Cytoprotective effects of melatonin on zoledronic acid-treated human osteoblasts

Camacho-Alonso, Fabio; Urrutia-Rodríguez, I.; Oñate-Cabrerizo, Daniel; Sánchez, Ricardo Elías Oñate; Rodríguez‐Lozano, Francisco Javier

doi:10.1016/j.jcms.2017.04.006

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…In the present study, alkaline phosphatase activity was verified at day 10 and an increase in mineral nodules was observed for the concentration of 0.1mM MLT, in agreement with studies that suggest that the hormone may have a regulatory effect on bone growth 26,27 . Previous studies 13,14,28 demonstrated that melatonin was able to regulate osteoblastic function via the MT2 receptor, which showed greater expression in bone during the experiments. In addition, inactivation of this receptor, leaving only MT1 receptor active, negatively affected cell differentiation, proliferation and deposition of the mineralized matrix, thus reiterating the promising role of melatonin as a potential strategy to stimulate bone neoformation.…”

Section: Discussionmentioning

confidence: 85%

“…It has been demonstrated that the cytoprotective effects of MLT on osteoblasts are noted mainly at low concentrations and shorter times, using concentrations of 1, 10, 50, 100 and 200 mM for incubation periods of 24, 48 and 72h, showing the cytoprotective effect promoted by MLT in cells treated with bisfosfonate 26,27,28,29 . There is, however, great variability in MLT concentrations across the literature, ranging from 0.001 to 2.5mM in osteoblastic cells of the MC3T3 lineage 9,10,18 .…”

Section: Discussionmentioning

confidence: 98%

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Melatonin has a stimulatory effect on osteoblasts by upregulating COL-I and OPN expression/secretion.

Dalla-Costa¹,

Yurtsever²,

Penteado³

et al. 2020

Acta Odontol Latinoam

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Melatonin (MLT) is a potential signaling molecule in the homeostasis of bone metabolism and may be an important mediator of bone formation and stimulation. The aim of this in vitro study was to evaluate the effect of MLT on the viability, mRNA/protein expression and mineralization of pre-osteoblastic cells. The concentrations 5, 2.5, 1, 0.1 and 0.01 mM MLT were tested on pre-osteoblastic cells (MC3T3) compared to control (no MLT), evaluating proliferation and cell viability (C50), gene expression (RT-PCR) and secretion (ELISA) of COL-I and OPN at 24h, 48h and 72h, and the formation of mineral nodules (alizarin red and fast red) after 10 days of treatment. MLT at 5 and 2.5 mM proved to be cytotoxic (C50), so only 0.01, 0.1 and 1 mM were used for the subsequent analyses. OPN mRNA expression increased with MLT at 0.1 mM - 1 mM, which was followed by increased secretion of OPN both at 24h and 72h compared to the remaining groups (p <0.05). COL-I mRNA and COL-1 secretion followed the same pattern as OPN at 0.1 mM MLT at 72h of treatment (p <0.05). Regarding mineralization, all MLT doses (except 1mM) caused an increase (p <0.05) in the formation of mineral nodules compared to the control. Melatonin at 0.01mM - 1mM had a stimulatory effect on osteoblasts by upregulating COL-I and OPN expression/ secretion and mineralization, thereby fostering osteogenesis.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 98%

Melatonin has a stimulatory effect on osteoblasts by upregulating COL-I and OPN expression/secretion.

Dalla-Costa¹,

Yurtsever²,

Penteado³

et al. 2020

Acta Odontol Latinoam

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“…One study analyzed the gene expression of RANKL and osteoprotegerin, 109 and four trials concentrated on the positive effects of low-level laser therapy and herbal extracts to counteract MRONJ development. 107 , 113 – 115 Thirteen studies analyzed only one BP; of these, 10 studies used zoledronate (0.01–100 μM), 1 used pamidronate (10 −5 , 3 × 10 −5 , 6 × 10 −5 , and 10 −4 M/L), 1 used clodronate (10 −5 to 10 −9 mM/L), and 1 used alendronate (0.01–100 μM). 113 , 114 , 116 – 118 The remaining 11 studies applied a combination of zoledronate, ibandronate, pamidronate, clodronate, and alendronate at concentrations varying from 0.00001 to 500 μM.…”

Section: Resultsmentioning

confidence: 99%

“… 113 , 114 , 116 – 118 The remaining 11 studies applied a combination of zoledronate, ibandronate, pamidronate, clodronate, and alendronate at concentrations varying from 0.00001 to 500 μM. 115 , 119 – 121 Table 2 summarizes all studies that evaluated osteoblasts in MRONJ.…”

Section: Resultsmentioning

confidence: 99%

Pathogenesis of medication-related osteonecrosis of the jaw: a comparative study of in vivo and in vitro trials

et al. 2018

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ObjectiveThis study was performed to determine whether the results of prevailing in vivo and in vitro studies offer a reliable model for investigation of medication-related osteonecrosis of the jaw (MRONJ).MethodsEmbase, Medline, and the Cochrane Library were searched for articles published from September 2003 to June 2017 involving experimental approaches to the pathogenesis of MRONJ. In vivo and in vitro trials were analyzed with respect to the scientific question, study design, methodology, and results.ResultsOf 139 studies, 87, 46, and 6 conducted in vivo, in vitro, and both in vivo and in vitro experiments, respectively. Rats, mice, dogs, minipigs, sheep, and rabbits were the preferred animal models used. Osteoblasts, osteoclasts, fibroblasts, keratinocytes, macrophages, and human umbilical vein endothelial cells were the preferred cell types. Zoledronate, alendronate, ibandronate, and risedronate were the most frequent bisphosphonates used. MRONJ was most reliably induced in minipigs because of the close relationship with human bone physiology. In vitro studies showed that reduced viability, growth, and migration of cells in the bone and soft tissues were causative for MRONJ. Other than exposed jawbone after tooth extraction, no reliable cofactors were found.ConclusionThe minipig is the most suitable animal model for MRONJ.

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The effects of melatonin on the viability and osteogenic/odontogenic differentiation of human stem cells from the apical papilla

Karkehabadi,

Abbasi,

Najafi

et al. 2023

Mol Biol Rep

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Cytoprotective effects of melatonin on zoledronic acid-treated human osteoblasts

Cited by 6 publications

References 36 publications

Melatonin has a stimulatory effect on osteoblasts by upregulating COL-I and OPN expression/secretion.

Melatonin has a stimulatory effect on osteoblasts by upregulating COL-I and OPN expression/secretion.

Pathogenesis of medication-related osteonecrosis of the jaw: a comparative study of in vivo and in vitro trials

The effects of melatonin on the viability and osteogenic/odontogenic differentiation of human stem cells from the apical papilla

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