Cytophotometric analysis of cervical intraepithelial neoplasia grade III, with and without synchronous invasive squamous cell carcinoma

Abstract

Cytophotometric analysis was performed in nuclei retrieved from paraffinembedded cervical tissue from 57 cases of CIN 111. CIN 111 lesions of patients without invasive squamous cell carcinoma (N = 37) were regarded to represent a mixture of progressive and nonprogressive lesions. The CIN 111 lesions of patients with a synchronous invasive squamous cell carcinoma (N = 20) were regarded as representing truly progressive precursor lesions (C1N.INV). Twenty-one photometric features describing geometrical, density, and texture characteristics were extracted from the digitized nuclear images. Statistical analysis of cytophotometric data indicated significant differences between the group of CIN 111 lesions and CIN.INV lesions. A cluster analysis, using one co-occurrency texture feature (S-HOMOG), one density feature (S-DI), and two geometrical features (S-AREA and M-CIRC), showed that two clusters (Cl and C2) were present in the total group of CIN 111 and CIN.INV lesions. The vast majority of CIN.INV lesions was member of one and the same cluster C1. The CIN I11 group appeared to consist of a mixture of two clusters, 54% C1 and 46% C2 lesions. Of patients 45 years or younger, the majority (62%) of CIN 111 lesions had feature values, corresponding with those of cluster Cl, and as such possibly with a potentially progressive course. In patients older than 45 years the percentage of CIN 111 lesions with C1 feature values was 27%.

DNA cytometry confirms the utility of the Bethesda system for the classification of Papanicolaou smears

“…The aneuploid LSIL probably belongs to the group that has the ability to progress to biopsy proven CINIII/CIS. 7 It has been shown that in up to 30% of cases of LSIL, a follow-up biopsy will show CINIII/CIS. 15,4 The predictive positive value of DNA aneuploidy to predict those cases of the diagnostic Group IIID of the Munich II classification that will progress to CINIII/CIS was only 42%.…”

“…[7][8][9]12,16,17,[23][24][25]27, DNA aneuploidy has been regarded as an objective marker for progressive CIN. 7,33,60 DNA cytometry can be done on a routine basis using PC-based commercially available cytometers, which makes the diagnosis of DNA ploidy easy. 25 Research indicates that HSIL lesions are probably induced by oncogenic human papilloma viruses (HPVs).…”

“…6 Nowadays, DNA aneuploidy detected by image cytometry of Feulgen-stained Pap smears indicates a potentially progressive lesion, that is, a high-grade lesion. 7 Our own results have shown that DNA cytometry can identify those cases of dysplasia (Munich Group IIID) that are likely to progress to CINIII/CIS. 8,9 In this study, we compared the validity of interactive DNA cytometry with Pap smears classified according to the Bethesda System.…”

Morphoquantitative Analysis of the Intraepithelial Neoplasic Uterine Cells by Computer Image System

“…In fact, they made us disqualify several subjects' material (61.1%) for having a totally irregular distribution of chromogen results in diverse nucleus cells and a major intensity in a specific place, perhaps, due to the different fixing agents during impregnation method (Hanselaar et al, 1992). The usage of different fixing agents with different fixation methods shows some varieties about chromogen intensity in the ionic hydrogen concentration and the hydrolise time in Feulgen's reaction (Böhm;Böhm et al, 1968) that could interfere in the condensation of chromatin's spatial distribution and, apart from tracking the DNA, does the same with proteins (Stedman & Stedman, 1950), Umayahara et al (2002).…”

Utility of Residual AutoCyte Cervical Cytology Samples for Image Analysis

“…9 A particularly useful ancillary study in cervical cytology would be DNA profiling, as performed earlier. 13,18,20,26 The process of destaining these conventional smears and restaining with the Feulgenthionin procedure, however, leads to unpredictable losses in stainable content according to some investigators. 1 Other investigators have resorted to disaggregation of nuclei from subsequent cervical biopsies in order to investigate DNA ploidy of cervical lesions.…”