Cytomegalovirus glycoprotein B and N genotypes in pediatric recipients of the hematopoietic stem cell transplant

Janković, Marko; Ćupić, Maja; Knežević, Aleksandra; Vujić, Dragana; Soldatović, Ivan; Zecevic, Zeljko; Gobeljić, Borko; Jovanović, Tanja

doi:10.1016/j.virol.2020.07.010

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…In the present study, the most prevalent genotypes were gB1 and gB4, followed by gB3, gB2, and gB5 (Figure 1). This is in accordance with our previous results obtained from pediatric patients that underwent HSCT, wherein the gB1 and gB4 variants were the most predominant as well [67].…”

Section: Discussionsupporting

confidence: 93%

Significance of Cytomegalovirus gB Genotypes in Adult Patients Undergoing Hematopoietic Stem Cell Transplantation: Insights from a Single-Centre Investigation

Vasiljevic,

Jankovic,

Tomic

et al. 2024

Pharmaceuticals

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Introduction: Cytomegalovirus (CMV) infection is a major clinical issue after allogeneic hematopoietic stem cell transplantation (HSCT). The CMV envelope glycoproteins are key in viral pathogenesis; the glycoprotein B (gB) encoded by the UL55 gene might be an important determinant of viral virulence and disease severity marker in patients treated with allogeneic HSCT. Our aim was to investigate the molecular diversity of CMV gB and inquire into the associations between UL55 gene variations and clinical manifestations in adult patients treated with allogeneic HSCT. Results: The most prevalent genotypes were gB1 and gB4 (11/27, 40.7%). Patients with genotype gB1 infection had earlier platelet engraftment (p < 0.033) and less frequent minimal/measurable residual disease post HSCT than those without this genotype. Patients with gB4 glycoprotein infection had a significantly lower CD4+/CD8+ ratio at D90 (p < 0.026). Interestingly, patients with gB5 glycoprotein infection had shorter overall survival from base condition diagnosis (p < 0.042), as well as shorter overall survival after HSCT (p < 0.036). Acute GvHD was noted more frequently in those with mixed-genotype infection (p = 0.047). Material and Methods: The study included fifty-nine adult patients treated with allogeneic HSCT. Peripheral venous blood was sampled typically per week, with detection of CMV performed by quantitative real-time PCR. Multiplex nested PCR was used to determine specific gB genotypes, which were then statistically compared vis-à-vis specific clinical variables. Conclusions: Our study points to variations in the viral UL55 locus imparting both beneficial (earlier platelet engraftment, less frequent MRD post HSCT) and adverse effects (shorter overall survival, more frequent acute GvHD, less frequent 100% chimerism at day 90) to the transplanted host. Comprehensive molecular investigations are necessary to validate this apparent duality, as the potential benefits of CMV could perhaps be utilized for the benefit of the patient in the future.

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Section: Discussionsupporting

confidence: 93%

Significance of Cytomegalovirus gB Genotypes in Adult Patients Undergoing Hematopoietic Stem Cell Transplantation: Insights from a Single-Centre Investigation

Vasiljevic,

Jankovic,

Tomic

et al. 2024

Pharmaceuticals

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“…HCMV can be classified into four genotypes (gB1 to gB4) based on the sequence variations in the N-terminus of the gB gene. These gB genotypes may be associated with different clinical symptoms and outcomes [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…Demonstration of HCMV DNA has emerged as the diagnostic criteria for HCMV infection [16], for PCR assays' high sensitivity and specificity. In particular, quantification of the HCMV DNA copies is a powerful means for assaying viral activity, evaluating antiviral efficacy, and determining the severity of infection [13,17]. Blood, urine, and saliva can be used as samples for PCR screening of HCMV DNA, but their detection rates are different [14].…”

Section: Introductionmentioning

confidence: 99%

Human cytomegalovirus glycoprotein B genotypic distributions and viral load in symptomatic infants

Mu,

Qiao,

Zou

et al. 2023

J Infect Dev Ctries

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Introduction: HCMV infection is widespread in humans. This retrospective study aimed to explore the relationship between human cytomegalovirus (HCMV) glycoprotein B (gB) genotype distribution, viral load, and the demographic and clinical features of symptomatic infants. The detection rate of HCMV in blood and urine samples was also compared. Methodology: Retrospective data from 265 infants who underwent urine HCMV DNA testing were analyzed. The viral load and gB genotype were detected in 91 HCMV positive infants by quantitative fluorescence polymerase chain reaction (PCR) and DNA sequencing, respectively. Results: The positive rate of HCMV infection was 46.04% (122/265) in all infants, and increased rapidly with age. Among the 91 infants investigated, liver function abnormality was the most common diagnosis (34/91, 37.36%), followed by pneumonia (21/91, 23.07%). Sequence analysis of gB yielded two genetic subtypes: the most prevalent gB3 (47/91, 51.65%), followed by gB1 (44/91, 48.35%). The gB3 HCMV infection was more prevalent in infants aged 0-2 months than in infants aged 3-12 months (χ2 = 4.38, p = 0.0364). The data showed that ALT and AST levels were significantly higher in the anti-HCMV IgM+IgG– group than in the anti-HCMV IgM+IgG+ and IgM-IgG+ groups. In addition, this study showed that the detection rate of HCMV DNA in the blood was significantly lower than that in the urine (χ2 = 6.7131, p = 0.0096). Conclusions: This study presents the HCMV infection status of infants and its relationship with their demographic characteristics and clinical manifestations. In addition, this study suggests that urinary PCR is the most appropriate assay for detecting HCMV infections.

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Pediatric hematopoietic stem cell transplantation in Serbia - 25 years of experience

et al. 2022

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The first allogeneic identical sibling donor hematopoietic stem cell transplantation at the Institute for Mother and Child Health Care of Serbia ?Dr. Vukan Cupic? (Institute) was performed in the seventies of the last century. Almost twenty years later, the first allogeneic transplant from sibling haploidentical donor was performed in an infant suffering from severe combined immunodeficiency. After several years of efforts, the bone marrow transplant unit was opened in April 1997 at the Institute. Over the 25 years, the transplant team performed 360 hematopoietic stem cell transplantations, 175 autologous and 185 allogeneic (103 from identical sibling donor, 43 from haploidentical sibling donor, 36 from matched unrelated donor, 2 from identical sibling cord blood and 1 from unrelated identical cord blood).

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Cytomegalovirus glycoprotein B and N genotypes in pediatric recipients of the hematopoietic stem cell transplant

Cited by 5 publications

References 32 publications

Significance of Cytomegalovirus gB Genotypes in Adult Patients Undergoing Hematopoietic Stem Cell Transplantation: Insights from a Single-Centre Investigation

Significance of Cytomegalovirus gB Genotypes in Adult Patients Undergoing Hematopoietic Stem Cell Transplantation: Insights from a Single-Centre Investigation

Human cytomegalovirus glycoprotein B genotypic distributions and viral load in symptomatic infants

Pediatric hematopoietic stem cell transplantation in Serbia - 25 years of experience

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