Cytoplasmic RNA sensors and their interplay with RNA-binding partners in innate antiviral response: theme and variations

Chan, Chi-Ping; Jin, Dong Yan

doi:10.1261/rna.079016.121

Cited by 19 publications

(14 citation statements)

References 344 publications

(504 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The RLRs Ifih1/MDA5 and Ddx58/RIG-I function as cytoplasmic sensors of viral RNA [ 45 ] and have anticancer roles [ 46 ]. Ifih1/MDA5 mRNA was upregulated after poly(I:C) transfection of each cell type; however, Ddx58/RIG-I mRNA, although present, was not upregulated.…”

Section: Discussionmentioning

confidence: 99%

Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells

et al. 2022

View full text Add to dashboard Cite

Germline-encoded pattern recognition receptors [PRRs] in mammalian cells function in the detection of molecular patterns associated with pathogen invasion or cellular damage. A PRR subset is activated by the atypical presence and location of double-stranded RNA [dsRNA] or its synthetic analogue polyinosinic-polycytidylic acid [poly(I:C)], triggering pro-inflammatory signalling and death in many cell types. Poly(I:C) has been tested as a sole or combination cancer therapy in preclinical studies and clinical trials. The purpose of this study was to evaluate the effects of poly(I:C) transfection via electroporation on cell lines from a cancer of epithelial origin, 4T1 mammary carcinoma, and a cancer of mesenchymal origin, WEHI 164 fibrosarcoma. The effects of the poly(I:C) delivery on cell metabolism implicate the induction of cell death. A pro-inflammatory response was demonstrated by mRNA upregulation and the secretion of Type I interferon and several cytokines and chemokines. The mRNAs of dsRNA sensor DExD/H-box helicase 58/retinoic acid-inducible gene I protein [Ddx58/RIG-I] and sensor/co-sensor DEAH-box helicase 9 [Dhx9] were not regulated, but the mRNAs of RNA sensors toll-like receptor 3 [TLR3], interferon-induced with helicase C domain 1/melanoma differentiation-associated protein 5 [Ifih1/MDA5] and Z-DNA binding protein 1 [Zbp1] and co-sensors DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 [Ddx60] and interferon-inducible protein 204 [Ifi204] were upregulated in both cell lines. The mRNAs encoding signalling pathways components were present or upregulated in both cell types. These data demonstrate that RNA sensing effects can be amplified by electroporation delivery, potentially expanding the practicality of this immunotherapeutic approach.

show abstract

Section: Discussionmentioning

confidence: 99%

Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Besides, some emerging RNA sensors have been revealed and defined, including NOD-like receptors (NLRs), heterogeneous nuclear ribonucleoproteins (hnRNPs), DEAD-box or DEAH-box RNA helicases and ZBP1 [ 5 , 10 ]. These RNA sensors are found to sense RNA and interact with TLRs and RLRs in innate immunity.…”

Section: Rna Sensors Contribute To Cancer Immunitymentioning

confidence: 99%

“…When combined with RNA, the RNA sensors of TLRs switch through conformational changes, recruit adaptor protein TRIF for TLR3 and MyD88 for TLR7/TLR8, causing IRF3/7 phosphorylation and IFN-β secretion [ 9 ]. RLRs predominately localize to the cytoplasm and consist of three members: RIG-I, melanoma differentiation-associated protein 5 (MDA5), and laboratory of genetics and physiology 2 (LGP2) [ 10 – 12 ]. RIG-I recognizes 5’-triphosphate RNA (3pRNA) in the cytoplasm of virus-infected cells, and initiates antiviral immune response by activating MAVS and TBK1 signals [ 13 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Emerging role of RNA sensors in tumor microenvironment and immunotherapy

Yang

et al. 2022

J Hematol Oncol

View full text Add to dashboard Cite

RNA sensors detect foreign and endogenous RNAs to protect the host by initiating innate and adaptive immune response. In tumor microenvironment (TME), activation of RNA sensors induces tumor-inhibitory cytotoxic T lymphocyte responses and inhibits the activity of immunosuppressive cells though stimulating type I IFN signaling pathway. These characteristics allow RNA sensors to be prospective targets in tumor immunotherapy. Therefore, a comprehensive understanding of the roles of RNA sensors in TME could provide new insight into the antitumor immunotherapy. Moreover, RNA sensors could be prominent triggering targets to synergize with immunotherapies. In this review, we highlight the diverse mechanisms of RNA sensors in cancer immunity and their emerging contributions in cancer immunotherapy, including monotherapy with RNA sensor agonists, as well as combination with chemotherapy, radiotherapy, immune checkpoint blockade or cancer vaccine.

show abstract

“…RIG-I can sense the non-self RNA in the cytoplasm, like viral RNA. When non-self RNA is detected, RIG-I changes its original self-inhibitory conformation into activated conformation [5,6]. Mitochondrial antiviral signaling protein (MAVS) subsequently interacts with RIG-I and transduces the signaling to IRF-3/IRF-7 and NF-κB.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Retinoic Acid-Inducible Gene I Overexpression Reveals the Potential Genes for Autophagy-Related Negative Regulation

Tan

Yang

et al. 2022

Cells

View full text Add to dashboard Cite

Retinoic acid-inducible gene I (RIG-I) serves as an essential viral RNA sensor for innate immune. The activation of the RIG-I-like receptors (RLRs) pathway triggers many regulations for the outcome of type I interferon, including ubiquitination, dephosphorylation, ISGylation, and autophagy. However, the autophagy-related regulation of RIG-I is still not fully understood. To investigate the potentially unknown genes related to autophagy-related regulation of RIG-I, we firstly confirm the induction of autophagy derived by overexpression of RIG-I. Furthermore, the autophagy inducer and inhibitor drugs were used in different assays. The results showed autophagy could control the activation of RLRs pathway and expression of exogenous RIG-I. In addition, we carried out the transcriptome analysis of overexpression of RIG-I in vitro. Differentially expressed genes (DEGs) in GO and KEGG signaling pathways enrichment provided a newly complex network. Finally, the validation of qPCR indicated that the DEGs PTPN22, PRKN, OTUD7B, and SIRT2 were correlated to the negative regulation of excessive expression of RIG-I. Taken together, our study contributed new insights into a more comprehensive understanding of the regulation of excessive expression of RIG-I. It provided the potential candidate genes for autophagy-related negative regulation for further investigation.

show abstract

Cytoplasmic RNA sensors and their interplay with RNA-binding partners in innate antiviral response: theme and variations

Cited by 19 publications

References 344 publications

Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells

Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells

Emerging role of RNA sensors in tumor microenvironment and immunotherapy

Transcriptome Analysis of Retinoic Acid-Inducible Gene I Overexpression Reveals the Potential Genes for Autophagy-Related Negative Regulation

Contact Info

Product

Resources

About