Cytomegalovirus, varicella-zoster virus, and Coxsackie B virus-specific IgM antibody responses in children with cancer

Morris, David J.; Grint, P.; Bell, Eleanor J.; Lomax, Joyce; McCartney, R.A.

doi:10.1016/0888-0786(90)90035-m

Cited by 8 publications

(2 citation statements)

References 9 publications

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“…Cytomegalovirus-specific immunoglobulin G and M (IgG and IgM) antibodies were measured by enzymelinked immunosorbent assay (ELISA) [13]. Patients and donors were screened for CMV-specific IgG prior to transplantation.…”

Section: Virologymentioning

confidence: 99%

Pulmonary diffusion impairment following heart transplantation: a prospective study

Egan

Lowe²,

Yonan³

et al. 1996

Eur Respir J

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The aim of this prospective study was to confirm whether and when a fall in gas transfer occurs following heart transplantation (HT); and to examine the potential relationship between gas transfer and haemodynamic change, immunosuppression, and cytomegalovirus (CMV) infection.The lung physiology of 34 heart transplant recipients (HTR) and 14 control patients undergoing coronary artery bypass grafting (CABG) were studied. The absolute and standardized residual values of forced expiratory volume in one second (FEV1), forced vital capacity (FVC), residual volume (RV), forced residual volume (FRC), total lung capacity (TLC), transfer factor of the lungs for carbon monoxide (TL,CO) and carbon monoxide transfer coefficient (KCO) were measured before and at 30, 60, 90, 120 and 150 days after HT. These data were compared to haemodynamic status, graft rejection, cyclosporin levels and episodes of CMV infection. Lung function was studied in a group of patients before and 4 weeks after CABG.There was a significant fall in mean KCO after HT (pre-HT=1.29 and post-HT= 1.06 mmol·min -1 ·kPa·L -1 ) but not after CABG (pre-CABG=1.49, post-CABG=1.5 mmol·min -1 ·kPa·L -1 . No relationship was observed between gas transfer and CMV.At the latest stage following HT (150 days) there was a positive correlation between TL,CO (absolute value and standardized residual) and mean cyclosporin level (r=0.48 and r=0.44, respectively) and also between the absolute KCO and actual (r=0.56) and mean (r=0.55) cyclosporin levels. Following HT, there is an early fall in gas transfer, which is independent of the effects of surgery and bypass, implicating early immunosuppression (e.g. antithymocyte globulin/cyclosporin).

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Section: Virologymentioning

confidence: 99%

Pulmonary diffusion impairment following heart transplantation: a prospective study

Egan

Lowe²,

Yonan³

et al. 1996

Eur Respir J

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“…CMV-specific IgM is a sensitive indicator of an ongoing or recent infection [9,12]. However, it is not a specific indicator of primary infection, as IgM can persist for up to 12 months after initial infection [13][14][15][16] and can be present during reactivation or reinfection in patients [17][18][19]. In addition, other infections, such as Epstein-Barr virus (EBV), can interfere with CMV-specific IgM measurements [20,21], resulting in false-positive results.…”

Section: Introductionmentioning

confidence: 99%

Clinical evaluation of new automated cytomegalovirus IgM and IgG assays for the Elecsys® analyser platform

Revello

Vauloup‐Fellous

Grangeot-Keros

et al. 2012

Eur J Clin Microbiol Infect Dis

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Cytomegalovirus (CMV) is a leading cause of physical and neurological abnormalities in newborns. Hence, the diagnosis of CMV infection in pregnant women is necessary in order to allow appropriate management of their pregnancy. New assays have been developed for the Roche Elecsys® immunoassay platform that detect CMV-specific immunoglobulin (Ig)M and IgG, with the IgM assay designed to target IgM produced at the start of infection rather than IgM persisting later in infection. This study aimed to evaluate the performance of the new assays compared with other commercial kits widely distributed in laboratories. The performance of the Elecsys and comparator CMV IgM and IgG assays was assessed using 967 preselected patient samples characterised by CMV infection status, as well as being compared using 1,668 unselected clinical samples. The Elecsys CMV IgM and IgG assays performed consistently with comparator assays using the preselected samples. The Elecsys CMV IgM assay showed improved sensitivity compared with the Enzygnost® assay in primary infection (91.2 % vs. 79.4 %) and improved specificity over the Architect® assay in potentially cross-reacting samples (94.1 % vs. 82.4 %). The Elecsys IgM assay reported fewer positive results in the later stages of CMV infection compared with ETI-CYTOK-M ELISA, while the Elecsys IgG assay reported slightly fewer negative results in the early stages of infection compared with ETI-CYTOK-G ELISA. There was good agreement between Elecsys and comparator assays using unselected clinical samples (range 90.4-99.4 %). The Elecsys CMV IgM and IgG assays compare well with routinely used assays and are suitable for clinical use.

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Comparative study of three PCR assays with antigenaemia and serology for the diagnosis of HCMV infection in thoracic transplant recipients

et al. 1996

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Three PCR assays were evaluated for the detection of human cytomegalovirus (HCMV) infection in heart and lung transplant recipients in comparison with HCMV antigenaemia and serology assay. Polymorphonuclear leucocyte (PMNL) samples taken at regular intervals after transplantation were tested for HCMV DNA using primer sets homologous to the glycoprotein B (gp58), major immediate early (IE1), and structural phosphoprotein (pp150) regions. The detection of HCMV infection at various times after transplantation showed all three primer sets to have a sensitivity of 100% and a specificity of 92.3% for the detection of HCMV infection although overall the gp58 primer set was found to be significantly more frequently associated with a positive PCR result than the IE1 (P = 0.0228) and pp150 (P = 0.0015) primer sets. The positive PCR result had a positive predictive value of 27.8% for HCMV disease. Detection of HCMV infection was first by the PCR assay, and significantly before the HCMV antigenaemia assay. Of nine patients who received antiviral therapy while PCR positive, only one patient cleared HCMV DNA from PMNLs during treatment but became positive again 17 days later. Quantitative PCR methodologies may improve the predictive value of PCR for HCMV disease and its value for monitoring antiviral therapy.

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Cytomegalovirus, varicella-zoster virus, and Coxsackie B virus-specific IgM antibody responses in children with cancer

Cited by 8 publications

References 9 publications

Pulmonary diffusion impairment following heart transplantation: a prospective study

Pulmonary diffusion impairment following heart transplantation: a prospective study

Clinical evaluation of new automated cytomegalovirus IgM and IgG assays for the Elecsys® analyser platform

Comparative study of three PCR assays with antigenaemia and serology for the diagnosis of HCMV infection in thoracic transplant recipients

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