Cytoplasmic Protein Synthesis in Mouse Brain

Herriman, I. D.; Hunter, G. D.

doi:10.1111/j.1471-4159.1965.tb11937.x

Cited by 27 publications

(12 citation statements)

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“…T h e technique of in vivo incorporation used in this study ensured a relatively good labeling of cerebral man & Hunter, 1965;Jacob et al, 1966;Vesco & Giuditta, 1967). T h e differentiation and identification of mRNA from precursors of ribosomal RNA by sedimentation technique is still questionable.…”

Section: Discussionmentioning

confidence: 97%

“…the brain of rats (Appel, 1967;Jacob, Stevenin, Jund, Judes & Mandel, 1966;La Torre & Tandler, 1967;Nievel & Kirby, 1966), mice (Herriman & Hunter, 1965) and rabbits (Vesco & Giuditta, 1967). A series of properties of messenger RNA (mRNA) such as the incorporation of labelled precursors into the structure of mRNA, the base content, and the template activity manifesting itself by the stimulation of protein synthesis in cell-free system, was investigated.…”

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confidence: 99%

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Synthesis of rapidly‐labelled brain RNA in the rat during stagnant hypoxia in the course of ontogeny

Sirakova

Sirakov

Jílek

et al. 1968

Developmental Psychobiology

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SIRAKOVA, I. A., SIRAKOV, L. M., J~L E K , L., and RYCHL~K, I. (19138). Synthesis of Rapidly-lnbelled Brain R N A in the R a t During StagizantHypoxia in the Course of Ontogeny. DEVELOPMENTAL PsucilosroLocu, I(3): 189-195. T h e nucleic acids of the cytoplasmic and nuclear fraction of the brain honiogenate were isolated by phenol procedure then were fractionated on a linear sucrose density gradient. T h e in vivo incorporation of [3H]-uridine into different types of RNA and the template activity of these nucleic acid fractions in control and hypoxic animals were studied in vitro in a cell-free protein synthetic system of E. coli. In adult animals, as in 25-day-old rats, stagnant hypoxia decreases the total synthesis of rapidly-labelled RNA in nuclei and impairs the template activity in cytoplasm. In 12-day-old rats, the synthesis of rapidly-labelled RNA does not change in stagnant hypoxia and the template activity increases mainly in the low molecular weight fractions. Metabolic adaptation of immature nervous tissue to hypoxia involves specific changes of protein and nucleic acid metabolism as well as in carbohydrate and energy metabolism.

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Section: Discussionmentioning

confidence: 97%

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confidence: 99%

Synthesis of rapidly‐labelled brain RNA in the rat during stagnant hypoxia in the course of ontogeny

Sirakova

Sirakov

Jílek

et al. 1968

Developmental Psychobiology

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“…However, when rat liver RNA was added as carrier, a large proportion of the labelled RNA was found in the denser regions of the gradient and there is little doubt that considerable aggregation had taken place. HERRIMAN and HUNTER (1965) studied the biological activity of mouse brain RNA which was fractionated on columns of methylated serum albumin. They found that a considerable amount of biologically active RNA was eluted by 0.6 M-NaC1, which is equivalent to RNA of a size less than 16s.…”

Section: Discussionmentioning

confidence: 99%

“…The ability of RNA fractions to stimulate the incorporation of [l4C] amino acids into protein was measured using the following system. Mouse brain ribosomes and the particle free supernatant fractions were prepared according to the method described by HERRIMAN and HUNTER (1965). About 500 pg of brain ribosomes (01 ml) were incubated with 0.3 ml of the 100,000 g supernatant fraction (800-1100 pg of protein); 0.7 ml of a solution containing 50 pmoles KCl, 100 pmoles tris-HCI, pH 7.4, 5 pmoles MgCl,, 20 pmoles GSH, 1 pmole GTP, 2 pmoles ATP, 10 pmoles phosphoenolpyruvate; 0.1 ml [2-14C]valine or leucine (500 mpc, 0.38 p g ) and 0.05 ml pyruvate kinase (0.1 mg).…”

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Rna Synthesis in Mouse Brain

Kimberlin

1967

Journal of Neurochemistry

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SEVERAL reports have shown that brain is an active site of protein synthesis. In particular, microsomal and ribosomal preparations have been obtained which will incorporate amino acids into protein and it appears that these preparations are comparable in activity with those derived from hepatic tissue (BONDY and PERRY, 1963; ZOMZELY, ROBERTS and RAPAPORT, 1964; MURTHY and RAPPOPORT, 1965). It seems reasonable to assume therefore that protein synthesis in brain is controlled by a process involving messenger RNA, although there is very little direct evidence for the existence of messenger in the CNS. However several workers have demonstrated the presence of metabolically active RNA species in brain by following the incorporation of labelled precursors in v i m (BARONDES and JARVIK, 1964; ADAMS, 1965; YAMAGAMI, KAWAKITA and NAKA, 1965) and there are several reports that the synthesis of RNA is intimately involved in neural activity (CHITRE, CHOPRA and TALWAR, 1964; H Y D~N and EGYHAZI, 1964; BABICH, JACOBSON, BUBASH and JACOBSON, 1965; HYDBN and LANGE, 1965; PEVZNER, 1965). Furthermore, it has been shown that young adult rats preferentially utilize the de nouo pathway of RNA synthesis (ADAMS, 1965) and it is interesting to note that an RNA polymerase has been found in rat brain (BARONDES, 1964) and in rabbit cerebral cortex (BONDY and WAELSCH, 1964).The work described in this paper is an attempt to characterize the RNA which is synthesized in mouse brain with particular reference to the presence of messenger RNA in this tissue. Some of this work has been published as a preliminary communication in which it was stated that the level of ribosomal RNA synthesis in mouse brain is extremely low (KIMBERLIN and HUNTER, 1965). This observation is now known to be incorrect as the experiments described in the present paper show. The error resulted from the technique used to assay the radioactivity in gradient fractions of RNA which apparently behaved in a selective manner. M A T E R I A L S A N D M E T H O D S Animals. B.S.V.S. (Bacteria susceptible virus susceptible) white mice were used throughout the course of the work (SCHNEII)ER, 1959). The majority of experiments were carried out with 4-6-weekold males. Radioactive precursors (Radiochemical Centre, Amersham, Bucks), were administered intracerebrally (0.014~02 ml) using an Agla micrometer syringe (26 gauge needle) and in all cases, injections were made without the use of anaesthetics. [3H]uridine was obtained as a sterile solution in distilled water. Solutions of [3*P]orthophosphate in dilute HCl were neutralized with 0.1 M-tris before the isotope was injected. Extraction of ribosomal and soluble RNA: Coldphenol method. Mice were killed by decapitation and the brains rapidly removed. A 10 per cent brain homogenate was prepared in cold (0-4") 0.5 % NDS using a Potter-type of homogenizer. These and all subsequent stages were carried out at Mo except where otherwise stated. An equal volume of phenol reagent I (90 % phenol containing 0-1 per ~~ Abbreoiutions used: GC, Guany...

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Enzymes

Seiler¹

1969

Chemical Architecture of the Nervous System

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Cytoplasmic Protein Synthesis in Mouse Brain

Cited by 27 publications

References 28 publications

Synthesis of rapidly‐labelled brain RNA in the rat during stagnant hypoxia in the course of ontogeny

Synthesis of rapidly‐labelled brain RNA in the rat during stagnant hypoxia in the course of ontogeny

Rna Synthesis in Mouse Brain

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