Cytoprotective effect of 16,16' dimethyl prostaglandin E2 (dm PGE2) on streptozotocin-induced biochemical alterations of Golgi-rich membrane fraction in comparison with morphology of rat liver Golgi apparatus in situ

Kordowiak, Anna M

doi:10.1016/s0344-0338(86)80074-7

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…Insulin, the main metabolic hormone, acting inter cilia on sugar conversion, did not normalize detected biochemical alterations, only slightly influenced the morphology of Golgi apparatus as shown in experiments in situ (4).The second drug investigated by us, 16,16 dimethyiprostaglandin E2, could l)erfectlY prevent both biochemical and morphological alterations within the Golgi area. It did not act as a "medicine" but as a cytoprotective drug, as it had to be applied prior to, concomitantly with and after injection of SZ to be efficacious (25,26).…”

Section: Discussionmentioning

confidence: 99%

“…In our previous investigations, major biochemical and morphological alterations were found on the cell level in streptozotocin diabetic rat liver (4,5). These changes were completely prevented by prostaglandin E2 (25,26), but only slightly influenced by insulin (4,5). It seemed interesting to study insulin-like effect a new vanadium complex (i.e.…”

Section: Introductionmentioning

confidence: 99%

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Insulin-Like Effects on Liver Golgi Membrane Preparations of Bis(Oxalato)Oxovanadate(IV) Complex Ion, a New Vanadate Compound

Kordowiak

Trzos²,

Gryboś³

1997

Horm Metab Res

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Recent studies have shown the insulin-like effect of vanadyl sulphate or sodium ortho (or meta-)vanadate administered orally to rats. Toxicity of these drugs and reluctance by the animals to drink the solutions and take food, concerning the amelioration of some diabetes syndrome discussed in 1994 by Domingo et al. (1), McNeill et al. (2) and Wiliams and Malabu (3), prompted us to investigate a new vanadate complex: disodium bis(oxalato)oxovanadate (IV), Na2[VO(OX)2]H2O. The main object of the experiment was to study whether this complex administered as 3 mmol/l solution in 0.5% NaCl during 7 days could act on the subcellular level and influence the activity of liver Golgi membrane galactosyltransferase activity. Free blood sugar level was lowered (but was still higher than in the control group) in diabetic rats after seven days of vanadate action and was accompanied by lowered, however not statistically significant, serum triglyceride levels. The yields of isolated Golgi-rich membrane fractions were about half of the level in diabetic groups (untreated and treated with vanadium) compared with the control groups. Purity of these membrane fractions, expressed as nmol Gal transferred per mg of proteins and per h, was the same in four groups investigated and showed the possibility to compare them. Activity of galactosyltransferase calculated in nmol Gal transferred per 1 g of liver and per 1 h or per whole liver in the same time (as a possibility of glycosylation of the secretory and membrane glycoproteins) was lower in both diabetic groups. However, after vanadium treatment (D+V group), the activity was higher than in untreated diabetic rats (D group) in three of five investigated animals. Vanadyl-oxalate complex did not normalize in a statistically significant manner the enzyme activity which was significantly lower in diabetes than in control. This is similar to insulin influence on the galactosyltransferase activity reported previously by Kaczmarski et al. in 1981 (4) and Kordowiak et al. in 1981 (5).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Insulin-Like Effects on Liver Golgi Membrane Preparations of Bis(Oxalato)Oxovanadate(IV) Complex Ion, a New Vanadate Compound

Kordowiak

Trzos²,

Gryboś³

1997

Horm Metab Res

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“…In our laboratory, such characteristic alterations of the biochemical activity and morphology of liver Golgi complexes in situ were previously found in untreated, streptozotocin (STZ)-induced diabetes in rats [22,23], so they served as a convenient indicator of the effectiveness of tested complexes such as normalising vanadium [13 -15,19, 20] as well as cytoprotective, non-vanadium derivatives [24,25], and used in a study on bis(2,2'-bipyridine)oxovanadium(IV) sulphate [VO(bpy) 2 ], a new synthetic vanadium complex.…”

mentioning

confidence: 99%

The Influence of a New Vanadium Compound, Bis(2,2’-bipyridine)oxovanadium(IV) Sulphate on Liver Golgi Complexes from Control and Streptozotocin-Diabetic Rats

Am¹,

Dabroś²,

B³

2002

Horm Metab Res

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Among the previously studied organic vanadium derivatives showing an anti-diabetic action, we investigated a new complex, bis(2,2'-bipyridine)oxovanadium(IV) sulphate. We tested its ability to normalise parameters previously described for streptozotocin (STZ)-diabetes, such as lower yields of Golgi-rich membrane fraction isolation, decreased activity of Golgi membrane marker enzyme - galactosyltransferase (GalT) - and altered morphology of rat liver Golgi complexes. Oral application as a drinking solution of 1.8 mmol bis(2,2'-bipyridine)oxovanadium(IV) (dissolved in 0.09 M NaCl) caused a similar dispersion of GalT activities in both vanadium treated groups, control and diabetic. Very low activities of the enzyme (characteristic for untreated diabetes) we found only in approximately 35 % of the STZ-diabetic rats treated with the new vanadium compound. The morphology of liver Golgi complexes in diabetic rats treated with bis(2,2'-bipyridine)oxovanadium(IV) sulphate was improved, which manifested itself in the reappearance of vacuoles with VLDL and coated and uncoated secretory vesicles. In view of biochemical and morphological parameters of normalised diabetic rat liver Golgi apparatus, the new vanadium complex was more effective than bis(oxalato)oxovanadium(IV) or bis(kojato)oxovanadium(IV), but in our experimental model, the best anti-diabetic, orally applied drug was the bis(maltolato)oxovanadium(IV) previously investigated.

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Sodium orthovanadate exerts influence on liver Golgi complexes from control and streptozotocin-diabetic rats

Kordowiak

Goc

Drozdowska

et al. 2005

Journal of Inorganic Biochemistry

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Cytoprotective effect of 16,16' dimethyl prostaglandin E2 (dm PGE2) on streptozotocin-induced biochemical alterations of Golgi-rich membrane fraction in comparison with morphology of rat liver Golgi apparatus in situ

Cited by 6 publications

References 9 publications

Insulin-Like Effects on Liver Golgi Membrane Preparations of Bis(Oxalato)Oxovanadate(IV) Complex Ion, a New Vanadate Compound

Insulin-Like Effects on Liver Golgi Membrane Preparations of Bis(Oxalato)Oxovanadate(IV) Complex Ion, a New Vanadate Compound

The Influence of a New Vanadium Compound, Bis(2,2’-bipyridine)oxovanadium(IV) Sulphate on Liver Golgi Complexes from Control and Streptozotocin-Diabetic Rats

Sodium orthovanadate exerts influence on liver Golgi complexes from control and streptozotocin-diabetic rats

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