Cytomegalovirus (CMV) glycoprotein B genotype and CMV DNA load in the amniotic fluid of infected fetuses

Picone, Olivier; Costa, Jean‐Marc; Leruez‐Ville, Marianne; Ernault, Pauline; Olivi, Martine; Ville, Y.

doi:10.1002/pd.942

Cited by 70 publications

(66 citation statements)

References 24 publications

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“…It is therefore not relevant to test for UL144 genotypes in this context. Other HCMV polymorphisms, such as glycoprotein B genotypes, have been evaluated in congenital infection and also proved to be disappointing (2,3,5,13,15,21). Moreover, it was recently demonstrated that the inter-and intragenic variability of HCMV clinical isolates leads to an infinite number of genetic combinations, probably explaining the failure to use sequence information to predict disease outcome (16).…”

Section: Discussionmentioning

confidence: 99%

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Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

Picone

Costa

Chaix³

et al. 2005

J Clin Microbiol

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The human cytomegalovirus (HCMV) UL144 gene is a tumor necrosis factor-like receptor with the potential to affect HCMV virulence. HCMV strains display genetic variability in the UL144 region, and the analysis of a potential link between UL144 gene polymorphisms and disease severity has scarcely been studied. However, a correlation between the UL144 genotype and congenital-disease outcome has been reported in one previous study, with the observation that all asymptomatic infants had a single UL144 genotype. In order to confirm or refute this finding, we determined the UL144 polymorphisms of HCMV strains recovered from the amniotic fluids of 38 infected fetuses and compared them to HCMV strains obtained from 30 viremic adult controls. The UL144 sequences were distributed among five genotypes (A, B, C, AC, and AB), as previously described. We observed similar percentages of the three major genotypes A (37%), B (33%), and C (27%) in our population. The UL144 genotype distributions were similar among the group of infected adults and the group of infected fetuses and among symptomatic and asymptomatic fetuses (P < 0.05). In our series, all five UL144 genotypes could be vertically transmitted from mothers to fetuses, and all could cause symptomatic congenital infection. We concluded that determination of UL144 polymorphisms in cases of congenital infection is not relevant, since it is unlikely to help predict the outcome of the infection.

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Section: Discussionmentioning

confidence: 99%

“…Ultrasonographic reports, outcome, and/or postmortem examination reports were collected. Some of these cases have also been described elsewhere (15). We classified the fetuses into two groups.…”

Section: Patients and Samples Between February 2001 And May 2002mentioning

confidence: 99%

Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

Picone

Costa

Chaix³

et al. 2005

J Clin Microbiol

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“…In addition, some of the methods previously described are not easily applied to high-throughput monitoring. Based on Chou's classification of gB genotypes, several methods of genotyping CMV gB were adapted, including restriction fragment analysis (RFA) of PCR products (16,20,34), conventional multiple nested PCR (4,28,38), and direct DNA sequencing (24). All of these methods either lack sensitivity or are labor intensive and/or nonquantifiable.…”

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confidence: 99%

Concurrent Genotyping and Quantitation of Cytomegalovirus gB Genotypes in Solid-Organ-Transplant Recipients by Use of a Real-Time PCR Assay

2008

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We have developed a real-time genotyping and quantitative PCR (RT-GQ-PCR) assay to genotype cytomegalovirus (CMV) and quantify viral loads simultaneously in solid organ transplant (SOT) recipients. Special minor-groove DNA-binding probes were designed based on sequence polymorphism in the gB gene to increase genotyping specificity for gB1 to gB4. For validation, 28 samples with known genotypes determined by restriction fragment analysis (RFA) and 121 with unknown genotypes were tested. All samples were from SOT patients with CMV viremia. A 100% concordance for genotyping was achieved by using the RT-GQ-PCR with known genotypes determined by RFA. The RT-GQ-PCR identified more cases of CMV infections with mixed genotypes than RFA did. No cross-reaction between genotypes was observed. All four gB genotypes were detected in the 121 samples of unknown genotype. gB1 was the predominant single genotype (n ‫؍‬ 61, 50.4%), followed by gB2 (n ‫؍‬ 26, 21.0%), gB3, (n ‫؍‬ 11, 9.1%), and gB4 (n ‫؍‬ 3, 2.5%). Mixed-genotype infections were detected in 17% (20/121) of the samples. Patients with mixed-genotype infections had significantly higher CMV viral loads than those with single-genotype infections (P ‫؍‬ 0.019). The RT-GQ-PCR assay was found to be highly sensitive and specific, with a wide dynamic range (2.7 to 10.7 log 10 copies/ml) and very good precision (coefficient of variation, ϳ1.78%). With the prominent feature of concurrent CMV gB genotyping and quantitation in a single reaction, the new assay provides a rapid and cost-effective method for monitoring CMV infection in SOT recipients.

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“…Although CMV infection is diagnosed when detected in amniotic fluid by PCR, there is no relationship between the CMV gene dosage in the amniotic fluid and neonatal prognosis [18]. The CMV-DNA level in umbilical cord venous blood was used to diagnose the presence of the viral infection; however, it did not appear to be a suitable indicator of therapeutic effect.…”

Section: Extent Of Fetal CMV Invasionmentioning

confidence: 99%

The Value of Neuron-specific Enolase and Cytokines from Umbilical Blood in Cytomegalovirus Infected Fetuses

Matsuda¹

2013

J Mycobac Dis

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Cytomegalovirus (CMV) glycoprotein B genotype and CMV DNA load in the amniotic fluid of infected fetuses

Abstract: Neither gB genotype nor CMV DNA load in AF correlate with the severity of CMV congenital infection and these markers are unlikely to prove useful for the management of congenital infection.

Cited by 70 publications

References 24 publications

Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

Concurrent Genotyping and Quantitation of Cytomegalovirus gB Genotypes in Solid-Organ-Transplant Recipients by Use of a Real-Time PCR Assay

The Value of Neuron-specific Enolase and Cytokines from Umbilical Blood in Cytomegalovirus Infected Fetuses

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