Cytomegalovirus infection increases the expression and activity of ecto‐ATPase (CD39) and ecto‐5′nucleotidase (CD73) on endothelial cells

Kas-Deelen, A.M.; Bakker, Winston W.; Olinga, Peter; Visser, Joost; Maar, Eltjo F. de; Son, Willem J. van; The, T. H.; Harmsen, Martin C.

doi:10.1016/s0014-5793(01)02085-3

Cited by 30 publications

(29 citation statements)

References 29 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Levels of antioxidants enzymes in platelets of different study groups: SOD and CAT activities in the platelets of type 2 DM subjects and control group (data are expressed as mean ± SEM).There were no significant differences, as determined by Student's t-test. A B interest, the metabolism of extracellular nucleotides plays an important regulatory role in the control of adequate hemostasis, mainly by regulating platelet coagulant status [61]. In the present study, we observed an increase of 5'NT activity in diabetic platelets (Fig.…”

Section: Discussionsupporting

confidence: 63%

<i>Syzygium cumini</i> Extract Decrease Adenosine Deaminase, 5′Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients

Bona

Bellé

Sari

et al. 2010

Cell Physiol Biochem

View full text Add to dashboard Cite

Diabetes mellitus, a chronic metabolic disorder, has assumed epidemic proportions and its long-term complications can have devastating consequences. The oxidative stress in diabetes was greatly increased due to prolonged exposure to hyperglycemia and impairment of oxidant/antioxidant equilibrium. Syzygium cumini is being widely used to treat diabetes by the traditional practitioners over many centuries. Adenosine deaminase (ADA) and 5′-Nucleotidase (5′NT) are enzymes of purine nucleoside metabolism that play an important role in the regulation of adenosine (Ado) levels. In this study, we investigated the effect of Syzygium cumini aqueous leaves extract (ASc) on ADA and 5′NT activities and on parameters of oxidative stress under in vitro conditions, using platelets of patients with Type 2 diabetes mellitus. Platelet-Rich Plasma (PRP) was assayed by ADA, 5′NT, Catalase (CAT), Superoxide Dismutase (SOD) activities and Thiobarbituric acid reactive substances (TBARS) levels. We observed that ADA, 5′NT activities and TBARS levels were significantly higher when compared to the control group, and ASc (100 and 200 µg/mL) prevented these effects. Our study demonstrates that ASc was able to remove oxidant species generated in diabetic conditions and modulates in the Ado levels. Then, ASc may promote a compensatory response in platelet function, improving the susceptibility-induced by the diabetes mellitus.

show abstract

Section: Discussionsupporting

confidence: 63%

<i>Syzygium cumini</i> Extract Decrease Adenosine Deaminase, 5′Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients

Bona

Bellé

Sari

et al. 2010

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Previously, we observed that infection of human endothelial cells in vitro with CMV leads to increased activity of ectoenzymes, such as ecto-nucleotide triphosphate diphosphohydrolase (ENTPD)-1, also known as ectoapyrase or CD39, and ecto-5Јnucleotidase (CD73) (6). It has been shown that ENTPD1 is a protective factor in vascular injury, including reperfusion injury models and transplant rejection (7)(8)(9).…”

mentioning

confidence: 99%

“…It has been shown that ENTPD1 is a protective factor in vascular injury, including reperfusion injury models and transplant rejection (7)(8)(9). Thus, this ecto-enzyme is able to hydrolyze adenosine triphosphate (ATP) through adenosine diphosphate (ADP) to adenosine monophosphate (AMP), whereas AMP can be hydrolyzed to adenosine by CD73 (6). Local extracellular ATP and ADP show proinflammatory and proaggregatory properties, respectively (10,11).…”

mentioning

confidence: 99%

Infection of Human Endothelium In Vitro by Cytomegalovirus Causes Enhanced Expression of Purinergic Receptors: A Potential Virus Escape Mechanism?

Zandberg

Son²,

Harmsen

et al. 2007

Transplantation

View full text Add to dashboard Cite

Infection of endothelium with CMV causes significant upregulation of the P2 receptors studied. As these receptors may potentially be able to concentrate nucleotides along the ectonucleotidases of the endothelial cell membrane, rapid local hydrolysis of adenosine triphosphate and adenosine diphosphate may be facilitated by enhanced P2 receptor expression. Such a CMV induced mechanism might enable the virus to escape from an important host defense response, such as local microthrombus formation.

show abstract

“…Kas-Deelen et al [70] reported that cytomegalovirus infection induced increased expression and activity of ecto-NTPDase (CD39) and ecto-5'-nucleotidase (CD73) in endothelial cells. In this context, the production of adenosine is probably enhanced.…”

Section: Immunomodulation and Cell-cell Interactionsmentioning

confidence: 99%

NTPDase and 5'‐nucleotidase activities in physiological and disease conditions: New perspectives for human health

et al. 2007

View full text Add to dashboard Cite

Abstract. Extracellular nucleotides and nucleosides act as signaling molecules involved in a wide spectrum of biological effects. Their levels are controlled by a complex cell surface-located group of enzymes called ectonucleotidases. There are four major families of ectonucleotidases, nucleoside triphosphate diphosphohydrolases (NTPDases/CD39), ectonucleotide pyrophosphatase/phosphodiesterases (E-NPPs), alkaline phosphatases and ecto-5'-nucleotidase. In the last few years, substantial progress has been made toward the molecular identification of members of the ectonucleotidase families and their enzyme structures and functions. In this review, there is an emphasis on the involvement of NTPDase and 5'-nucleotidase activities in disease processes in several tissues and cell types. Brief background information is given about the general characteristics of these enzymes, followed by a discussion of their roles in thromboregulatory events in diabetes, hypertension, hypercholesterolemia and cancer, as well as in pathological conditions where platelets are less responsive, such as in chronic renal failure. In addition, immunomodulation and cell-cell interactions involving these enzymes are considered, as well as ATP and ADP hydrolysis under different clinical conditions related with alterations in the immune system, such as acute lymphoblastic leukemia (ALL), Bchronic lymphocytic leukemia (B-CLL) and infections associated with human immunodeficiency virus (HIV). Finally, changes in ATP, ADP and AMP hydrolysis induced by inborn errors of metabolism, seizures and epilepsy are discussed in order to highlight the importance of these enzymes in the control of neuronal activity in pathological conditions. Despite advances made toward understanding the molecular structure of ectonucleotidases, much more investigation will be necessary to entirely grasp their role in physiological and pathological conditions.

show abstract

Cytomegalovirus infection increases the expression and activity of ecto‐ATPase (CD39) and ecto‐5′nucleotidase (CD73) on endothelial cells

Cited by 30 publications

References 29 publications

<i>Syzygium cumini</i> Extract Decrease Adenosine Deaminase, 5′Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients

<i>Syzygium cumini</i> Extract Decrease Adenosine Deaminase, 5′Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients

Infection of Human Endothelium In Vitro by Cytomegalovirus Causes Enhanced Expression of Purinergic Receptors: A Potential Virus Escape Mechanism?

NTPDase and 5'‐nucleotidase activities in physiological and disease conditions: New perspectives for human health

Contact Info

Product

Resources

About