Cytomegalovirus infection in a volunteer blood donor population

Kane, R C; Rousseau, Wyatt E.; Noble, Gary R.; Tegtmeier, Gary E.; Wulff, Herta; Herndon, H B; Chin, Theresa; Bayer, William L.

doi:10.1128/iai.11.4.719-723.1975

Cited by 74 publications

(7 citation statements)

References 23 publications

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“…It follows, therefore, that both procedures could be used with confidence for screening sera for these applications. It should be emphasized that the calculations of predictive value performed in this study should be applicable to general laboratory practice, since the prevalence of CMV antibody in our sample (i.e., 44.8%) closely approximates that reported for the general population (i.e., 40 to 70%) (2,3,6).…”

Section: Methodssupporting

confidence: 69%

Detection of cytomegalovirus antibody with two commercially available assays, an indirect hemagglutination test and an enzyme immunosorbent assay

Cheeseman¹,

Doern²,

Ferriani³

et al. 1984

J Clin Microbiol

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By the use of two reference procedures, an indirect hemagglutination assay and a complement fixation test, the presence or absence of cytomegalovirus (CMV) antibody was determined for 221 human sera. Ninety-nine sera (44.8%) were found to contain CMV antibody. The remaining 122 sera (55.2%) lacked detectable CMV antibody. These same sera were then analyzed by two recently introduced, commercially available CMV antibody assays, an indirect hemagglutination test (IHA-c; Cetus Corp., Emeryville, Calif.) and an enzymelinked immunosorbent assay (ELISA; M. A. Bioproducts, Walkersville, Md.). With the results of the reference procedures as true evidence of the presence or absence of CMV antibody, the sensitivity of the IHA-c was found to be 100%; the specificity was 98.4%. The sensitivity of the ELISA was also 100%; the specificity was 96.7%. The overall accuracies of these procedures were 99.1 and 98.2%, respectively. Time and motion studies revealed the IHA-c procedure to be faster and technically less demanding than the ELISA procedure.

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Section: Methodssupporting

confidence: 69%

Detection of cytomegalovirus antibody with two commercially available assays, an indirect hemagglutination test and an enzyme immunosorbent assay

Cheeseman¹,

Doern²,

Ferriani³

et al. 1984

J Clin Microbiol

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“…Extraction of nucleic acids from serum. Nucleic acids were extracted from serum as described previously ( 14- (14) Urine; PCR + (14) and +(44); the urines Mother had a primary HCMV infection were bacterially infected, and no virus during the first trimester isolation was possible + (44) - 20Trace (20) a Numbers within parentheses indicate the days on which the serum samples were collected.…”

Section: Methodsmentioning

confidence: 99%

Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections

et al. 1992

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Using a specific and sensitive polymerase chain reaction method, we detected reliably the presence of human cytomegalovirus (HCMV) DNA directly in serum samples collected at an early stage of HCMV infection, even before immunoglobulin M (IgM) antibodies were measurable. HCMV DNA was detected in serum from all patients with active HCMV infection; in 91% of these patients, HCMV DNA was found in the acute-phase serum. In 13 of 44 patients, HCMV DNA was found in serum before HCMV-specific IgM. For four kidney transplant recipients, the occurrence of HCMV DNA in serum, virus isolation from urine and leukocytes, and HCMV IgG and IgM serology were determined. We found a correlation between HCMV DNA in serum and positive virus isolation from leukocytes. In three of five congenitally infected infants, HCMV DNA and HCMV IgM were detected in the same sample. Two other infants were HCMV DNA positive, although no HCMV IgM antibodies were measurable. HCMV was found in urine from these infants either by virus isolation or with the polymerase chain reaction. Serum from one of the 22 healthy HCMV-seropositive blood donors was HCMV polymerase chain reaction positive.

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“…Jedoch wurde die Prävalenz der CMV-Infektion in einem Land umgekehrt proportional dem sozioökonomischen Status gefunden. Der CMV-Durchseuchungsgrad der Blutspender hängt vom Alter und Geschlecht ab (14,24). Die höchste Frequenz wurde bei 65 Jahre alten Spendern gefunden, wobei Frauen höhere CMV-Antikörper-Prävalenzen aufwiesen als Männer.…”

Section: Diskussionunclassified

Blutspenderauswahl zur Bereitstellung von anti-Cytomegalievirus-negativen Blutkomponenten

Koerner

Freudenberg²,

Kubanek³

2008

Dtsch med Wochenschr

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Sera of 1731 blood donors of the Ulm blood bank were screened for cytomegalovirus (CMV) antibodies. Prevalence of these antibodies was 37%. The seroconversion rate among 1090 anti-CMV-negative blood donors was 13 per thousand per year for the whole donor population over a period of 27 months and depended on age. It was 16 per thousand per year for donors aged 18-40 years, but only 7 per thousand for those over 41 years old. Seropositivity was higher in women than men. To provide anti-CMV-negative blood requires a prescreened anti-CMV-negative donor group and the rescreening of each further donated blood component.

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Cytomegalovirus infection in a volunteer blood donor population

Cited by 74 publications

References 23 publications

Detection of cytomegalovirus antibody with two commercially available assays, an indirect hemagglutination test and an enzyme immunosorbent assay

Detection of cytomegalovirus antibody with two commercially available assays, an indirect hemagglutination test and an enzyme immunosorbent assay

Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections

Blutspenderauswahl zur Bereitstellung von anti-Cytomegalievirus-negativen Blutkomponenten

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