Cytoplasmic modifications at the contact zone of osteoclasts and calcified tissue in the diaphyseal growing plate of foetal guinea-pig tibia

Malkani, K; Luxembourger, M. M.; Rèbel, A

doi:10.1007/bf02547225

Cited by 40 publications

(23 citation statements)

References 7 publications

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“…Although the clear zone may change into a ruffled border (MALKANI et al, 1973), the present studies suggest the movement of osteoclasts from a resorbing lacuna to an adjacent one while the clear zone remains visible : this would explain why the ring-shaped structures were always observed in positions corresponding to the the contours of the lacune. However, the structural phase of the clear zone corresponding to the stage of resorption could not be determined.…”

Section: Discussionmentioning

confidence: 43%

“…The clear zone has generally been reported as an electron dense and organella-free area (SCHENK et al, 1967;MALKANI et al, 1973;HOLTROP et al, 1974;KING and HOLTROP, 1975;SAKURAOKA, 1984). It was further divided into a dark and light area (MALKANI et al, 1973), and in the dark area, 5-7 nm actin Fig, 6), the clear zone shifted out from the lacuna (Type 2, Fig.…”

Section: Discussionmentioning

confidence: 97%

“…This has three-dimensionally observed, and the cytoplasmic processes forming the ruffled border have been classified into two types WAKITA, 1986, 1989). On the lateral side of the ruffled border, there is a clear zone which appears as an electron dense area free of cell organelles under the transmission electron microscope (SCHENK et al, 1967;MALKANI et al, 1973;HOLTROP et al, 1974;KING and HOLTROP, 1975;SAKURAOKA, 1984). It has been shown that the clear zone completely encircles the ruffled border (HOLTROP et al, 1974).…”

Section: Discussionmentioning

confidence: 98%

“…KING and HoLTROP (1975) reported that the clear zone seals the territory of the ruffled border from the outside. The clear zone has a striated appearance with alternating dark and light areas oriented perpendicular to the bone surface (MALKANI et al, 1973), and binding to heavy meromyosin (KING and HOLTROP, 1975). ZALLONE et al (1982ZALLONE et al ( , 1983) demonstrated the clear zone of the cultured osteoclasts on glass cover slips by using an indirect immunofluorescent antibody method and purified actin antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…It was further divided into a dark and light area (MALKANI et al, 1973), and in the dark area, 5-7 nm actin Fig, 6), the clear zone shifted out from the lacuna (Type 2, Fig. 7), and the clear zone where no lacuna has been formed (Type 3, Fig.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Studies of the clear zone of osteoclasts: Immunohistological aspects of its form and distribution.

Yoshida

Domon

Wakita

1989

Archives of Histology and Cytology

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Section: Discussionmentioning

confidence: 43%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Studies of the clear zone of osteoclasts: Immunohistological aspects of its form and distribution.

Yoshida

Domon

Wakita

1989

Archives of Histology and Cytology

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Cytoskeletal changes in osteoclasts during the resorption cycle

Lakkakorpi

Väänänen

1996

Microsc. Res. Tech.

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Ultrastructural features of odontoclasts that resorb enamel in human deciduous teeth prior to shedding

et al. 1998

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Three dental hard tissues, i.e., cementum, dentin, and enamel, are resorbed by multinucleated cells referred to as "odontoclasts." These cells have morphological and functional characteristics similar to those of bone-resorbing osteoclasts. However, concerning enamel resorption, which is a process that may occur during tooth eruption, satisfactory ultrastructural data on odontoclastic resorption are still lacking. Ultrastructural and histochemical characteristics of odontoclasts resorbing enamel of human deciduous teeth prior to shedding were examined by means of light microscopy and transmission and scanning electron microscopy. Odontoclasts that that resorbed enamel were tartrate-resistant acid phosphatase (TRAP)-positive multinucleated giant cells that were essentially the same as those that resorbed dentin and cementum. Ultrastructurally, they had numerous mitochondria, lysosomes, and free polysomes in their cytoplasm. In addition, they were characteristically rich in large cytoplasmic vacuoles containing enamel crystals in the cytoplasm opposite the ruffled border. Although they extended a well-developed, ruffled border against enamel surface, a clear zone--an area typically devoid of organelles--was rarely seen in these cells. In many cases, the cells were in very close contact with the enamel surface by the peripheral part of their cytoplasm. The enamel prisms at the resorption surface contained more loosely packed and electron-lucent enamel crystals compared with those of unresorbed, intact enamel. Furthermore, numerous thin needle- or plate-like enamel crystals that were liberated from the enamel matrix were found in the extracellular channels of the ruffled border and in various-sized cytoplasmic vacuoles in their cytoplasm. The superficial layer of the enamel matrix undergoing odontoclastic resorption stained positively with toluidine blue and for TRAP activity. The results of the present study suggest that odontoclasts resorbing enamel secrete acids as well as organic components, including hydrolytic enzymes, into the resorption zone underlying their ruffled border and that they phagocytose crystals that have been liberated from the partially demineralized enamel matrix by acids, subsequently dissolving them intracellularly.

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Cytoplasmic modifications at the contact zone of osteoclasts and calcified tissue in the diaphyseal growing plate of foetal guinea-pig tibia

Cited by 40 publications

References 7 publications

Studies of the clear zone of osteoclasts: Immunohistological aspects of its form and distribution.

Studies of the clear zone of osteoclasts: Immunohistological aspects of its form and distribution.

Cytoskeletal changes in osteoclasts during the resorption cycle

Ultrastructural features of odontoclasts that resorb enamel in human deciduous teeth prior to shedding

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