Cytoprotective effects of Glycyrrhizae radix extract and its active component liquiritigenin against cadmium-induced toxicity (effects on bad translocation and cytochrome c-mediated PARP cleavage)

Kim, Sang Chan; Byun, Sung Hui; Yang, Chae Ha; Kim, Chul Young; Kim, Jinwoong

doi:10.1016/j.tox.2004.01.010

Cited by 119 publications

(79 citation statements)

References 28 publications

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“…It has also been described in classical Asian medicine as an agent with the ability to 'improve the tone of the "middle-jiao" and replenish "qi", to remove "heat" and toxic substances and arrest coughing as well as the relief of spasms and pain [14]. In addition, it is widely used as a flavoring adjuvant in drug preparations and an ingredient of cigarettes for its taste and its properties that reduce irritation [14,15].…”

Section: Introductionmentioning

confidence: 99%

Effects ofGlycyrrhizae Radixon Repeated Restraint Stress-induced Neurochemical and Behavioral Responses

Park

Shim

Kim

et al. 2010

Korean J Physiol Pharmacol

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Glycyrrhizae radix (GR) is an herbal medicine that is commonly used in the East Asia for treating a variety of diseases, including stomach disorders. The objective of the present study was to examine the anti-stress effects of GR on repeated stress-induced alterations of anxiety, learning and memory in rats. Restraint stress was administered for 14 days (2 h/day) to the rats in the Control and GR groups (400 mg/kg/day, PO). Starting on the eighth day, the rats were tested for spatial memory on the Morris water maze test (MW ) and for anxiety on the elevated plus maze (EPM). W e studied the changes of the expressions of cholineacetyl transferase (ChAT) and tyrosine hydroxylase (TH) in the locus coerleus (LC) using immunohistochemistry. The results showed that the rats treated with GR had significantly reduced stress-induced deficits on their learning and memory on the spatial memory tasks. In addition, the ChAT immunoreactivities were increased. Gor the EPM, treatment with GR increased the time spent in the open arms (p＜ 0.001) as compared to that of the control group. Moreover, GR treatment also normalized the increases of the TH expression in the LC (p＜ 0.001).In conclusion, administration of GR improved spatial learning and memory and reduced stress-induced anxiety. Thus, the present results suggest that GR has the potential to attenuate the behavioral and neurochemical impairments caused by stress.

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Section: Introductionmentioning

confidence: 99%

Effects ofGlycyrrhizae Radixon Repeated Restraint Stress-induced Neurochemical and Behavioral Responses

Park

Shim

Kim

et al. 2010

Korean J Physiol Pharmacol

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“…ERβ is expressed in brain centers related to learning and memory and is less likely than ERα to be related to sexual function [14,15] . Second, liquiritigenin has been shown in several studies to exert cytoprotective effects [16,17] ; recent studies demonstrate that liquiritigenin exerts anti-inflammatory effects through the NF-κB pathway [18] . Third, we previously observed that liquiritigenin did not induce proliferation of MCF-7 and T47D breast cancer cells (data not shown), which is consistent with other studies [13] .…”

Section: Introductionmentioning

confidence: 99%

Liquiritigenin inhibits Aβ25–35-induced neurotoxicity and secretion of Aβ1–40 in rat hippocampal neurons

2009

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Aim: To examine whether liquiritigenin, a newly found agonist of selective estrogen receptor-β, has neuroprotective activity against β-amyloid peptide (Aβ) in rat hippocampal neurons. Methods: Primary cultures of rat hippocampal neurons were pretreated with liquiritigenin (0.02, 0.2, and 2 μmol/L) prior to Aβ 25-35 exposure. Following treatment, viability of the cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis and by a lactate dehydrogenase activity-based cytotoxicity assay. Intracellular Ca 2+ concentration ([Ca 2+ ] i ) and levels of reactive oxygen species (ROS), as well as apoptotic rates, were determined. Our studies were extended in tests of whether liquiritigenin treatment could inhibit the secretion of Aβ 1-40 as measured using an ELISA method. In order to analyze which genes may be involved, we used a microarray assay to compare gene expression patterns. Finally, the levels of specific proteins related to neurotrophy and neurodenegeration were detected by Western blotting. Results: Pretreated neurons with liquiritigenin in the presence of Aβ 25-35 increased cell viability in a concentration-dependent manner. Liquiritigenin treatment also attenuated Aβ 25-35 -induced increases in [Ca 2+ ] i and ROS level and decreased the apoptotic rate of neurons. Some genes, including B-cell lymphoma/leukemia-2 (Bcl-2), neurotrophin 3 (Ntf-3) and amyloid β (A4) precursor protein-binding, family B, member 1 (Apbb-1) were regulated by liquiritigenin; similar results were shown at the protein level by Western blotting. Conclusion: Our results demonstrate that liquiritigenin exhibits neuroprotective effects against Aβ 25-35 -induced neurotoxicity and that it can decrease the secretion of Aβ 1-40 . Therefore, liquiritigenin may be useful for further study as a prodrug for treatment of Alzheimer's disease.

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“…Therefore, two species are listed in pharmacopoeia worldwide. Licorice is one of the most important crude drugs prescribed with other herb medicines in traditional Chinese medicine (TCM) for the treatment and prophylaxis of sore throats (as an antitussive), coughs and bronchial catarrh (as an expectorant), inflammation, allergic reactions, rheumatism and arthritis, liver disease, tuberculosis and adrenocorticoid insufficiency [1][2][3][4][5]. Nearly 500 components have been identified in licorice.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, glycyrrhizin is well known as a natural sweetener and used in food additives and cosmetics [13]. On the other hand, flavonoids have also various pharmacological activities such as anti-hepatotoxic, anti-inflammatory, anti-ulcer, anti-allergenic and anti-viral as well as cardioprotective activities [2]. In order to control the quality of licorice, we investigated and succeeded the preparation of monoclonal antibody (MAb) against glycyrrhizin and set up an enzyme-linked immunosorbent assay (ELISA) as quick, simple and reproducible assay system [14].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Fertilization Activators for Future

Tanaka¹,

Wada²,

Tung³

et al. 2017

Biological Activities and Action Mechanisms of Licorice Ingredients

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Artificial insemination is an indispensable technology for cattle breeding and is used for treating infertility in humans. Thus, new or improved methods are needed to increase the efficiency of artificial insemination. In vitro fertilization (IVF) has been developed in mice. Although many mouse lines produced using IVF have been preserved by freezing embryos and/or fertilized eggs, the more efficient IVF using freezing preservation or long-term refrigeration of sperm is expected to preserve mouse lines more easily. In this chapter, we introduce the active compounds in licorice to improve the rate of IVF. We previously reported that the rate of IVF in mice was improved by adding a water extract of licorice (Glycyrrhiza uralensis), but not glycyrrhizin, to the artificial insemination culture medium. Recently, we analyzed the active ethyl acetate fraction containing high levels of flavonoids. This fraction was further purified by bioassay-guided separation to isolate isoliquiritigenin and formononetin, which contributed to the improved rate of IVF. Isoliquiritigenin and formononetin may be useful therapeutic agents for infertility treatment.

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Cytoprotective effects of Glycyrrhizae radix extract and its active component liquiritigenin against cadmium-induced toxicity (effects on bad translocation and cytochrome c-mediated PARP cleavage)

Cited by 119 publications

References 28 publications

Effects ofGlycyrrhizae Radixon Repeated Restraint Stress-induced Neurochemical and Behavioral Responses

Effects ofGlycyrrhizae Radixon Repeated Restraint Stress-induced Neurochemical and Behavioral Responses

Liquiritigenin inhibits Aβ25–35-induced neurotoxicity and secretion of Aβ1–40 in rat hippocampal neurons

In Vitro Fertilization Activators for Future

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