Cytometric determination of novel organotellurium compound toxicity in a promyelocytic (HL-60) cell line

Sailer, Brian L.; Liles, Nathan; Dickerson, Sarah; Chasteen, Thomas G.

doi:10.1007/s00204-002-0407-x

Cited by 37 publications

(23 citation statements)

References 38 publications

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“…Moreover, it was described that the compound AS101 may be involved in G2/ M growth arrest and induced apoptosis in multiple myeloma [30]. Consistently, DPDT treatment induced a time-dependent increase in the number of apoptotic cells from the S and G2/ M portions of the cell cycle in HL-60 cells [3]. This suggests that DPDT interferes either with DNA replication or with cell division processes.…”

Section: Discussionmentioning

confidence: 73%

“…For this, we applied the enzymatic morphological analysis that showed dose-dependent induction of apoptosis and necrosis at all time periods studied (table 2). Sailer et al [3] demonstrated a cell cycle-specific (from S and G2 phase) apoptosis induction in HL-60 cells after DPDT exposure. As caspases are important mediators of apoptosis [22], we determined apoptosis in DPDT-treated cells by the luminescence-based caspase 3/7 assay.…”

Section: Discussionmentioning

confidence: 99%

“…However, the underlying mechanism of toxicity of tellurium (Te) compounds leading to cell death has been poorly investigated. Some studies have indicated that OT compounds can induce apoptosis and cell cycle arrest in cancer cells such as human promyelocytic (HL-60) cell line [3]. In this context, data from our laboratory showed that DPDT-induced DNA double-strand breaks (DSBs) in permanent lung fibroblast cell line derived from Chinese hamster (V79 cells).…”

mentioning

confidence: 96%

“…In the last few decades, evidence has been accumulating that OT molecules are promising pharmacological agents. Several reports have been published showing immuno-modulatory, antioxidant, anticancer and anti-inflammatory properties of OT compounds [3][4][5]. Despite the growing use of OT compounds in the chemical and biochemical fields, there has been little concern about their toxicity.…”

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confidence: 99%

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Diphenyl Ditelluride‐Induced Cell Cycle Arrest and Apoptosis: A Relation with Topoisomerase I Inhibition

Jorge

Oliveira

Filippi-Chiela

et al. 2014

Basic Clin Pharma Tox

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The diphenyl ditelluride (DPDT) is a prototype for the development of new biologically active molecules. In previous studies, DPDT showed an elevated cytotoxicity in Chinese hamster fibroblast (V79) cells but the mechanisms for reduction of cell viability still remain unknown. DPDT showed mutagenic properties by induction of frameshift mutations in bacterium Salmonella typhimurium and yeast Saccharomyces cerevisiae. This organotelluride also induced DNA strand breaks in V79 cells. In this work, we investigated the mechanism of DPDT cytotoxicity by evaluating the effects of this compound on cell cycle progression, apoptosis induction and topoisomerase I inhibition. Significant decrease of V79 cell viability after DPDT treatment was revealed by MTT assay. Morphological analysis showed induction of apoptosis and necrosis by DPDT in V79 cells. An increase of caspase 3/7 activity confirmed apoptosis induction. The cell cycle analysis showed an increase in the percentage of V79 cells in S phase and sub-G1 phase. The yeast strain deficient in topoisomerase I (Topo I) showed higher tolerance to DPDT compared with the isogenic wild-type strain, suggesting that the interaction with this enzyme could be involved in DPDT toxicity. The sensitivity to DPDT found in top3Δ strain indicates that yeast topoisomerase 3 (Top3p) could participate in the repair of DNA lesions induced by the DPDT. We also demonstrated that DPDT inhibits human DNA topoisomerase I (Topo I) activity by DNA relaxation assay. Therefore, our results suggest that the DPDT-induced cell cycle arrest and reduction in cell viability could be attributed to interaction with topoisomerase I enzyme.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

mentioning

confidence: 99%

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Diphenyl Ditelluride‐Induced Cell Cycle Arrest and Apoptosis: A Relation with Topoisomerase I Inhibition

Jorge

Oliveira

Filippi-Chiela

et al. 2014

Basic Clin Pharma Tox

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“…15 However, to the best of our knowledge, the cytotoxicity profile of tellurium nanostructures has yet to be established.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Potentials of Tellurium Nanowires in BALB/3T3 Fibroblast Cells

Mahto¹,

Kim²,

Rhee³

2011

Bulletin of the Korean Chemical Society

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We have investigated the cytotoxic potential of tellurium (Te) nanowires in BALB/3T3 fibroblast cells. Te nanowires were synthesized through an aqueous phase surfactant assisted method. Toxicological experiments, such as analysis of morphological changes, MTT assay, DAPI staining, and estimation of intracellular reactive oxygen species, were carried out to reveal the cytotoxic effects of Te nanowires. Te nanowires were found to be cytotoxic at all concentrations tested, in a dose-dependent manner. The UV/Vis spectra of Te nanowires suspended in a culture medium showed drastic changes and disappearance of two broad absorption peaks. The physicochemical properties such as, surface charge, size, and shape of Te nanowires were found to be altered during exposure of cells, due to the instability and agglomeration of nanowires in the culture medium. These results suggest that the chemical components of the DMEM medium significantly affect the stability of Te nanowires. In addition, TEM images revealed that necrosis was the basic pattern of cell death, which might stem from the formation of toxic moieties of tellurium, released from nanowire structures, in the bioenvironment. These observations thus suggest that Te nanomaterials may pose potential risks to environmental and human health.

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Organoselenium and organotellurium compounds: Toxicology and pharmacology

Nogueira

Rocha

2011

Patai's Chemistry of Functional Groups

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In the last three decades, the interest in the field of synthesis and reactivity of organoselenium and organotellurium compounds has increased; particularly, in view of the observations that they can exhibit important biological activities. The data presented in this chapter clearly indicate the potential pharmacological and therapeutic uses of organoselenium and organotellurium compounds. Indeed, these classes of molecules exhibit a variety of interesting biological effects, namely antioxidant properties, which can account for their in vitro and in vivo beneficial effects in a wide range of models of different human pathologies. We can conclude that the future of “medicinal chemistry“ of organoselenium and organotellurium compounds will depend on the rational development of new molecules, which can be guided by chemical and biological approaches. Moreover, the structure‐activity relationship for a given class of organoselenium or organotellurium compounds should be used as a toll for screening molecules with high probability of exhibiting low toxicity and high pharmacological activity in mammals.

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Cytometric determination of novel organotellurium compound toxicity in a promyelocytic (HL-60) cell line

Cited by 37 publications

References 38 publications

Diphenyl Ditelluride‐Induced Cell Cycle Arrest and Apoptosis: A Relation with Topoisomerase I Inhibition

Diphenyl Ditelluride‐Induced Cell Cycle Arrest and Apoptosis: A Relation with Topoisomerase I Inhibition

Cytotoxic Potentials of Tellurium Nanowires in BALB/3T3 Fibroblast Cells

Organoselenium and organotellurium compounds: Toxicology and pharmacology

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