Cytoplasmic glutathione regulated by cumulus cells during porcine oocyte maturation affects fertilization and embryonic development in vitro

Maedomari, Naoki; Kikuchi, Kazuhiro; Ozawa, Manabu; Noguchi, Junko; Kaneko, Hiroyuki; Ohnuma, Katsuhiko; Nakai, Michiko; Shino, Masao; Nagai, Takashi; Kashiwazaki, Naomi

doi:10.1016/j.theriogenology.2006.11.012

Cited by 99 publications

(92 citation statements)

References 49 publications

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“…Related to this, it has been suggested that the GSH may play an important role in fertilization but also in embryonic development (Maedomari et al 2007). It is speculated that the GSH may exert a promoting effect on MPN formation by acting synergistically in the following ways: GSH promotes the disruption of the disulfide bond of the protamine in the sperm chromatin by shifting the oocyte cytoplasm into a redox state; and/or the GSH acts as a substrate of glutathione peroxidase and as a scavenger of free radicals in oocytes, enhancing their competence as a whole (Nagai 1996).…”

Section: Discussionmentioning

confidence: 97%

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

García-Mengual¹,

Silvestre²,

Salvador³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:In pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitro culture (IVC) medium post-ICSI was supplemented with 1.71mM cysteine (CYS). Alternatively, the sperm membrane was digested with Triton X-100 (TX) before ICSI, to improve the exposure of the sperm chromatin to the oocyte cytoplasm. After 6 h post-ICSI, the activation rate was significantly higher in TX group (70.0%) compared with CYS and control groups (42.2% and 48.9%, respectively; P < 0.05). However, no significant differences between the three groups were observed in terms of the number of pronuclei, 2PN (oocytes with 2 pronuclei and no visible sperm), and 1PN + sperm (oocytes with 1 pronucleus and one sperm head). At 22 h post-ICSI, the activation rates were similar in TX, CYS, and control groups (73.1, 78.9, and 75.7%, respectively). In addition, we did not observe significant differences between TX, CYS, and control groups for the number of pronuclei, 2PN (52.6, 56.7, and 50%, respectively) or 1PN + sperm (21.1, 33.3, and 32.1%, respectively). While no cleavage was observed in the CYS group, no significant differences in the cleavage rate were observed between control (21.3%) and TX (10.5%) groups. In summary, and under our conditions, neither CYS supplement, nor sperm TX pre-treatment were able to improve MPN formation at 6 and 22 h post-ICSI. However, the sperm TX pre-treatment improved oocyte activation at 6 h post-ICSI, although 22 h post-ICSI such a beneficial effect did not persist.Keywords: porcine; assisted reproductive technology; pronuclear formation List of abbreviations: ICSI = intracytoplasmic sperm injection, PN = pronucleus, MPN = male pronucleus, IVC = in vitro culture, CYS = cysteine, TX = Triton X-100, IVP = in vitro production, IVM = in vitro maturation, IVF = in vitro fertilization, EGF = epidermal growth factor, GSH = glutathione, COC = cumulus oocyte complex, PZM-3 = porcine zygote medium-3, MM199 = maturation medium, HM199 = handling medium 242

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Section: Discussionmentioning

confidence: 97%

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

García-Mengual¹,

Silvestre²,

Salvador³

et al. 2015

CZECH J ANIM SCI

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“…In this respect, the composition of IVM medium to support recovery processes may be important. Also it is highly plausible that presence of cumulus cells and maintaining cumulus functions (e.g., gap junctional communications between cumulus cells and oocyte) is indispensable for this process because cumulus cells and the junctions have basic importance for porcine oocytes to acquire developmental competence and normal mitochondrial functions during maturation [68,69]. In addition, the timing of vitrification during the GV stage seems to have a huge impact on porcine oocytes to regain their meiotic competence.…”

Section: Immature Oocytes; Low Survival But the Developmental Abilitymentioning

confidence: 99%

Factors Affecting Cryopreservation of Porcine Oocytes

Somfai

Kikuchi

Nagai

2012

Journal of Reproduction and Development

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“…Lebih lanjut Kim et al, (1999) juga melaporkan penambahan 1 mM GSH dalam media fertilisasi, jumlah embrio sapi yang mencapai tahap blastosis lebih tinggi (27,3%) dibandingkan dengan 0 mM (20,1%), 0,1 mM (21,8%) dan 10 mM (8,9%). Selanjutnya Maedomari et al, (2007) menyatakan GSH yang terdapat pada sitoplasma oosit berperan dalam proses pembentukan pronukleus dimulai dari pemecahan ikatan disulfide membran inti yang dilanjutkan dengan inisiasi dekondensasi kromosom. Lebih lanjut Zuelke et al, (2003) menjelaskan pada oosit yang matang, GSH mempunyai peran penting dalam pembentukan pronukleus jantan setelah fertilisasi.…”

Section: Glutathioneunclassified

“…Konsentrasi GSH dalam sitoplasma oosit hasil pematangan in vitro tergantung pada kondisi awal proses pematangan yang ditentukan oleh keberadaan sel-sel komulus. Konsentrasi GSH dalam oosit menunjukkan tingkat kematangannya dan GSH memengaruhi keberhasilan dan perkembangan embrio selanjutnya (Maedomari et al, 2007). Lebih lanjut dijelaskan di bawah kondisi fisiologi, level GSH tinggi pada oosit tikus dan hamster, penting untuk pembentukan pronukleus jantan setelah fertilisasi dan mendukung perkembangan embrio dini (Zuelke et al, 2003).…”

Section: Glutathioneunclassified

Fertilitas Dan Persentase Embrio Kerbau Sampai Morula Yang Dikultur Dengan Penambahan Glutathione Secara in Vitro

Harissatria

Hendri

2016

jupet

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Cytoplasmic glutathione regulated by cumulus cells during porcine oocyte maturation affects fertilization and embryonic development in vitro

Cited by 99 publications

References 49 publications

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

Factors Affecting Cryopreservation of Porcine Oocytes

Fertilitas Dan Persentase Embrio Kerbau Sampai Morula Yang Dikultur Dengan Penambahan Glutathione Secara in Vitro

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