Cytophysiological features of<i>Posidonia oceanica</i>as putative markers of environmental conditions

Cozza, Radiana; Chiappetta, Adriana; Petrarulo, M.; Salimonti, Amelia; Rende, Sante Francesco; Bitonti, Maria Beatrice; Innocenti, A. M.

doi:10.1080/02757540410001689777

Cited by 17 publications

(19 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Posidonia oceanica, PoCHL P was expressed primarily in the leaves and transcripts accumulated mainly in the epidermal layer. This observation is consistent with the anatomy of Posidonia leaves in which the epidermal cells harbor numerous chloroplasts in contrast to the mesophyll cells; the latter contain few chloroplasts and exhibit large vacuoles that are often entirely filled with phenolics (Mariani Colombo et al 1983, Cozza et al 2004). Notably, PoCHL P transcripts were also detected in the leaf phloem tissue -a finding that has not been previously reported (Giannino et al 2004, Bruno et al 2009a.…”

Section: Discussionsupporting

confidence: 84%

“…However, the pattern of gene expression did not overlap between plants growing in different meadows that were previously categorized as preserved (PM) and disturbed (DM) sites for Posidonia oceanica based on several relevant fitness parameters (Cozza et al 2004, Rende et al 2005, Acunto et al 2006, Bruno et al 2009b. Indeed, in plants from preserved sites, PoCHL P expression was higher in fast-growing YL and IL than in AL -an observation that is consistent with the pattern described for pluriannual vs. deciduous leaves of terrestrial plants (Bruno et al 2009a).…”

Section: Discussionmentioning

confidence: 97%

“…Given the subsidiary role of CHL P in both chl and TP biosynthesis, the similar levels of chl in AL of PM and DM plants prompt us to speculate that the high level of PoCHL P expression in these leaves may be related to TP requirement as an adaptive response to shield plants from both oxidative and aging processes (Weaver & Amasino 2001, Havaux et al 2005. Oxidative and aging processes could precociously occur in AL of PM plants due to the absence of adequate levels of cytokinins (Cozza et al 2004), which are known to play a key role in preventing leaf senescence. Moreover, TP might be required to sustain resource translocation (Hofius et al 2004) at a critical moment in the plant life cycle.…”

Section: Discussionmentioning

confidence: 99%

“…These meadows were previously classified as preserved and disturbed based on crucial ecological descriptors (Rende et al 2005, Acunto et al 2006, hormonal parameters (Cozza et al 2004, Bruno et al 2009b) and proteomic data (Mazzuca et al 2009). Because the disturbed site was characterized by higher water turbidity (Rende et al 2005, Acunto et al 2006), the present study monitored light conditions in the 2 meadows over a 1 yr period.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

Bruno¹,

Bruno²,

Chiappetta³

et al. 2010

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

In past decades, meadows of Posidonia oceanica (L) Delile have declined extensively in the Mediterranean basin mainly due to disturbances associated with reduced light availability. To investigate the molecular mechanisms of photoacclimation in this seagrass, we selected the CHL P gene as a marker of plant response to light availability because it is involved in the chlorophylltocopherols biosynthetic pathways. After characterizing the PoCHL P gene, its expression pattern was analyzed in leaves of plants growing in 2 distinct meadows that were previously categorized as disturbed and preserved. Plants were sampled during different seasons and light availability was monitored in the selected meadows over a 1 yr period. PoCHL P expression was also analyzed in leaves of plants that were exposed to controlled light conditions in aquaria. We found that PoCHL P was expressed differentially with respect to leaf developmental stage and that its expression pattern changed rapidly under light conditions far below the compensation value established for P. oceanica. Hence, the recognition of this gene as a reasonable marker of critical light conditions provides an important step towards the functional characterization of stress-related genes in seagrasses. KEY WORDS: PoCHL P expression · Posidonia oceanica · Light availabilityResale or republication not permitted without written consent of the publisher

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

Bruno¹,

Bruno²,

Chiappetta³

et al. 2010

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…The resulting insoluble precipitate compromises subsequent protein purification irreversibly. Since P. oceanica leaves contain high levels of simple and complex polyphenolic compounds (Agostini et al 1998;Ferrat et al 2003;Cozza et al 2004;Dumay et al 2004) and low levels of proteins, the main aim of this study was the removal of non-protein compounds before protein precipitation and purification.…”

Section: Introductionmentioning

confidence: 99%

2-DE polypeptide mapping ofPosidonia oceanicaleaves, a molecular tool for marine environment studies

Spadafora

Filadoro

Mazzuca

et al. 2008

Plant Biosystems - An International Journal Dealing with all As

View full text Add to dashboard Cite

The aim of this research is to provide a molecular tool based on polypeptide mapping to investigate the flowering marine plant Posidonia oceanica. This plant is very vulnerable to contaminants; thus it is considered a valuable bio-indicator of water quality in bio-monitoring of coastal environments. Posidonia oceanica was found to be recalcitrant to the common protein extraction methods. In the present work, three different extraction procedures were compared to obtain high yield and quality protein extracts suitable for mono-dimensional and bi-dimensional electrophoresis (1-DE and 2-DE). Proteins were extracted from juvenile, intermediate and adult leaves in order to assess the influence of tissue differentiation on protein yield. The highest protein yield was obtained with 20% trichloroacetic acid (TCA) precipitation of proteins. The best extraction efficiency was found in juvenile leaves as compared with intermediate and adult ones. However, as a large amount of juvenile leaves is required for obtaining sufficiently large protein samples, these were considered not suitable for the electrophoretic analysis. Extensive sampling could introduce further damage in the meadows under study. High quality 2-DE polypeptide mappings were obtained only from intermediate and adult leaves; the good reproducibility of protein patterns indicates that this approach could be used to explore changes in protein expression of P. oceanica in response to altered environmental conditions.

show abstract

Differential Expression of Proteins in Red Pear Following Fruit Bagging Treatment

et al. 2011

View full text Add to dashboard Cite

Fruit bagging is a very effective method for study of fruit qualities and anthocyanin synthesis. The characterization of differentially expressed proteins that were isolated from both bagged and normal fruit skin tissue is apparently an essential parameter for understanding the effect of shading on fruit qualities and to understand the mechanism of fruit coloring in Pyrus communis. Proteome maps of both bagged and normal P. communis 'Placer' fruit skin were obtained by performing two-dimensional electrophoresis analysis and compared to assess the extent to which protein distribution differed in pear skin. The comparative analysis showed 38 differentially expressed proteins between the two samples: with three protein spots up-regulated and 35 down-regulated in the bagged fruit. Differentially expressed protein spots were subjected to matrix-assisted laser desorption ionization time of flight (MALDI-TOF) analysis and the data compared to that of known proteins to deduce their possible functions. Of these, 21 protein spots were identified and classified into functional classes. These identified proteins were mainly involved in photosynthesis, signal transduction, energy pathway, protein folding and assembly, and carbohydrate and acidity metabolisms, and were under-expressed in bagged fruit skins. This work provides a first characterization of the proteome changes in response to fruit bagging treatment in red pears.

show abstract

Cytophysiological features ofPosidonia oceanicaas putative markers of environmental conditions

Cited by 17 publications

References 41 publications

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

2-DE polypeptide mapping ofPosidonia oceanicaleaves, a molecular tool for marine environment studies

Differential Expression of Proteins in Red Pear Following Fruit Bagging Treatment

Contact Info

Product

Resources

About