Cytometric and histopathologic features of tumors detected in a randomized mammography screening program: Correlation and relative prognostic influence

Hatschek, Thomas; Gröntoft, Otto; Fagerberg, G; Stål, Olle; Sullivan, S.; Carstensen, John; Nordenskjöld, Bo

doi:10.1007/bf01806352

Cited by 22 publications

(8 citation statements)

References 37 publications

(27 reference statements)

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“…The S phase percentage reflects the degree to which cells are proliferating and also serves as a marker for biologic activity by distinguishing tissues of high and low proliferation rates. Increased proliferative activity, as shown by the S phase percentage, has been associated with disease progression in breast cancer [17][18][19][20][21][22]. Similar to that reported for breast carcinomas, our data show that tissue containing benign atypical or hyperplastic proliferative changes or other benign histologic changes may be associated with either low or high cellular proliferative activity determined by S phase percentage.…”

Section: Discussionsupporting

confidence: 88%

Cellular proliferative activity of mammographic normal dense and fatty tissue determined by DNA S phase percentage

Stomper

Penetrante

Edge

et al. 1996

Breast Cancer Res Tr

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A prospective study was undertaken to evaluate associations among mammographic normal dense and fatty tissue, benign histologic findings, and cellular proliferative activity determined by DNA S phase percentages. Mammographic and histologic findings of benign, normal dense and fatty tissue contained in 100 excised specimens were cross-tabulated with flow cytometric DNA S phase percentages of needle aspirates obtained under radiographic guidance. Histologic evidence of atypia or hyperplasia was present in 21 (32%) of 66 fatty tissue samples and 11 (32%) of 34 dense tissue samples (p = NS). The median S phase percentages were identical for mammographic fatty and dense tissue (median % S phase = 5.35). The frequency of high S phase percentages (above the median) was not significantly different for tissue containing atypia or hyperplasia, 47% (15 of 32), as compared to tissue containing other benign histology, 52% (35 of 68) (p = NS). These frequencies were similar in women 49 and younger or 50 and older. These data show that mammographic normal dense and fatty tissues contain similar frequencies of histologic atypia or hyperplasia and low and high cellular proliferative activity determined by DNA S phase percentages in women aged 49 and younger and 50 and older.

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Section: Discussionsupporting

confidence: 88%

Cellular proliferative activity of mammographic normal dense and fatty tissue determined by DNA S phase percentage

Stomper

Penetrante

Edge

et al. 1996

Breast Cancer Res Tr

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“…Another method for assessing proliferative activity is the evaluation of mitotic activity. We are aware of seven studies that address this topic; all seven found a correlation between mitotic activity and SPF 18, 40–45. Nonetheless, mitotic activity was found to have independent prognostic value in studies46–48 as in the current one.…”

Section: Discussionmentioning

confidence: 77%

Synthesis of 3,3‐Disubstituted Oxindoles by Palladium‐Catalyzed Asymmetric Intramolecular α‐Arylation of Amides: Reaction Development and Mechanistic Studies

Katayev

Jia

Sharma

et al. 2013

Chemistry A European J

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Palladium complexes incorporating chiral N-heterocyclic carbene (NHC) ligands catalyze the asymmetric intramolecular α-arylation of amides producing 3,3-disubstituted oxindoles. Comprehensive DFT studies have been performed to gain insight into the mechanism of this transformation. Oxidative addition is shown to be rate-determining and reductive elimination to be enantioselectivity-determining. The synthesis of seven new NHC ligands is detailed and their performance is compared. One of them, L8, containing a tBu and a 1-naphthyl group at the stereogenic centre, proved superior and was very efficient in the asymmetric synthesis of fifteen new spiro-oxindoles and three azaspiro-oxindoles often in high yields (up to 99 %) and enantioselectivities (up to 97 % ee; ee=enantiomeric excess). Three palladacycle intermediates resulting from the oxidative addition of [Pd(NHC)] into the aryl halide bond were isolated and structurally characterized (X-ray). Using these intermediates as catalysts showed alkene additives to play an important role in increasing turnover number and frequency.

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“…Flow cytometric measurement of DNA content per nucleus provides information both about DNA ploidy status and about proliferative activity as represented by the fraction of cells in the S-phase of the cell cycle, The prognostic value of DNA ploidy has been demonstrated in many studies (6,8,9,20,24,42), as has that of the S-phase fraction (SPF; 13,15,16,22,31,37), though others have failed to confirm the independent prognostic value of these variables (12,21,30). sults.…”

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confidence: 99%

One or multiple samplings for flow cytometric DNA analyses in breast cancer‐prognostic implications?

Fernö¹,

Baldetorp²,

Ewers³

et al. 1992

Cytometry

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Flow cytometric assessments of DNA ploidy status and the S-phase fraction (SPF) have been shown to yield prognostic information in breast cancer. The aim of the present investigation was to elucidate the reproducibility of results with regard to tumor DNA heterogeneity, and to ascertain whether the prognostic value of DNA measurements might be enhanced by analyzing two pieces of a tumor instead of one.Agreement with regard to ploidy status (diploid versus non-diploid) was obtained in 90% of cases (71/79) when two adjacent sections of the tumor were investigated, and in 77% of cases (10/13) when four biopsies from different quadrants of the tumor specimen were investigated. The corresponding figures for agreement in SPF (divided into three categories, < 7.0%, 7.0-11.9%, and > 12%) were 75% 69/79; 2-sample series) and 55% (7/13; 4-biopsy series).The main reason for variance in ploidy results was the difficulties in distinguishing near diploid cell populations. Discrepancies in SPF categories could be explained by minor fluctuations in SPF values near the cut-off levels, or by variance in ploidy status, the fraction of nondiploid nuclei, and background noise due to cell debris.There was a stepwise increase in recurrence rate (RR) among patients with increasing SPF category (RR: 20%, 41%, and 53%). Patients whose SPF categories varied, from low or intermediate in one part of the tumor to high in another, seemed to have a poor prognosis (RR = 57%).We conclude that the variation in ploidy status and SPF between different parts of the same tumor specimen is largely to be explained by uncertainty in the interpretation of the DNA histograms and by difficulties inherent in the estimation of SPF, rather than by the existence of DNA tumor heterogeneity. Evaluation of a different part of the same tumor specimen may be of value, especially in the case of SPF, as this may yield additional prognostic information. These findings also emphasize the need for good quality control of FCM DNA analysis.

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Cytometric and histopathologic features of tumors detected in a randomized mammography screening program: Correlation and relative prognostic influence

Cited by 22 publications

References 37 publications

Cellular proliferative activity of mammographic normal dense and fatty tissue determined by DNA S phase percentage

Cellular proliferative activity of mammographic normal dense and fatty tissue determined by DNA S phase percentage

Synthesis of 3,3‐Disubstituted Oxindoles by Palladium‐Catalyzed Asymmetric Intramolecular α‐Arylation of Amides: Reaction Development and Mechanistic Studies

One or multiple samplings for flow cytometric DNA analyses in breast cancer‐prognostic implications?

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