Cytoplasmic polyadenylation element-binding protein 4 is highly expressed in human glioma

Abstract

Cytoplasmic polyadenylation element-binding protein 4 (CPEB4) is a highly conserved, sequence-specific RNA-binding protein that recruits translational repression or cytoplasmic polyadenylation machinery to target mRNAs. Recent studies have shown that CPEBs are expressed in somatic tissues and have essential functions supporting tumor growth, vascularization, and invasion. Overexpression of CPEB4 has been reported in pancreatic ductal adenocarcinoma and is associated with poor prognoses. However, whether CPEB4 plays a role in the tumorigenesis of gliomas is unknown. Here, we analyzed the expression of CPEB4 in gliomas. The expression profiles of CPEB4 mRNA and protein in nine normal brain tissues and 63 gliomas were detected using immunohistochemistry, real-time PCR, and western blotting. CPEB4-positive expression was significantly correlated with the pathological grade of glioma; abundant expression was observed in high-grade gliomas, whereas little or no expression was observed in normal astrocytes. Immunohistochemistry staining indicated that CPEB4 was mainly localized in the cytoplasm. In addition, CPEB4 was more highly expressed in U87 glioma cells than in U251 cells. CPEB4 expression significantly correlated with the grade in clinical gliomas. This study suggested that CPEB4 might play a role in the pathogenesis of glioma.

miRNA-130a-3p/CPEB4 Axis Modulates Glioblastoma Growth and Progression

“…Furthermore, in astrocyte tumor cells, CPEB4 knockdown inhibited their clonogenicity, proliferative ability, and invasiveness. 14 , 32 , 33 Consistent with previous studies, we also found CPEB4 was substantially increased in T98G and U87MG cell lines, and varied with different histologic grades of gliomas. What's more, single cell expression profile analysis showed CPEB4 was mainly expressed in mono/macro cells, endothelial cells, malignant cells, and oligodendrocytes, indicating that CPEB4 functioned in immune cells and stromal cells in the pathological process of gliomas.…”

“…Furthermore, in astrocyte tumor cells, CPEB4 knockdown inhibited their clonogenicity, proliferative ability, and invasiveness. 14,32,33 Consistent with previous studies, we also found CPEB4 was substantially increased in T98G and U87MG cell lines, and varied with different histologic grades of gliomas. What's more, single cell Protein-protein interaction and MCODE analysis of the target genes.…”