Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence

Ferreira, E.; Vireque, A.A.; Adona, Paulo Roberto; Meirelles, Flávio Vieira; Ferriani, Rui Alberto; Navarro, Paula Andrea

doi:10.1016/j.theriogenology.2008.10.023

Cited by 267 publications

(244 citation statements)

References 85 publications

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“…During oocyte maturation, there are marked changes in the distribution of mitochondria that supply the majority of the cellular ATP (Yu, Dumollard, Rossbach, Lai, & Swann, 2010). The difference in mitochondria localization has been associated with developmental competence of oocytes (Ferreira et al., 2009). The mitochondrial membrane potential must be maintained to support ATP synthesis; however, a high potential will favor the production of deleterious ROS (Merry, 2002).…”

Section: Discussionmentioning

confidence: 99%

SIRT4 is essential for metabolic control and meiotic structure during mouse oocyte maturation

Zeng

Jiang²,

et al. 2018

Aging Cell

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SIRT4 modulates energy homeostasis in multiple cell types and tissues. However, its role in meiotic oocytes remains unknown. Here, we report that mouse oocytes overexpressing SIRT4 are unable to completely progress through meiosis, showing the inadequate mitochondrial redistribution, lowered ATP content, elevated reactive oxygen species (ROS) level, with the severely disrupted spindle/chromosome organization. Moreover, we find that phosphorylation of Ser293‐PDHE1α mediates the effects of SIRT4 overexpression on metabolic activity and meiotic events in oocytes by performing functional rescue experiments. By chance, we discover the SIRT4 upregulation in oocytes from aged mice; and importantly, the maternal age‐associated deficient phenotypes in oocytes can be partly rescued through the knockdown of SIRT4. These findings reveal the critical role for SIRT4 in the control of energy metabolism and meiotic apparatus during oocyte maturation and indicate that SIRT4 is an essential factor determining oocyte quality.

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Section: Discussionmentioning

confidence: 99%

SIRT4 is essential for metabolic control and meiotic structure during mouse oocyte maturation

Zeng

Jiang²,

et al. 2018

Aging Cell

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“…Oocytes are attached to and are surrounded by granulosa cells, which form the cumulus-oocyte complex (COC), and by theca cells (Morgan et al, 2015;Zhang & Liu, 2015). The maturation process of the oocyte includes both the nuclear (meiosis) and cytoplasmic maturation processes (reorganization of organelles and cytoskeleton, transcription of RNAs, and translation of proteins related to embryonic development) (Ferreira et al, 2009). The in vitro viability of COCs is essential for Downloaded from https://www.cambridge.org/core.…”

Section: Introductionmentioning

confidence: 99%

Effects of PI3K and FSH on steroidogenesis, viability and embryo development of the cumulus–oocyte complex after in vitro culture

Souza

Salles

Camargo

et al. 2017

Zygote

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SummaryThe purpose of this study was to evaluate the effects of FSH and PI3K on the nuclear maturation, viability, steroidogenesis and embryo development of bovine cumulus-oocyte complexes (COCs). Oocyte maturation was achieved with MIV B, MIV B+100 µM LY294002, MIV B+10 ng/mL follicle stimulating hormone (FSH), or MIV B+10 ng/mL FSH+100 µM LY294002 treatments for 22-24 h. After the cultured COCs were denuded, oocytes were separated into those that extruded polar bodies (mature) and those that did not, and real-time polymerase chain reaction (PCR) for BAX, BCL2, LHR, FSHR, CYP11A1, CYP19A1 and HSD17B1 genes was performed. The culture medium was collected to determine the levels of 17β-estradiol (E2) and progesterone (P4). The trypan blue test was used to study COC viability, and embryo development was evaluated. FSH increased nuclear maturation and PI3K blocked the maturation but did not influence oocyte viability. BAX and BCL2 expression levels in the cumulus cells were only affected by FSH, and the BAX levels decreased after treatment with LY294002. FSH increased the levels of E2 and P4, however inhibition of PI3K decreased E2 levels. MIV B enhanced levels of LHR, FSHR, CYP11A1, CYP19A1 and HSD17B1, whereas LY294002 inhibited the expression levels of all genes. MIV B+FSH decreased the expression levels of all genes except CYP11A1. LY294002 did not demonstrate any effects in the presence of FSH. Embryo development was significantly decreased when the MIV B+FSH medium was used. In conclusion, FSH controls the steroidogenesis, viability and gene expression in COCs. PI3K plays essential roles in nuclear maturation, steroidogenesis and embryo development.

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“…Ultrastructural re-organization involves the redistribution of microfilaments and microtubules that in turn support the relocation of cytoplasmic organelles such as cortical granules and mitochondria [Schorderet-Slatkine 1972;Masui 1985;Iwamatsu et al 1993;Voronina and Wessel 2003;Sun and Schatten 2006;Stricker 2006;Ferreira et al 2009;Liu et al 2010; De los Reyes et al 2011].…”

Section: Introductionmentioning

confidence: 99%

Ion currents modulating oocyte maturation in animals

Tosti

Boni

Gallo

et al. 2013

Systems Biology in Reproductive Medicine

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Growing oocytes are arrested at the first prophase of meiosis which is morphologically identified by the presence of a large and vesicular nucleus, called the germinal vesicle. The dissolution of the germinal vesicle marks the resumption of meiosis during which the oocyte undergoes massive modifications up to the second meiotic block, which is removed at fertilization. The interval between the first and the second meiotic block is defined as maturation and the events occurring during this period are crucial for ovulation, fertilization, and embryo development. Oocytes are excitable cells that react to stimuli by modifying their electrical properties as a consequence of ion currents flowing through ion channels on the plasma membrane. These electrical changes have been largely described at fertilization whereas little information is available during oocyte maturation. The aim of this review is to give an overview on the involvement of ion channels and ion currents during oocyte maturation in species from invertebrates to mammals. The results summarized here point to the possible functional role of ion channels underlying oocyte growth and maturation.

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Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence

Cited by 267 publications

References 85 publications

SIRT4 is essential for metabolic control and meiotic structure during mouse oocyte maturation

SIRT4 is essential for metabolic control and meiotic structure during mouse oocyte maturation

Effects of PI3K and FSH on steroidogenesis, viability and embryo development of the cumulus–oocyte complex after in vitro culture

Ion currents modulating oocyte maturation in animals

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