Cytolytic T cells recognize the two amino-terminal domains of H-2 K antigens in tandem in influenza A infected cells

Arnold, Bernd; Burgert, Hans‐Gerhard; Hamann, Ute; Hämmerling, G. J.; Kees, Ursula R.; Kvist, Sune

doi:10.1016/0092-8674(84)90528-2

Cited by 77 publications

(46 citation statements)

References 31 publications

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“…We have previously shown that CTL generated during influenza A infection recognize determinants made up of the (1985) al and a2 domains of the H-2Kd and H-2Kk antigens (7). Expression of the al and a2 domains of the same allele was the requirement for efficient lysis of the infected cells.…”

Section: Discussionmentioning

confidence: 99%

“…The H-2Kd gene and the H-2Kk gene have been characterized extensively (8,9). The construction of hybrid genes, plasmids pC31, pC32, and pC33, from the H-2Kd and H-2Kk genes has been described in detail recently (7). We used the same deletion subcloning method (7) to construct more hybrid genes, plasmids pC23, pC24, and pC25 (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Cells were grown in Dulbecco's minimal essential medium containing 10% fetal calf serum. H-2-specific DNA was cotransfected with the neophosphotransferase gene using the calciumphosphate method as described (7).…”

Section: Methodsmentioning

confidence: 99%

“…Anti-H-2Dd CTL were able to recognize efficiently an H-2Dd/Ld hybrid with only the al domain of H-2Dd (5). In contrast, exchange of al between H-2Kb/Db or H-2Kk/Kd led to abrogation of CTL recognition (6,7). All these studies have been performed with CTL generated in bulk cultures and few long-term CTL clones.…”

mentioning

confidence: 99%

“…The properties of monoclonal antibodies used for the analysis of the transfected cells by the fluorescence activated cell sorter (FACS) are summarized in Table 1. FACS analyses were carried out as described (7).…”

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confidence: 99%

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Alloreactive cytolytic T-cell clones preferentially recognize conformational determinants on histocompatibility antigens: analysis with genetically engineered hybrid antigens.

Arnold¹,

Horstmann²,

Kuon³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Hybrid genes were constructed for the localization of allodeterminants on murine class I antigens recognized by antibodies and cytol ytic T lymphocytes. By using deletion subclones of the H12K and H-2Kk genes, homologous regions were exchanged between the two alleles. The altered genes were introduced and expressed in mouse fibroblast and fibrosarcoma cells. Cells expressing hybrid antigens were analyzed with 29 monoclonal anti-H-2Kd and anti-H-2Kk antibodies and with 150 short-term alloreactive cytolytic T-cell clones. When only the first or only the second amino-terminal domain was exchanged, most T cells and 60% of the antibodies lost their reactivity to the H-2K antigen. No T-cell clone was directed against the third extracellular domain, whereas three antibodies could bind to this domain. This implies that nearly all determinants essential for a cytolytic T-cell response or for antibody binding lie on the two external domains and are conformational structures generated by the interaction of these two domains.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Alloreactive cytolytic T-cell clones preferentially recognize conformational determinants on histocompatibility antigens: analysis with genetically engineered hybrid antigens.

Arnold¹,

Horstmann²,

Kuon³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Unrestricted recognition of class I neodeterminants generated by exon shuffling

1986

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We and others have previously reported that homologous exchange of alpha 1 or alpha 2 domains between different alleles led to loss of most determinants recognized by cytotoxic T lymphocytes (CTL) raised against the parental H-2 class I antigens. Here we demonstrate that exchange of alpha 1 or alpha 2 domains between the Kk and Kd allele results in the formation of neodeterminants against which allospecific CTL can be generated in responder mice of various related and unrelated H-2 haplotypes. In fact, only CTL against the neodeterminants on the hybrid molecules were found and none against the parental Kk and Kd determinants. In addition our data show that recognition of the neodeterminants is H-2 unrestricted. These findings suggest that in the hybrid molecules the basic structure of a major histocompatibility complex molecule has been preserved although most parental allodeterminants on the alpha 1 and alpha 2 domains have been modified.

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Role of the transmembrane and cytoplasmic domains of surface IgM in endocytosis and signal transduction

et al. 1992

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The cross-linking of membrane IgM (mIgM) triggers the activation and differentiation of B lymphocytes. One very rapid result of the cross-linking is the activation of phospholipase C, the subsequent mobilization of free calcium from internal stores and the activation of protein kinase C. This is followed by a redistribution of the receptor-ligand complexes to a small cap on the B cell surface, the first step in endocytosis and antigen processing. Cross-linking of major histocompatibility complex (MHC) class I neither stimulates the release of intracellular calcium nor does it induce capping and endocytosis of the cell surface receptors. In this study, we sought to determine the role of the two carboxyterminal domains of the mu heavy chain in signal transduction, capping and endocytosis of mIgM. We took advantage of the clear differences between MHC class I molecules and mIgM, replacing the transmembrane and cytoplasmic domains of mu by their MHC class I equivalents. Our results show that the hybrid heavy chain could still associate with light chains and assemble into a tetramer on the cell surface. However, cross-linking of the hybrid cell receptor produced neither release of calcium from internal stores, nor capping and endocytosis. These observations demonstrate that the two carboxy-terminal domains of mu are critical to both signal transduction and modulation of the mIgM-ligand complexes from the surface of B lymphocytes.

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Cytolytic T cells recognize the two amino-terminal domains of H-2 K antigens in tandem in influenza A infected cells

Cited by 77 publications

References 31 publications

Alloreactive cytolytic T-cell clones preferentially recognize conformational determinants on histocompatibility antigens: analysis with genetically engineered hybrid antigens.

Alloreactive cytolytic T-cell clones preferentially recognize conformational determinants on histocompatibility antigens: analysis with genetically engineered hybrid antigens.

Unrestricted recognition of class I neodeterminants generated by exon shuffling

Role of the transmembrane and cytoplasmic domains of surface IgM in endocytosis and signal transduction

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