Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes

Valentová, Kateřina; Ulrichová, Jitka; Cvak, Ladislav; Šimánek, Vilı́m

doi:10.1016/j.foodchem.2006.02.038

Cited by 62 publications

(57 citation statements)

References 21 publications

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“…The observed protective effects of the berry extracts against hydrogen peroxide-induced damage in rat neuronal cells is most likely due to the antioxidant properties of its constituents, mainly polyphenols [4]. Polyphenols are secondary metabolites present in vegetal tissues, flowers, and fruits.…”

Section: Discussionmentioning

confidence: 99%

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Protective Effects of Berry Extracts on Hydrogen Peroxide-induced Rat Brain Neuronal Cell Damage in vitro

Jeong¹,

Jang²,

Kum³

et al. 2014

JFNR

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The purpose of this study was to evaluate the antioxidative activities and protective effects of boysenberry, black currant, and blueberry extracts on neuronal cells. We found that berry extracts had different levels of antioxidant efficacy in various assays, including cell viability assays using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT). In addition, reactive oxygen species (ROS) were measured using 2',7'-dichlorofluorescein diacetate (DCF-DA) and flow cytometry analysis. Fruit extracts possessed strong antioxidant activities as measured using the malondialdehyde (MDA) assays. These extracts effectively inhibited lipid peroxidation. Black currant fruit extract significantly reduced intracellular ROS accumulation resulting from hydrogen peroxide treatment in PC12 cells. Black currant fruit more effectively inhibited lactate dehydrogenase (LDH) release into the medium than other tested fruit extracts did. The present results indicate that fruit extracts have the potential to protect neurons from hydrogen peroxide-induced cell damage and should be considered as a prospective functional food and therapeutic product.

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Section: Discussionmentioning

confidence: 99%

“…Various health benefits and disease prevention conferred by berry extracts have been attributed to large amounts of bioactive phytochemicals, including phenolic acids, anthocyanins, flavonols, and ellagitannins [4,5]. Many of the biological effects of phenolics are related to free radical scavenging and inhibition of cancer cell growth.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Berry Extracts on Hydrogen Peroxide-induced Rat Brain Neuronal Cell Damage in vitro

Jeong¹,

Jang²,

Kum³

et al. 2014

JFNR

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“…It belongs to a large genus (Vaccinium) of plants that also contain blueberry (Vaccinium corymbosum) and cranberry (Vaccinium macrocarpon). Bilberries are sometimes also called as blueberries because both have similar appearance and are close relatives, but the true blueberry is native to the United States (Valentova et al, 2006). Bilberry usually grows in heaths, meadows and moist coniferous forests, and its growth is favoured by moderate shade and moderately humid ground conditions.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and Bioactive Characteristics of Wild Grown Bilberry (Vaccinium myrtillus L.) Genotypes from Northeastern Turkey

Çelik

Bozhuyuk

Erċışlı

et al. 2018

Not Bot Horti Agrobo

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In the present study, fruit weight, fruit firmness, external fruit color, soluble solid content, pH, titratable acidity, vitamin C, total phenolics, total anthocyanins and antioxidant capacity of eight wild bilberry grown in Coruh valley in northeastern Turkey were determined. The blueberry cv. 'Bluecrop' was also studied to make comparison with bilberry genotypes. Antioxidant activity of bilberry and blueberry fruits were comparatively assessed by FRAP (Ferric reducing antioxidant power) assay. Significantly higher contents of phenolics and anthocyanins were found in bilberry fruits comparted with blueberry fruits. However, blueberry cv. 'Bluecrop' exhibited higher fruit weight and vitamin C content than bilberry fruits. Total phenolic and total anthocyanin content was 327 mg gallic acid equivalent and 142 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruit in cv. 'Bluecrop', while it was between 492-563 mg gallic acid equaivalent and 307-342 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruits of bilberry accessions. Moreover, wild accessions had approximately 2-3 folds higher antioxidant capacity than cv. 'Bluecrop'. The obtained results suggest the possibility of improving the bioactive and antioxidant properties of bilberry cultivars by using wild ones in cross breedings. It was also concluded that genotypes significantly affect the bioactive content of fruits and consequently the possibility of using wild bilberry fruits as a potential source of natural antioxidants in food industry is worth to be considered.

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“…Therefore, it is proposed that BB has a protective ef- bilberry fruit on the selected cells. Valentová et al (2007) also reported that the bilberry extract showed a significant (P < 0.01) dose-dependent (100 and 500 µg DM/ml) protective activity against oxidative damage (induced by tert-butyl hydroperoxide and allyl alcohol) in primary cultures of rat hepatocytes. Additionally, Schantz et al (2010) observed a significant (P < 0.05) reduction of menadione-induced DNA damage (6 µM, 1 h) in the Caco-2 cells after pretreatment with a bilberry extract (5 and 50 µg/ml, 24 h).…”

Section: Resultsmentioning

confidence: 88%

“…Then, a daily intake of bilberry extract (100 and 400 ppm extract in drinking water) was also recommended to suppress the hepatocarcinogenic process in piperonyl butoxide-promoted hepatocarcinogenesis in rats (Hara et al 2014). Last but not least, Valentová et al (2007) showed no cytotoxic effect of the bilberry concentrate in the MTT assay (100 and 500 µg DM/ml; 4, 24, or 48 h) on primary cultures of rat hepatocytes. However, the present work has been the first attempt to examine the potential cytotoxicity of the whole bilberry fruit, not just an anthocyanin-rich bilberry extract.…”

Section: Resultsmentioning

confidence: 92%

Effects of a bilberry preparation on selected cell lines of the digestive system

Borowiec¹,

Szwajgier²,

Olejnik³

et al. 2016

Czech J. Food Sci.

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Borowiec K., Szwajgier D., Olejnik A., Kowalska K., Targoński Z. (2016): Effects of a bilberry preparation on selected cell lines of the digestive system. Czech J. Food Sci., 34: 300-305.Bilberry is a valuable wild forest fruit harvested in many countries in Europe. The biological activities of bilberry include antioxidant, anticancer, antiviral, antibacterial, and anticholinesterase activities. This study examines the protective effects of a bilberry (BB) preparation on IEC-6, Caco-2, and HepG2 cell lines. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to study the cytotoxicity of BB. The genotoxicity was determined using single-cell microgel electrophoresis. The Ames test was employed to assay bilberry mutagenicity. No significant effects of BB (12.5-100 µg dry mass/ml) were observed on the viability of IEC-6, Caco-2, and HepG2 cells. There were no differences in the percentage of DNA in the comet tail between the cells treated with BB (100 µg dry mass/ml) and the control cells. However, a significant reduction of oxidative DNA damage in the HepG2 cells was found. BB exhibited neither mutagenic nor promutagenic effects. Our results suggest that bilberry can be a potential tool in the prevention of chronic diseases, without any undesired effects on the cells of the gastrointestinal tract.

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Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes

Cited by 62 publications

References 21 publications

Protective Effects of Berry Extracts on Hydrogen Peroxide-induced Rat Brain Neuronal Cell Damage in vitro

Protective Effects of Berry Extracts on Hydrogen Peroxide-induced Rat Brain Neuronal Cell Damage in vitro

Physicochemical and Bioactive Characteristics of Wild Grown Bilberry (Vaccinium myrtillus L.) Genotypes from Northeastern Turkey

Effects of a bilberry preparation on selected cell lines of the digestive system

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