Cytoplasmic retention of p-Erk1/2 and nuclear accumulation of actin proteins during cellular senescence in human diploid fibroblasts

Reduction of exportin 6 activity leads to actin accumulation via failure of RanGTP restoration and NTF2 sequestration in the nuclei of senescent cells

“…Primary culture of human diploid fibroblast (HDF) was prepared in our laboratory [7,8,31] and maintained in Dulbecco's modified Eagle's medium (DMEM-high glucose, GIBCO BRL, Grand Island, N.Y.) supplemented with 10% heat inactivated fetal bovine serum (FBS, GIBCO BRL). A number of population doublings and doubling times (DT) of HDF were calculated based on the equation, described previously [32].…”

“…induced senescence, whereas inhibition of cell growth prevents cellular senescence when the cell cycle is blocked by DNA damage and p21 WAF1 [6]. We earlier reported that globular actin (G-actin) accumulates in nuclei of the replicatively senescent human diploid E X P E R I M E N T A L C E L L R E S E A R C H 3 1 7 ( 2 0 1 1 ) 9 4 1 -9 5 4 fibroblasts (HDF) and induced senescence of cancer cells by treatment with either H 2 O 2 , deferoxamine, TGF-β1 or over expression of H-Ras double mutants [7][8][9].…”

Nuclear Factor–Erythroid-2–Related Factor 2 in Aging and Lung Fibrosis

“…63 Alterations in actin cytoskeletal regulation with aging would be expected to contribute to dysregulated Nrf2 responses. 64 Further studies of Nrf2 activation and cytoskeletal regulation in aging are warranted.…”

Loss of p21Sdi1 expression in senescent cells after DNA damage accompanied with increase of miR-93 expression and reduced p53 interaction with p21Sdi1 gene promoter

“…Primary culture of HDF was prepared in our laboratory from foreskin of 4 year-old boy [15,[20][21][22] and maintained in DMEMhigh glucose supplemented with 10% heat inactivated fetal bovine serum under 5% CO 2 in air. To evaluate characteristics of in vivo cellular senescence, skin fibroblasts were freshly isolated from buttock of 24-75 year-old men and cultivated until use.…”