Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro

Bai, Hui; Li, Tingting; Yu, Yao; Zhou, Ningcong; Kou, Huijuan; Guo, Yingying; Yan, Peishi

doi:10.3390/cells10020258

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…Taurine alleviated the rates of apoptosis in bovine mammary epithelial cells and suppressed the expression of heat shock factor 1 (HSF1) mRNA and protein under heat-stress conditions, but not HSP90 [53]. Our findings align with the research conducted by Bai, Li, Yu, Zhou, Kou, Guo, Yang and Yan [53], as we also observed selective response of CopA3 towards HSPs. The compounds under investigation as heat-stress relievers have shown the capability to restore apoptosis and protein expression levels, which were increased by heat stress, back to normal conditions.…”

Section: Discussionsupporting

confidence: 90%

“…Taurine, which is recognized as a natural antioxidant found in various animal tissues [51], has also been investigated as a potential remedy for heat stress [52]. Taurine alleviated the rates of apoptosis in bovine mammary epithelial cells and suppressed the expression of heat shock factor 1 (HSF1) mRNA and protein under heat-stress conditions, but not HSP90 [53]. Our findings align with the research conducted by Bai, Li, Yu, Zhou, Kou, Guo, Yang and Yan [53], as we also observed selective response of CopA3 towards HSPs.…”

Section: Discussionmentioning

confidence: 99%

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Insect Peptide CopA3 Mitigates the Effects of Heat Stress on Porcine Muscle Satellite Cells

Lee,

Belal,

Lin

et al. 2023

Animals

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Heat stress inhibits cell proliferation as well as animal production. Here, we aimed to demonstrate that 9-mer disulfide dimer peptide (CopA3) supplementation stabilizes porcine muscle satellite cell (PMSC) proliferation and heat shock protein (HSP) expression at different temperatures. Therefore, we investigated the beneficial effects of CopA3 on PMSCs at three different temperatures (37, 39, and 41 °C). Based on temperature and CopA3 treatment, PMSCs were divided into six different groups including treatment and control groups for each temperature. Cell viability was highest with 10 µg/mL CopA3 and decreased as the concentration increased in a dose-dependent manner. CopA3 significantly increased the cell viability at all temperatures at 24 and 48 h. It significantly decreased apoptosis compared to that in the untreated groups. In addition, it decreased the apoptosis-related protein, Bcl-2-associated X (BAX), expression at 41 °C. Notably, temperature and CopA3 had no effects on the apoptosis-related protein, caspase 3. Expression levels of HSP40, HSP70, and HSP90 were significantly upregulated, whereas those of HSP47 and HSP60 were not affected by temperature changes. Except HSP90, CopA3 did not cause temperature-dependent changes in protein expression. Therefore, CopA3 promotes cell proliferation, inhibits apoptosis, and maintains stable HSP expression, thereby enhancing the heat-stress-tolerance capacity of PMSCs.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Insect Peptide CopA3 Mitigates the Effects of Heat Stress on Porcine Muscle Satellite Cells

Lee,

Belal,

Lin

et al. 2023

Animals

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“…In addition, the apoptosis rate of mammary epithelial cells in dairy cows was increased under HS conditions, and cytoskeletal and cell transport functions were disturbed (11)(12)(13). In modern dairy farms, managers adopt a variety of approaches to alleviate or prevent the occurrence of HS in dairy cows, and some feed additives [betaine (14), choline (15), taurine (16) and methionine (17)] seem to be effective in alleviating negative effects of HS.…”

Section: Introductionmentioning

confidence: 99%

Varying the ratio of Lys: Met through enhancing methionine supplementation improved milk secretion ability through regulating the mRNA expression in bovine mammary epithelial cells under heat stress

Fu,

You,

Zeng

et al. 2024

Front. Vet. Sci.

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IntroductionThe ratio of lysine (Lys) to methionine (Met) with 3.0: 1 is confirmed as the “ideal” profile for milk protein synthesis, but whether this ratio is suitable for milk protein synthesis under HS needs to be further studied.MethodsTo evaluate the molecular mechanism by which HS and Lys to Met ratios affect mammary cell functional capacity, an immortalized bovine mammary epithelial cell line (MAC-T) is incubated with 5 doses of Met while maintaining a constant concentration of Lys. The MAC-T cells was treated for 6 h as follow: Lys: Met 3.0: 1 (control 37°C and IPAA 42°C) or treatments under HS (42°C) with different ratios of Lys: Met at 2.0: 1 (LM20), 2.5: 1 (LM25), 3.5: 1 (LM35) and 4.0: 1 (LM40). RNA sequencing was used to assess transcriptome-wide alterations in mRNA abundance.ResultsThe significant difference between control and other groups was observed base on PCA analysis. A total of 2048 differentially expressed genes (DEGs) were identified in the IPAA group relative to the control group. Similarly, 226, 306, 148, 157 DEGs were detected in the LM20, LM25, LM35 and LM40 groups, respectively, relative to the IPAA group. The relative mRNA abundance of HSPA1A was upregulated and anti-apoptotic genes (BCL2L1 and BCL2) was down-regulated in the IPAA group, compared to the control group (p < 0.05). Compared with the IPAA group, the relative mRNA abundance of anti-apoptotic genes and casein genes (CSN1S2 and CSN2) was up-regulated in the LM25 group (p < 0.05). The DEGs between LM25 and IPAA groups were associated with the negative regulation of transcription RNA polymerase II promoter in response to stress (GO: 0051085, DEGs of BAG3, DNAJB1, HSPA1A) as well as the mTOR signaling pathway (ko04150, DEGs of ATP6V1C2, WNT11, WNT3A, and WNT9A). Several DEGs involved in amino acids metabolism (AFMID, HYKK, NOS3, RIMKLB) and glycolysis/gluconeogenesis (AFMID and MGAT5B) were up-regulated while DEGs involved in lipolysis and beta-oxidation catabolic processes (ALOX12 and ALOX12B) were down-regulated.ConclusionThese results suggested that increasing Met supply (Lys: Met at 2.5: 1) may help mammary gland cells resist HS-induced cell damage, while possibly maintaining lactation capacity through regulation of gene expression.

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“…Previous studies have shown that taurine supplementation promotes milk fat and protein synthesis and alleviates oxidative stress and inflammation in bovine mammary epithelial cells ( 20 , 21 ). Moreover, taurine can reverse the decreases in the activity of superoxide dismutase and glutathione peroxidase induced by HS, then alleviate cellular oxidative stress, and thus protect bovine mammary epithelial cells against HS ( 22 ). Furthermore, the protective effect of taurine on mammary glands has been achieved by attenuating mammary gland epithelial integrity damage and inflammatory response under HS conditions ( 23 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of taurine on metabolomics of bovine mammary epithelial cells under high temperature conditions

Liu,

Liang,

Luo

et al. 2024

Front. Vet. Sci.

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High temperature induces heat stress, adversely affecting the growth and lactation performance of cows. Research has shown the protective effect of taurine against hepatotoxicity both in vivo and in vitro. This study aimed to investigate the effect of taurine on the metabolomics of mammary epithelial cells of dairy cows under high-temperature conditions. Mammary epithelial cells were exposed to 0 mmol/L (HS, control), 8 mmol/L (HT-8), and 32 mmol/L (HT-32) of taurine, then incubated at 42°C for 6 h. Metabolomics analysis was conducted using Liquid Chromatograph Mass Spectrometer (LC–MS). Compared with the HS group, 2,873 and 3,243 metabolites were detected in the HT-8 group in positive and negative ion modes. Among these, 108 and 97 metabolites were significantly upregulated in positive and negative ion modes, while 60 and 166 metabolites were downregulated. Notably, 15 different metabolites such as palmitic acid, adenine and hypoxanthine were screened out in the HT-8 group. Compared with the HS group, 2,873 and 3,243 metabolites were, respectively, detected in the HT-32 group in the positive and negative ion modes. Among those metabolites, 206 metabolites were significantly up-regulated, while 206 metabolites were significantly downregulated in the positive mode. On the other hand, 497 metabolites were significantly upregulated in the negative mode, while 517 metabolites were reported to be downregulated. Noteworthy, 30 distinct metabolites, such as palmitic acid, phytosphingosine, hypoxanthine, nonanoic acid, and octanoic acid, were screened out in the HT-32 group. KEGG enrichment analysis showed that these metabolites were mainly involved in lipid metabolism, purine metabolism and other biological processes. Overall, our study indicates that taurine supplementation alters the metabolites primarily associated with purine metabolism, lipid metabolism and other pathways to alleviate heat stress in bovine mammary epithelial cells.

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Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro

Cited by 11 publications

References 44 publications

Insect Peptide CopA3 Mitigates the Effects of Heat Stress on Porcine Muscle Satellite Cells

Insect Peptide CopA3 Mitigates the Effects of Heat Stress on Porcine Muscle Satellite Cells

Varying the ratio of Lys: Met through enhancing methionine supplementation improved milk secretion ability through regulating the mRNA expression in bovine mammary epithelial cells under heat stress

Effects of taurine on metabolomics of bovine mammary epithelial cells under high temperature conditions

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