Cytoprotective and antioxidant activity of Rhodiola imbricata against tert-butyl hydroperoxide induced oxidative injury in U-937 human macrophages

Abstract

The present study reports cytoprotective and antioxidant activity of aqueous and alcoholic extracts of Rhodiola imbricata rhizome on tert-butyl hydroperoxide (tert-BHP) induced cytotoxicity in U-937 human macrophages. There was an increase in cytotoxicity and apoptosis significantly in the presence of tert-BHP over control cells. The tert-BHP induced cytotoxicity can be attributed to enhanced reactive oxygen species (ROS) production which in turn is responsible for fall in reduced glutathione (GSH) levels; further there was a significant decrease in mitochondrial potential and increase in apoptosis and DNA fragmentation. Both aqueous and alcoholic extracts of Rhodiola rhizome at a concentration of 250 microg/ml were found to inhibit tert-BHP induced free radical production, apoptosis and to restore the anti-oxidant levels to that of the control cells. The alcoholic extract of Rhodiola showed higher cytoprotective activities than aqueous extract. These observations suggest that the alcoholic and aqueous extracts of Rhodiola have marked cytoprotective and antioxidant activities.

Effects of <i>Salidroside</i> on Cobalt Chloride-Induced Hypoxia Damage and mTOR Signaling Repression in PC12 Cells

“…1a), has been documented to possess neuroprotective effects against neuronal damage induced by various insults. [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] However, the underlying mechanisms are still not well understood.…”

“…11,12) Salidroside (SA), a natural compound from Rhodiola rosea L., is a major active ingredient responsible for most pharmacological effects of Rhodiola, such as anti-oxidative, anti-aging, anti-inflammatory, anti-cancer, anti-fatigue and anti-depressant activities. [13][14][15][16][17][18][19] Increasing evidence suggests that SA may have neuroprotective effects in injured brain. SA can reduce the degree of cerebral edema and the brain infarct size of rats with global cerebral ischemia, relieve the metabolism abnormity of free radicals and improve the function of cognition as well as behavioral and histological outcomes.…”

Effect of Temperature on in Vitro Organogenesis of Rhodiola Imbricata Edgew. – A Medicinal Herb

“…RI1, RI3, and RI7 at 15 ± 2°Cand 25± 2°C. Shoot multiplication was observed earlier at 15 ± 2°C (18)(19)(20)(21)(22)(23) days) compared to 25± 2°C (25-30 days). Also, at 15 ± 2°C, number and length of shoots were found to be much higher (Graph 1).The MS medium supplemented with BAP (1 mg/l) + KN (2 mg/l) was found to be the best for the multiplication of shoots in 18 -23 days with …”

Evaluación del efecto citotóxico y del daño genético de extractos estandarizados de Solanum nudum con actividad antiplasmodial

“…Se evaluaron cuatro concentraciones de los extractos, siendo la máxima CC 50 la obtenida en los ensayos de citotoxicidad para la misma línea celular (cuadro 2). Se colocaron 150.000 células en contacto con los tratamientos y controles (28)(29). Como control negativo se utilizó DMSO al 0,5 % y, como controles positivos, peróxido de hidrógeno (H 2 O 2 ) a 100 µM sin activación metabólica (fracción S9) y 2-aminofluoreno, a 1 µM, para la activación metabólica.…”

“…Los tratamientos se incubaron durante una a tres horas y se midió la viabilidad de las células después del tratamiento (28)(29)(30). Se tomaron 20 µl de las células, las cuales se mezclaron con 80 µl de agarosa de bajo punto de fusión (0,5 %) y la mezcla se colocó en placas portaobjetos, previamente impregnadas con agarosa de punto de fusión normal (1 %).…”