Cytomegalovirus and Epstein–Barr virus DNA transcription in endodontic symptomatic lesions

Sabeti, Mohammad; Vallès, Yvonne; Nowzari, Hessam; Simon, James H.; Kermani-Arab, Vali; Slots, Jørgen

doi:10.1034/j.1399-302x.2003.00055.x

Cited by 89 publications

(138 citation statements)

References 45 publications

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“…Infection by HCMV and EBV has been more frequently observed in symptomatic lesions (173,174), and an association has been suggested. However, data related to the occurrence of herpesviruses in acute apical abscesses are rather inconclusive.…”

Section: The Host Side Of the Storymentioning

confidence: 99%

Microbiology and Treatment of Acute Apical Abscesses

2013

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SUMMARY Acute apical abscess is the most common form of dental abscess and is caused by infection of the root canal of the tooth. It is usually localized intraorally, but in some cases the apical abscess may spread and result in severe complications or even mortality. The reasons why dental root canal infections can become symptomatic and evolve to severe spreading and sometimes life-threatening abscesses remain elusive. Studies using culture and advanced molecular microbiology methods for microbial identification in apical abscesses have demonstrated a multispecies community conspicuously dominated by anaerobic bacteria. Species/phylotypes commonly found in these infections belong to the genera Fusobacterium , Parvimonas , Prevotella , Porphyromonas , Dialister , Streptococcus , and Treponema . Advances in DNA sequencing technologies and computational biology have substantially enhanced the knowledge of the microbiota associated with acute apical abscesses and shed some light on the etiopathogeny of this disease. Species richness and abundance and the resulting network of interactions among community members may affect the collective pathogenicity and contribute to the development of acute infections. Disease modifiers, including transient or permanent host-related factors, may also influence the development and severity of acute abscesses. This review focuses on the current evidence about the etiology and treatment of acute apical abscesses and how the process is influenced by host-related factors and proposes future directions in research, diagnosis, and therapeutic approaches to deal with this disease.

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Section: The Host Side Of the Storymentioning

confidence: 99%

Microbiology and Treatment of Acute Apical Abscesses

2013

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“…The fact that HCMV was detected as a single infection in 24% of all the periapical lesions while no lesions had EBV monoinfection shows that the HCMV endodontic pathogen is perhaps more important. A study by Sabeti and Slots [24] described only one periapical lesion showing EBV monoinfection, while Sabeti et al [25] found HCMV active infection in 92% and EBV infection in 62% of symptomatic periapical lesions. Approximately the same ratio of these two viruses was found by Yildirim et al [26], who examined the presence of herpesvirus in periapical lesions of deciduous teeth.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions

Popović

Gasic²,

Živković³

et al. 2015

Intervirology

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Objective: The aim of this study was to investigate the presence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the tissue of chronic periapical lesions, and to compare the results in relation to the symptoms of patients and the size of the lesion. Methods: Periapical lesions analyzed in the study were collected from the roots of the teeth indicated for extraction. Samples were divided according to the symptoms into groups of symptomatic and asymptomatic, and according the size into groups of small and large lesions. Polymerase chain reaction was used to detect HCMV and EBV. The amplification was performed in a DNA Thermal Cycler (Hybaid). Results: Symptomatic lesions were 7.68 times more likely to be infected with HCMV than asymptomatic lesions (p < 0.001). Large symptomatic lesions were 73.50 times more likely to harbor HCMV than small symptomatic lesions (p < 0.001). Large symptomatic lesions were 7.64 times more likely to be infected with EBV than small symptomatic lesions (p = 0.05). Large symptomatic lesions were 5.38 times more likely to harbor dual HCMV/EBV infection than small symptomatic lesions (p = 0.115). Conclusion: Detection of HCMV and EBV in the samples of periapical lesions suggests an important role of herpesviruses in periapical tissue destruction.

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“…In another study, Ferreira et al [18] found EBV in 6% of purulent exudates from acute apical abscesses, whereas they did not identify HCMV in abscess samples using single and nested PCR. The discrepancies in the findings among our study and the three studies mentioned above might arise for several reasons, including differences in the primers used, methods used for DNA extraction, and host and environmental factors such as genetic background, ethnicity, and socioeconomic status [8,[20][21][22][23]. We used same sampling techniques applied by Chen et al [1].…”

Section: Discussionmentioning

confidence: 99%

Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses

Ozbek¹

2015

J Dent Probl Solut

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Introduction: To investigate the possible association between presence of Epstein-Barr virus, human cytomegalovirus and three endodontic bacterial pathogens (Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) "red complex" in samples from patients with acute apical abscesses using real-time PCR.Methods: Periapical samples were collected from 18 acute apical abscess specimens and 6 control samples. The apical area of each study tooth was examined in periapical radiographs obtained using a long cone paralleling technique. The study included only single-rooted teeth that had carious lesions, necrotic pulps, and radiographic evidence of periradicular bone loss. No apparent communication from the abscess to the oral cavity or skin surface was observed. DNA extracts from purulent exudate aspirates of acute apical abscess and six control samples were evaluated for the presence of microbial and viral loads using real-time polymerase chain reaction (PCR) following the kit protocols recommended by the manufacturers. The chi squared test was used for statistical analysis.Results: HCMV, EBV, T. denticola, P. gingivalis, and T. forsythia were detected in 16.7 %, 5.6 %, 44.4 %, 27.7 %, and 22.2 % of the samples, respectively. As for the healthy pulps used as noninflamed controls, no control specimens contained bacterial or viral DNA. The pair HCMV/ T. denticola and HCMV/ T. forsythia was detected in one case. The association between HCMV, T denticola and T. forsythia was not statistically significant. Conclusion:Within the limitations of the present study, our PCR based findings revealed that in "red complex" bacteria, T. denticola and T. forsythia tended to occur in co-infection with HCMV.

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Cytomegalovirus and Epstein–Barr virus DNA transcription in endodontic symptomatic lesions

Cited by 89 publications

References 45 publications

Microbiology and Treatment of Acute Apical Abscesses

Microbiology and Treatment of Acute Apical Abscesses

Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions

Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses

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