Cytoprotective effects of Bergenia ciliata Sternb, extract on gastric ulcer in rats

Guerra

et al. 2018

IJMS

Kalanchoe brasiliensis and Kalanchoe pinnata are used interchangeably in traditional medicine for treating peptic ulcers and inflammatory problems. In this context, this study aims to characterize the chemical constituents and evaluate the gastroprotective activity of the leaf juices of the two species in acute gastric lesions models. Thin Layer Chromatography (TLC) and Ultra High Performance Liquid Chromatography coupled to Mass Spectrometer (UHPLC-MS) were performed for chemical characterization. Wistar rats were pre-treated orally with leaf juices (125, 250 and 500 mg/kg) or ranitidine (50 mg/kg). The peaks observed in the chromatogram of K. brasiliensis showed similar mass spectra to flavonoid glycosides derived from patuletin and eupafolin, while K. pinnata showed mass spectra similar to compounds derived from quercetin, patuletin, eupafolin and kaempferol. K. brasiliensis at all doses and K. pinnata at doses of 250 mg/kg and 500 mg/kg significantly reduced the lesions in the ethanol induction model. In the indomethacin induction model, both species showed significant results at doses of 250 and 500 mg/kg. Also, the pre-treatment with leaf juices increased the antioxidant defense system, glutathione (GSH), whereas malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels were significantly decreased. Treatment with leaf juices led to the upregulation of zone occludes-1 (ZO-1) and the downregulation of inducible nitric oxide synthase (iNOS) and factor nuclear-κβ transcription (NF-κB-p65), while also showing a cytoprotective effect and maintaining mucus production. These findings show that the leaf juices of the two species showed gastroprotective effects on ethanol and gastric indomethacin injury which were a consequence of gastric inflammation suppression, antioxidant activity and the maintenance of cytoprotective defenses and mucosal structure architecture.

Section: Methodsmentioning

confidence: 99%

Gastroprotective and Antioxidant Activity of Kalanchoe brasiliensis and Kalanchoe pinnata Leaf Juices against Indomethacin and Ethanol-Induced Gastric Lesions in Rats

Guerra

et al. 2018

IJMS

“…Several recent pharmacological and phytochemical studies on these two plants have now laid down the scientific evidence for their traditional medicinal uses. These include antioxidant activity (Bashir and Gilani, 2009;Rajkumar et al, 2010), cytoprotective (Kakub and Gulfraz, 2007), antimicrobial (Rajbhandari et al, 2001;Sinha et al, 2001;Islam et al, 2002a), inhibition of digestive enzymes (Bhandari et al, 2008;Saijyo et al, 2008), hypoglycaemic effect (Islam et al, 2002b) and inhibition of calcium oxalate crystal formation and/or aggregation (Joshi et al, 2005;Bashir and Gilani, 2009). The other popular Bergenia species, B. crassifolia, is known to be used in Russian ethnomedicine as well as for beverages (Ivanov et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical, Antioxidant and Anti‐α‐glucosidase Activity Evaluations of Bergenia cordifolia

Roselli

Lentini

Habtemariam

2011

Phytotherapy Research

The antioxidant, anti-α-glucosidase and anticholinesterase activity of the leaves and rhizomatous extract of Bergenia cordifolia were investigated. The rhizomes extract that showed a higher degree of 1,1-diphenyl-2-picrylhydrazyl radical scavenging and anti-α-glucosidase activity than reference compounds (rutin and acarbose respectively) were subjected to phytochemical analysis. The study revealed that previously unknown minor constituents from the plant, (+)-catechin 3-O-gallate, (+)-catechin 3,5-di-O-gallate and 1,2,4,6-tetra-O-galloyl-β-D-glucopyranoside, were the radical scavenging and anti-α-glucosidase principles. These compounds as well as the crude extracts were weak acetylcholienesterase inhibitors, suggesting a higher degree of selectivity against α-glucosidase enzyme. In comparison with the minor constituents, the previously known major constituents of the plant, bergenin and arbutin, were poor radical scavengers and enzyme inhibitors.

“…The centrifuged gastric volume was measured from measuring the cylinder after transferring the supernatant liquid from centrifuge tubes [27]. The supernatant fluid was taken from the centrifuged tubes.…”

Section: Gastric Secretion Studymentioning

confidence: 99%

Anti-Ulcerogenic Effects of Salmalia Malabarica in Gastric Ulceration - Pilot Study

et al. 2015

Background. According to an estimation of the WHO, almost 80% of people globally are treated by traditional medicine. Objectives. We evaluated the anti-ulcerogenic potential of Salmalia malabarica extract in rats using aspirin-, alcohol-and pylorus ligation-induced ulcer models. Material and Methods. Two different doses (200 and 400 mg/kg body weight) of Salmalia malabarica extract was administered intraperitoneally (i.p.) to all 3 ulcer-induced models for 5 consecutive days. The anti-ulcerogenic potential in rats treated with 2 doses of Salmalia malabarica extract and omeprazole (20 mg/kg, i.p.) was determined and compared to the control groups. Results. Salmalia malabarica extract showed a significant decrease in ulcer index as compared to the control group in a dose-dependent manner. Salmalia malabarica extract also showed protection of 66.22% and 74.54% in asprin-, 73.79% and 78.14% in alcohol-and 68.94% and 78.84% in pylorus ligation-induced ulcers. However, omeprazole showed protection of 84.73%, 85.5% and 86.12% in aspirin-, alcohol-and pylorus ligation-induced ulcers, respectively. Furthermore, Salmalia malabarica extract significantly decreased the volume of gastric juice, free and total acidity, whereas it increased gastric pH when directly compared to the control group. Conclusions. Conclusively, Salmalia malabarica possesses anti-ulcerogenic, antisecretory, and cytoprotective potential and can be used as a supplement for the treatment of gastric ulcers in a dose dependent manner (Adv Clin Exp Med 2015, 24, 4, 595-605).