Cytoprotective effect of Fridericia chica (Bonpl.) L.G. Lohmann extract associated with geranylgeraniol enriched-fraction from Bixa orellana L. on epithelial cells treated with bisphosphonate

Abstract

Bisphosphonates are drugs used to treat bone disorders. The chronic use of bisphosphonates is associated with the occurrence of medication-related osteonecrosis of the jaw (MRONJ). Previous data reported the positive effects of Geranyl geraniol on different cell types treated with Bisphosphonates. Foregoing work done by our research group demonstrated the wound healing capacity of Fridericia chica (Bonpl.) L.G.Lohmann standardized ethanol extract. Herein in vitro cytoprotective synergistic effect of the association of F. chica extract associated with an enriched geranylgeraniol fraction on keratinocytes exposed to zoledronic acid is reported. An association of F. chica at 1 and 5µg/mL with geranylgeraniol at 15µg/mL, increased cell viability by 73.5% and 71.1%, respectively. This treatment did not increase tumor cells viability; whereas the clonogenic potential assessment showed that, the association with F. chica (5µg/mL) reversed the effects of zoledronic acid on the cells. This study provides data for a potential treatment for MRONJ.

One-step ultrasound-assisted recovery of yellow-orange-red natural coloring from defatted annatto seeds: A cleaner processing alternative

“…Despite being the main coloring compound in annatto colorants, bixin presents anti-inflammatory, antioxidant, anticancer, and skin-protecting activities that can add to the colorant's attractive bioactive functionalities [36] . Geranylgeraniol, on the other hand, has been studied for its biological activity, which includes action on suppression of proinflammation, modification of gut microbiome, and cytoprotective effect [37] , [38] .

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