Cytology and pollen morphology of Bougainvillea glabra ‘Elizabeth Angus’, a cultivar with low pollen fertility

Zhang, Wei; Wen-ling, Guan; Li, Yefang; Peng, Lvchun; Zhang, Lu; Meng, Jing; Wang, Jihua; Song, Jié

doi:10.1016/j.scienta.2022.111105

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…CMS is defective in producing functional pollen, where pollen abortion happened at various developmental stages ( Zhang et al., 2022 ). Previous studies have suggested that 38 species exhibited pollen abortion during anther development, 15.5% of them happened at the early stages of meiosis (13.5% in dicotyledonous plants, 2% in monocotyledonous plants), 57.5% happened at the tetrad stage (29% in dicotyledonous plants, 28.5% in monocotyledonous plants), and 27% happened at the microspore developmental stage (7.5% in dicotyledonous plants, 19.5% in monocotyledonous plants; Wang et al., 2015 ; Liu et al., 2018b ).…”

Section: Introductionmentioning

confidence: 99%

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Ge,

Ding,

Daniel

et al. 2023

Front. Plant Sci.

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IntroductionCytoplasmic male sterility (CMS) is an important tool for hybrid heterosis utilization. However, the underlying mechanisms still need to be discovered. An adequate supply of nutrients is necessary for anther development; pollen abortion would occur if the metabolism of carbohydrates were hampered.MethodsIn order to better understand the relationship between carbohydrate metabolism disorder and pollen abortion in S-CMS wheat, the submicroscopic structure of wheat anthers was observed using light microscopy and transmission electron microscopy; chloroplast proteome changes were explored by comparative proteomic analysis; sugar measuring and enzyme assays were performed; and the expression patterns of carbohydrate metabolism-related genes were studied using quantitative real-time PCR (qRT-PCR) method.ResultsThese results indicated that the anther and microspore in S-CMS wheat underwent serious structural damage, including premature tapetum degeneration, nutritional shortage, pollen wall defects, and pollen grain malformations. Furthermore, the number of chloroplasts in the anthers of S-CMS lines decreased significantly, causing abnormal carbohydrate metabolism, and disintegration of osmiophilic granules and thylakoids. Meanwhile, some proteins participating in the Calvin cycle and carbohydrate metabolism were abnormally expressed in the chloroplasts of the S-CMS lines, which might lead to chloroplast dysfunction. Additionally, several key enzymes and genes related to carbohydrate metabolism were significantly inhibited in S-CMS.DiscussionBased on these results, we proposed a carbohydrate metabolism pathway for anther abortion in S-type cytoplasmic male sterility, which would encourage further exploration of the pollen abortion mechanisms for CMS wheat.

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Section: Introductionmentioning

confidence: 99%

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Ge,

Ding,

Daniel

et al. 2023

Front. Plant Sci.

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Cytological observations in abortion formation and morphological character of pollen in seedless chestnut rose (Rosa sterilis)

Du,

Zhou,

Zhong

et al. 2023

Flora

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Study of 15 Varieties of Herbaceous Peony Pollen Submicroscopic Morphology and Phylogenetic Relationships

et al. 2022

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Paeonia lactiflora Pall. is widely used in medicine, garden applications, and as a potted ornamental. Cultivated varieties of paeonifloras suitable for cut flowers are urgently needed. In this study, the pollen morphology of P. lactiflora was studied and the characters of different varieties were compared, so as to provide reference for selecting suitable parents for new hybrid varieties. We examined the pollen morphology of 15 herbaceous peony varieties using scanning electron microscopy and analyzed the external pollen morphology and genetic relationship of the varieties. The pollen grains of the studied varieties were spheroidal or subspheroidal, bilaterally symmetrical monads, circular in polar view, and circular or elliptical in equatorial view. The exine of the pollen grains was observed as being relatively smooth under the light microscope, with the area around the equatorial axis having more lumina under the scanning electron microscope. The pollen grain exine sculpture was either reticular or pit type. The pollen apertures were tricolporate, arranged longitudinally, and equally spaced. The pollen grains were of two sizes: medium and small. The differences between the varieties were mainly reflected in the exine sculpture of the pollen. The closer the genetic relationship between the 15 peony varieties, the more subtle the differences in the exine sculpture. In the same cluster group, the morphological characteristics of herbaceous peony pollen were correlated with the shapes of flower and scale buds and the texture of the petals. However, the study identified no direct correlation with the cultivar type and flower color.

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Cytology and pollen morphology of Bougainvillea glabra ‘Elizabeth Angus’, a cultivar with low pollen fertility

Cited by 3 publications

References 24 publications

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Cytological observations in abortion formation and morphological character of pollen in seedless chestnut rose (Rosa sterilis)

Study of 15 Varieties of Herbaceous Peony Pollen Submicroscopic Morphology and Phylogenetic Relationships

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