Cytoplasmic pH and the regulation of the dictyostelium cell cycle

Aerts, Rob J.; Durston, Antony J.; Moolenaar, Wouter H.

doi:10.1016/0092-8674(85)90237-5

Cited by 107 publications

(69 citation statements)

References 24 publications

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“…1 In addition, cytoplasmic alkalinization is an important signal in controlling the rate of cell division and activation of DNA and protein synthesis in fibroblast and Dictyostelium. 15,25 In this study, we provide several lines of evidence that eEF1A plays a direct and pivotal role in intracellular alkalinization-induced tumor cell growth. The primary function of eEF1A is to shuttle aminoacyl t-RNA during protein translation.…”

Section: Discussionmentioning

confidence: 99%

The role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth

Kim

Namkung

Yoon

et al. 2009

Laboratory Investigation

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The formation of a pH gradient, which is characterized by intracellular alkalinization and extracellular acidification, plays a key role in the growth and metastasis of tumor cells. However, the underlying mechanisms of alkalinization-induced cell growth are not known. In this study, we investigated the roles of eukaryotic translation elongation factor 1 a (eEF1A) in alkalinization-induced cell growth. In all cell lines tested (NIH3T3, HEK293, and HeLa), cell growth was affected by the modulation of intracellular pH. In general, weak intracellular alkalinization produced increased cell growth, whereas intracellular acidification resulted in decreased cell growth. It is interesting to note that portions of actin-bound eEF1A proteins were gradually reduced from acidic to alkaline conditions, suggesting an increase in levels of functionally active, free-form eEF1A. Over-expression of eEF1A caused increased cell growth in HeLa cells. It should be noted that dissociation of eEF1A from actin by transfection with the actin-binding domain deleted eEF1A construct further increased cell growth under acidic conditions, whereas most of the intact eEF1A was bound to actin. Conversely, knockdown of eEF1A by treatment with eEF1A1 and eEF1A2 siRNAs nullified the effects of alkalinization-induced cell growth. The above findings suggest that an increase in free-form eEF1A under alkaline conditions plays a critical role in alkalinization-induced cell growth.

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Section: Discussionmentioning

confidence: 99%

The role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth

Kim

Namkung

Yoon

et al. 2009

Laboratory Investigation

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“…But being the connection between entangled microscopic entities of a nonspatial nature (see the discussion in the last section of this paper), the times and ways through which the co-created information reaches the two experimenters needs not be limited by relativistic constraints, as is the case instead for macroscopic entities like an elastic band. Also, let us not forget that the most interesting aspect of the model is not its ability to perfectly simulate a specific microscopic quantum system, but to show that simple physical systems exist which are able to create correlations without communication, thus violating Bell's inequality (see also the discussion in [29]). tity, where the left fragment is, say, of length L/3 and the right fragment of length 2L/3 (see Fig.…”

Section: Discussionmentioning

confidence: 99%

Using simple elastic bands to explain quantum mechanics: a conceptual review of two of Aerts’ machine-models

Bianchi¹

2013

Open Physics

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Abstract:From the beginning of his research, the Belgian physicist Diederik Aerts has shown great creativity in inventing a number of concrete machine-models that have played an important role in the development of general mathematical and conceptual formalisms for the description of the physical reality. These models can also be used to demystify much of the strangeness in the behavior of quantum entities, by allowing to have a peek at what's going on behind the "quantum scenes," during a measurement. In this author's view, the importance of these machine-models, and of the approaches they have originated, have been so far seriously underappreciated by the physics community, despite their success in clarifying many challenges of quantum physics. To fill this gap, and encourage a greater number of researchers to take cognizance of the important work of so-called Geneva-Brussels school, we describe and analyze in this paper two of Aerts' historical machine-models, whose operations are based on simple breakable elastic bands. The first one, called the spin quantum-machine, is able to replicate the quantum probabilities associated with the spin measurement of a spin-1/2 entity.

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“…This value is taken as a measure of pHi (see below). The digitonin null-point determinations were performed as described before [15]. Monensin null-point determinations were performed in exactly the same way, except that 30 mM NaCI was added to the suspension before adding monensin (0.06%, w/v; see below).…”

Section: Phi Measurement By Ph Null-pointmentioning

confidence: 99%

Cyclic AMP induces a transient alkalinization in Dictyostelium

1987

Self Cite

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In a wide range of Cell types, stimulus-response coupling is accompanied by a rise in cytoplasmic pH (pHi). It is shown that stimulation of developing Dictyostelium discoideum cells with the chemoattractant cAMP also results in a rise in pHi. About 1.5 rain after stimulation, pHi starts increasing from pHi~7.45 to pHi ~ 7.60, as is revealed independently by two different pH null-point methods. The rise in pHi is transient, also with a persistent stimulus, and effectively inhibited by diethylstilbestrol (DES), strongly suggesting that the rise in phi is accomplished by the DES-sensitive plasma membrane proton pump which has been demonstrated in D. discoideum.

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Cytoplasmic pH and the regulation of the dictyostelium cell cycle

Cited by 107 publications

References 24 publications

The role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth

The role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth

Using simple elastic bands to explain quantum mechanics: a conceptual review of two of Aerts’ machine-models

Cyclic AMP induces a transient alkalinization in Dictyostelium

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