer significant protection against various forms of liver injury (Said et al 2012;Shoeib et al 2015;Stravitz and Kramer 2009). Endogenous antioxidant defenses are reported to be activated to protect against oxidative injury (Bir et al 2012).…”

Investigation of the Effects of Resveratrol on Paracetamol Toxicity Established in Hep3B Cells

“…It arises through the prevention of mitochondrial dysfunction and suppression of oxidative stress [42]. Similarly, scientific studies carried out in recent years have shown that IL-6 level increases in paracetamol-induced acute liver toxicity, and the therapeutic agents administered following this decrease the IL-6 level [43,44]. In the current study, it was shown that the paracetamol group caused an increase in the IL-6 level, but resveratrol administration showed efficacy even at different doses, as in TNF-alpha, and caused a decrease.…”