Cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extracts on the mouse embryonic fibroblast cell

Ha, Ju Yeon; Lee, Kyueun; Park, Jeongmi; Dong, Ahyoung; Shin, Han‐Seung

doi:10.1007/s10068-010-0167-y

Cited by 10 publications

(5 citation statements)

References 11 publications

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“…Catechin, quercetin, quercetin-3- O -glycoside, kampherol-3- O -glycoside, and myricetin-3- O -glucoside have been reported as part of its major components ( 6 – 8 ). Pharmacologic and physiological activities including antidiabetic, cytoprotective, antibacterial, antioxidant, and antiobesity effects have been documented from the extracts of lotus leaves, seed, and rhizome ( 4 , 6 , 7 , 9 , 10 ). However, limited information is available with regard to the evaluation of its antioxidant activity on cells, specifically on human hepatocyte.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Cytoprotective Effects of Lotus (Nelumbo nucifera) Leaves Phenolic Fraction

Lee¹,

Kim²,

Je³

2015

JFN

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Phenolic rich ethyl acetate fraction (EAF) from lotus leaves was prepared and its bioactive components, antioxidant and cytoprotective effects were investigated. EAF showed high total phenolic content and flavonoid content and contained rutin (11,331.3±4.5 mg/100 g EAF), catechin (10,853.8±5.8 mg/100 g EAF), sinapic acid (1,961.3±5.6 mg/100 g EAF), chlorogenic acid (631.9±2.3 mg/100 g EAF), syringic acid (512.3±2.5 mg/100 g EAF), and quercetin (415.0±2.1 mg/100 g EAF). EAF exerted the IC50 of 4.46 μg/mL and 5.35 μg/mL toward DPPH and ABTS cation radicals, respectively, and showed strong reducing power, which was better than that of ascorbic acid, a positive control. Additionally, EAF protected hydroxyl radical-induced DNA damage indicated by the conversion of supercoiled pBR322 plasmid DNA to the open circular form and inhibited lipid peroxidation of polyunsaturated fatty acid in a linoleic acid emulsion. In cultured hepatocytes, EAF exerted a cytoprotective effect against oxidative stress by inhibiting intracellular reactive oxygen species formation and membrane lipid peroxidation. In addition, depletion of glutathione under oxidative stress was remarkably restored by treatment with EAF. The results suggest that EAF have great potential to be used against oxidative stress-induced health conditions.

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Section: Introductionmentioning

confidence: 99%

Antioxidant and Cytoprotective Effects of Lotus (Nelumbo nucifera) Leaves Phenolic Fraction

Lee¹,

Kim²,

Je³

2015

JFN

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“…Aqueous extracts from the leaves of two olive tree varieties (Meski and Chemlali) proved to increase the viability of murine oligodendrocytes (158N) in the concentration range of 5-200 μg/mL, while at 400 μg/mL, the viability of 158N was reduced [26]. The olive leaf ethanolic extracts also suppressed cytotoxicity induced by oxidative stress on the mouse embryonic fibroblast (MEF) [27]. Hydroethanolic extract from the leaves of the Chemlali cultivar proved to inhibit the proliferation of a cell line derived from human chronic myeloid leukemia (K562) and to exert Luteolin-7-O-glucoside At this stage of analysis, it is noteworthy that the extracts obtained from the underground parts of the olive tree Chemlalai cultivar exhibit no significant cytotoxicity towards non-cancerous VERO cells.…”

Section: Cytotoxicity and Antiviral Activitymentioning

confidence: 99%

Comprehensive Metabolite Profiling of Chemlali Olive Tree Root Extracts Using LC-ESI-QTOF-MS/MS, Their Cytotoxicity, and Antiviral Assessment

Toumi,

Świątek,

Boguszewska

et al. 2023

Molecules

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The large quantity of olive roots resulting from a large number of old and unfruitful trees encouraged us to look for ways of adding value to these roots. For this reason, the current research work is devoted to the valorization of olive roots by identifying active phytochemicals and assessing their biological activities, including the cytotoxicity and antiviral potential of different extracts from the Olea europaea Chemlali cultivar. The extract, obtained by ultrasonic extraction, was analyzed using the liquid chromatography-mass spectrometry technique (LC-MS). The cytotoxicity was evaluated through the use of the microculture tetrazolium assay (MTT) against VERO cells. Subsequently, the antiviral activity was determined for HHV-1 (Human Herpesvirus type 1) and CVB3 (Coxsackievirus B3) replication in the infected VERO cells. LC-MS analysis allowed the identification of 40 compounds, classified as secoiridoids (53%), organic acids (13%), iridoids (10%), lignans (8%), caffeoylphenylethanoid (5%), phenylethanoids (5%),sugars and derivatives (2%), phenolic acids (2%), and flavonoids (2%). It was found that extracts were not toxic to the VERO cells. Moreover, the extracts did not influence the appearance of HHV-1 or CVB3 cytopathic effects in the infected VERO cells and failed to decrease the viral infectious titer.

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“…Considerable literatures have demonstrated that lotus leaf contains nuciferine, quercetin, quercetin-3-Oglycoside, kampherol-3-O-glycoside, and myricetin-3-O-glucoside [16]. These compounds from lotus leaves, seed and rhizome display cytoprotective, anti-bacterial, anti-obesity especially anti-oxidant pharmacologic activities [17][18][19][20][21]. However, the effect of lotus leaf extract on cancer metastasis still remained unclear.…”

Section: Introductionmentioning

confidence: 99%

Lotus leaf extract inhibits ER- breast cancer cell migration and metastasis

Tong

et al. 2021

Preprint

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Background: Patients with estrogen receptor negative (ER-) breast cancer have poor prognosis due to high rates of metastasis. However, there is no effective treatment and drugs for ER- breast cancer metastasis. Our purpose of this study was to evaluate the effect of lotus leaf alcohol extract (LAE) on the cell migration and metastasis of ER- breast cancer.Methods: The anti-migratory effect of LAE were analyzed in ER- breast cancer cells including SK-BR-3, MDA-MB-231 and HCC1806 cell lines. Cell viability assay, wound-healing assay, RNA-sequence analysis and immunoblotting assay were used to evaluate the cytotoxicity and anti-migratory effect of LAE. To further investigate the inhibitory effect of LAE on metastasis in vivo, subcutaneous xenograft and intravenous injection nude mice models were established. Lung and liver tissues were analyzed by the Hematoxylin & eosin staining and immunoblotting assay. Results: We found that lotus leaf alcohol extract (LAE), not nuciferine, inhibited cell migration significantly in SK-BR-3, MDA-MB-231 and HCC1806 breast cancer cells, and did not affect viability of breast cancer cells. The anti-migratory effect of LAE was dependent on TGF-β1 signaling, while independent of Wnt signaling and autophagy influx. Intracellular H2O2 was involved in the TGF-β1-related inhibition of cell migration. LAE inhibited significantly the breast cancer cells metastasis in mice models. RNA-sequence analysis showed that extracellular matrix signaling pathways are associated with LAE-suppressed cell migration. Conclusions: Our findings demonstrated that lotus leaf alcohol extract inhibits the cell migration and metastasis of ER- breast cancer, at least in part, via TGF-β1/Erk1/2 and TGF-β1/SMAD3 signaling pathways, which provides a potential therapeutic strategy for ER- breast cancer.

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Cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extracts on the mouse embryonic fibroblast cell

Cited by 10 publications

References 11 publications

Antioxidant and Cytoprotective Effects of Lotus (Nelumbo nucifera) Leaves Phenolic Fraction

Antioxidant and Cytoprotective Effects of Lotus (Nelumbo nucifera) Leaves Phenolic Fraction

Comprehensive Metabolite Profiling of Chemlali Olive Tree Root Extracts Using LC-ESI-QTOF-MS/MS, Their Cytotoxicity, and Antiviral Assessment

Lotus leaf extract inhibits ER- breast cancer cell migration and metastasis

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