Cytology of peritoneal aspirates and washings

Spriggs, A. I.

doi:10.1111/j.1471-0528.1987.tb02242.x

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…Of 24 cases with endometriosis or adenomyosis, 19 were entirely negative. In three cases a few cells resembling mesothelial cells reacted with HMFG-2 or E29, and in one of these a single mesothelial cell reacted weakly with Ca 2.…”

Section: Conditionsmentioning

confidence: 95%

Use of immunocytochemical staining to identify cells in peritoneal fluid and washings at laparoscopy and laparotomy.

Ghosh¹,

Spriggs²,

Charnock³

1986

Journal of Clinical Pathology

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Section: Conditionsmentioning

confidence: 95%

Use of immunocytochemical staining to identify cells in peritoneal fluid and washings at laparoscopy and laparotomy.

Ghosh¹,

Spriggs²,

Charnock³

1986

Journal of Clinical Pathology

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“…Smears of the deposits obtained by centrifugation of the specimens were stained with The finding of malignant cells in specimens obtained by peritoneal lavage in cases of ovarian carcinoma has been widely accepted as an important prognostic factor. 1,2 In 1985 the International Federation of Gynecology and Obstetrics (FIGO) adopted the use of the "C" subclassification in stage 1 and 2 ovarian carcinoma to reflect the presence of adenocarcinoma cells in ascitic fluid or peritoneal washings when evaluating disease progression. 3 In endometrial adenocarcinoma, however, the significance of malignant cells in peritoneal lavage specimens has been less clearly defined than in ovarian carcinoma.…”

Section: Methodsmentioning

confidence: 99%

Morphologic Analyses of Positive Peritoneal Cytology in Endometrial Carcinoma

et al. 1999

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“…However, both the false‐negative and false‐positive rates of peritoneal washing cytology are high 3, 6–15. These findings have led some researchers to question the value of peritoneal washing cytology 16…”

mentioning

confidence: 99%

Use of E-cadherin and CD44 aids in the differentiation between reactive mesothelial cells and carcinoma cells in pelvic washings

Chhieng

Yee

Cangiarella

et al. 2000

Cancer

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BACKGROUND The presence of malignant cells in peritoneal washings is an independent prognostic factor in the evaluation of gynecologic malignancies. The differentiation between reactive mesothelial cells and carcinoma cells can be a diagnostic challenge based on morphology alone. The expression of some cell adhesion molecules may be helpful in the differential diagnosis. METHODS To evaluate the specificity of 2 transmembrane cell adhesion proteins (E‐cadherin and CD44) in the differentiation of mesothelial cells from carcinoma cells in pelvic washings, formalin fixed, paraffin embedded cell blocks of pelvic washings from 19 cases of metastatic ovarian adenocarcinoma and 16 cases of benign peritoneal washings were immunostained with monoclonal antibodies to E‐cadherin and CD44. The staining patterns were evaluated blindly by three observers. Positive staining was defined as uniform membranous staining for each marker. RESULTS Fourteen benign peritoneal washings (87.5%) demonstrated immunoreactivity with anti‐CD44. On the contrary, only four adenocarcinomas (21.1%) demonstrated anti‐CD44 immunoreactivity. E‐cadherin expression was identified in only 2 benign peritoneal washings (12.5%) whereas 16 adenocarcinomas (84.2%) strongly expressed E‐cadherin. The differences in immunostaining for both CD44 and E‐cadherin between benign and malignant peritoneal washings were statistically significant. The combination of positive staining for E‐cadherin and negative staining for CD44 was 100% specific for metastatic adenocarcinoma, whereas a combination of negative staining for E‐cadherin and positive staining for CD44 was 100% specific for reactive mesothelial cells. CONCLUSIONS Both E‐cadherin and CD44 reliably distinguish reactive mesothelial cells from adenocarcinoma. The combination of E‐cadherin/CD44 is highly specific and is a useful diagnostic adjunct with which to distinguish benign reactive mesothelial cells from adenocarcinoma in pelvic washings. Cancer (Cancer Cytopathol) 2000;90:299–306. © 2000 American Cancer Society.

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Cytology of peritoneal aspirates and washings

Cited by 7 publications

References 16 publications

Use of immunocytochemical staining to identify cells in peritoneal fluid and washings at laparoscopy and laparotomy.

Use of immunocytochemical staining to identify cells in peritoneal fluid and washings at laparoscopy and laparotomy.

Morphologic Analyses of Positive Peritoneal Cytology in Endometrial Carcinoma

Use of E-cadherin and CD44 aids in the differentiation between reactive mesothelial cells and carcinoma cells in pelvic washings

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