Cytology of bacteria. II

Electron Microscopy of Ultrathin Sections of Bacteria Iii

“…Figure 14 indicates that the membrane septum (C.IS) forms by a centripetal growth from a ring of membrane material, in a manner similar to that in which the transverse cell wall forms in B. cereus, as was shown by Chapman and Hillier (1953). It should be noted that this method of formation of a membrane septum or "cell plate" was (lescribed by Knaysi (1941Knaysi ( , 1949Knaysi ( , 1951 and Robinow (1945). It is interesting to note that the "peripheral bodies" which apparently had a role in the formation of the transverse cell wall in B. cereus as reported by Chapman and Hillier (1953), do not appear in these cells which differ further from B. cereus in possessing a cytoplasmic membrane.…”

Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division

“…This report has presented the first high resolution cytological study of cytoplasmic membrane septation of bacteria. It should be pointed out, however, that Knaysi (1941Knaysi ( , 1949Knaysi ( , 1951, Robinow (1945), and Bisset (1948Bisset ( , 1950, on the basis of light microscope observations, gave remarkably similar descriptions of this phenomenon. Knaysi and Robinow described the inward annular growth of a ring from the cytoplasmic membrane to form a cell plate which was subsequently split by the centripetal growth of the cell wall.…”

A Procedure for the Simultaneous Demonstration of the Cell Walls and Chromatinic Bodies of Bacteria

“…Although reference is made herein to the free surfaces of the cells as walls it is well to keep in mind that these surfaces have been modified, by the hydrochloric acid treatment, from what, might be considered as their normal intact condition. According to Knaysi (1949), Dyar (1947) has made electrophoretic studies of the disintegrating action of hydr-ochlor-ic acid, at elevated temperatures, on the bacterial cell wall. The results showed a slow disintegrating action.…”