Cytophotometric and biochemical characterization of<i>Posidonia oceanica</i>L. (Potamogetonaceae) genome

Cavallini, Andrea; Natali, Lucia; Giordani, Tommaso; Polizzi, Enza; Balestri, Elena; Cinelli, Francesco; Maserti, Biancaelena; Ferrara, R.

doi:10.1080/00087114.1995.10797330

Cited by 9 publications

(4 citation statements)

References 23 publications

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“…The 2C-genome size of Posidonia was established in 5–6 pg DNA (Cavallini et al, 1995; Koce et al, 2003), posing this species in the middle range of monocots, being genome much larger than in other seagrasses as Cymodocea nodosa (1.1 pg), Zostera nolti (1.5 pg), and Z. marina (1.2 pg) (Koce et al, 2003). Such differences are largely independent on chromosome number and hence on polyploidization: P. oceanica has 2 n = 20 and Zostera species have 2 n = 12 (Koce et al, 2003).…”

Section: Molecular Studies On Posidonia Oceanica Are Still Limitedmentioning

confidence: 99%

“…Such differences are largely independent on chromosome number and hence on polyploidization: P. oceanica has 2 n = 20 and Zostera species have 2 n = 12 (Koce et al, 2003). P. oceanica genome has been characterized biochemically (Cavallini et al, 1995; Maestrini et al, 2002). At molecular level, only a few repetitive DNA sequences are found in the NCBI nucleotide database.…”

Section: Molecular Studies On Posidonia Oceanica Are Still Limitedmentioning

confidence: 99%

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Potential applications of next generation sequencing to the genomics of Posidonia oceanica

Cavallini¹,

Natali²,

Giordani³

2012

Front. Plant Sci.

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Section: Molecular Studies On Posidonia Oceanica Are Still Limitedmentioning

confidence: 99%

Section: Molecular Studies On Posidonia Oceanica Are Still Limitedmentioning

confidence: 99%

Potential applications of next generation sequencing to the genomics of Posidonia oceanica

Cavallini¹,

Natali²,

Giordani³

2012

Front. Plant Sci.

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“…The aim of this work was evaluating the potential activation of retrotransposition events in P. oceanica by measuring the transcription of LTR-RE sequences, which represents the first step of the retrotransposition process. Unfortunately, because of the large size of P. oceanica genome (more than 5 Gbp [37]), which would require very high sequencing coverage to obtain large sequenced scaffolds, no full-length LTR-REs are available of this species. For this reason, we conducted a meta-analysis using a library of LTR-RE fragments previously identified in P. oceanica [38].…”

Section: Introductionmentioning

confidence: 99%

Low Long Terminal Repeat (LTR)-Retrotransposon Expression in Leaves of the Marine Phanerogam Posidonia Oceanica L.

Vangelisti

Mascagni

Usai

et al. 2020

Life

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Seagrasses as Posidonia oceanica reproduce mostly by vegetative propagation, which can reduce genetic variability within populations. Since, in clonally propagated species, insurgence of genetic variability can be determined by the activity of transposable elements, we have estimated the activity of such repeat elements by measuring their expression level in the leaves of plants from a Mediterranean site, for which Illumina complementary DNA (cDNA) sequence reads (produced from RNAs isolated by leaves of plants from deep and shallow meadows) were publicly available. Firstly, we produced a collection of retrotransposon-related sequences and then mapped Illumina cDNA reads onto these sequences. With this approach, it was evident that Posidonia retrotransposons are, in general, barely expressed; only nine elements resulted transcribed at levels comparable with those of reference genes encoding tubulins and actins. Differences in transcript abundance were observed according to the superfamily and the lineage to which the retrotransposons belonged. Only small differences were observed between retrotransposon expression levels in leaves of shallow and deep Posidonia meadow stands, whereas one TAR/Tork element resulted differentially expressed in deep plants exposed to heat. It can be concluded that, in P. oceanica, the contribution of retrotransposon activity to genetic variability is reduced, although the nine specific active elements could actually produce new structural variations.

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“…Some attention has been paid to seagrasses (Cavallini et al 1995;Dolenc Koce et al 2003) and halophytes (Dolenc Koce 2008). Concerning freshwaterrelated plants, some specific taxonomic groups partly, mostly or entirely formed by these kind of organisms have been investigated.…”

mentioning

confidence: 99%

Genome size in aquatic and wetland plants: fitting with the large genome constraint hypothesis with a few relevant exceptions

et al. 2015

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Cytophotometric and biochemical characterization ofPosidonia oceanicaL. (Potamogetonaceae) genome

Cited by 9 publications

References 23 publications

Potential applications of next generation sequencing to the genomics of Posidonia oceanica

Potential applications of next generation sequencing to the genomics of Posidonia oceanica

Low Long Terminal Repeat (LTR)-Retrotransposon Expression in Leaves of the Marine Phanerogam Posidonia Oceanica L.

Genome size in aquatic and wetland plants: fitting with the large genome constraint hypothesis with a few relevant exceptions

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